Chemiluminescence Immunoassay: A Promising Instrument Unraveling the Potential of This Tool in Clinical Diagnostics
Chemiluminescence immunoassay (CLIA) is a sensitive laboratory technique used to detect the presence and quantity of antigens or antibodies in a biological sample. CLIA makes use of a chemical reaction known as chemiluminescence whereby light is emitted during the oxidation of a substance. This emitted light can then be measured by a luminometer and reported as a quantitative result.
How does CLIA Work?
In a CLIA, an antigen or antibody is linked to paramagnetic particles and incubated with the sample being tested. If the target antigen or antibody is present, it will bind to the Chemiluminescence Immunoassay analyte on the particles. After washing away unbound substances, a chemiluminescent-labeled antibody is added. This labeled antibody will bind to the analyte-particle complex if the target is present. After another round of washing, a triggering agent is added which causes the chemiluminescent molecule to emit a flash of light. The amount of light emitted is directly proportional to the amount of analyte present in the original sample. A luminometer detects and quantifies the amount of light and reports a result.
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