A liquid is used to dissolve the biomolecules in the mobile phase, which is the second phase of Gel Permeation Chromatography. This allows for a strong detection response and wets the packing surface. A well-defined range of pore diameters may be found in the stationary phase, which is the first phase, which is made up of semi-permeable, porous polymer gel beads. It has qualities including a perfect and uniform porosity structure, chemical inertness and mechanical stability  a consistent porosity and particle size.

Syringe pumps and pumps that reciprocate featuring an extremely constant rate of flow are the two types of pumps used in this phase.Phase of the detectors - The detectors could be refractive index (RI) detectors, bulk property detectors, concentration-sensitive detectors.

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