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2024-02-09 12:12:27 -

Southern Blotting A Technique to Detect Specific DNA Sequences

Hybridization and DetectionAt this stage, a specific DNA probe labeled with a radiolabeled isotope such as 32P or non-radioactive markers is added to the membrane. The probe hybridizes or binds to any DNA fragments on the membrane containing the complementary base sequence. Unbound probe is washed off, and autoradiography or colorimetric/chemiluminescent detection methods are used to visualize...

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