Enzyme Linked Immunosorbent Assay works on the principle of antibody-antigen interactions and uses an enzyme to produce a measurable color change corresponding to the amount of antigen present in the sample. The name Enzyme Linked Immunosorbent Assay comes from the key elements it uses - an antigen or protein is attached to wells of a plastic plate (immunosorbent), antibodies are added to bind to the antigen, and an enzyme linked to these antibodies produces a color change on reaction with a substrate (linked enzyme). The intensity of the color produced is directly proportional to the amount of antigen present.
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