A common analytical method for determining minute levels of chemicals in clinical applications, the environment, and food is called ELISA. Enzyme Linked Immunosorbent Assay is highly sensitive and selective. Based on ELISA, quick and high-throughput bioactive chemical detection using CD-shaped microfluidic devices constructed of glass and PDMS has been developed.

The device has 32 micro channels with an reaction micro chamber on each micro channel. Since fluid may move through each micro channel using centrifugal force, there are no valves or micro pumps are required. One device allows for the concurrent Enzyme Linked Immunosorbent Assay of 32 samples, making it simple to do high-throughput Enzyme Linked Immunosorbent Assay.