A Rome and Vatican Night Tour can be a fascinating and enchanting way to experience some of the most iconic and historic sites in Rome under the magical glow of the moonlight. Here's what you might expect from such a tour:

1. Colosseum: Your tour might begin at the Colosseum, an ancient amphitheater famous for its gladiatorial contests and other public spectacles. While you may not be able to enter the Colosseum at night, the exterior is often beautifully illuminated, providing a stunning sight against the night sky.

2. Roman Forum: The tour could also include a visit to the Roman Forum, the heart of ancient Rome. Illuminated ruins of temples, basilicas, and arches create a surreal ambiance, allowing you to imagine the bustling activity that once took place here.

3. Trevi Fountain: A stop at the Trevi Fountain is a must. Under the moonlight, the fountain's sculptures and cascading water take on a magical quality, and you might even have a chance to toss a coin over your shoulder into the fountain, a tradition believed to ensure a return to Rome.

4. Piazza Navona: This bustling square features stunning fountains, including the famous Four Rivers Fountain by Gian Lorenzo Bernini. The square is often lively in the evenings, with street performers and artists adding to the atmosphere.

5. Pantheon: The Pantheon, an ancient temple turned church, is a marvel of Roman engineering and architecture. Lit up at night, its dome and oculus create a mesmerizing play of light and shadow.

6. Vatican City: The tour might culminate with a visit to Vatican City, where you can marvel at the illuminated St. Peter's Basilica and St. Peter's Square. Although entry to the basilica itself may not be possible at night, the exterior is a sight to behold.

7. Sistine Chapel: Depending on the tour, you might also have the chance to visit the Sistine Chapel, where you can admire Michelangelo's masterpiece on the ceiling. The chapel's beauty is heightened under the soft lighting, allowing you to appreciate the intricate details of the artwork.

8. Nighttime Atmosphere: Throughout the tour, your guide might share historical and cultural insights about the sites you're visiting. You'll also have the opportunity to experience the unique atmosphere of Rome at night, with fewer crowds and a different perspective on the city's landmarks.

Remember that the specifics of the tour, such as the sites included and the availability of access to certain interiors, may vary depending on the tour operator and local regulations. It's a good idea to research and book your tour in advance to ensure a memorable and enjoyable experience.