Unlocking the full potential of genomic engineering, especially with the versatile CRISPR/Cas9 system, is like discovering a new toolkit for everything from studying gene function to creating disease models and advancing drug research. Imagine the gRNA acting as a precise GPS for genes, and Cas9 as the tool to make exact edits—similar to fixing a detail in a blueprint. Unlike older systems like ZFN and TALEN, CRISPR/Cas9 offers a simpler and more efficient pathway to gene knockouts. Our scientists, well-versed in creating gene modifications, can guide you in using CRISPR to streamline research, including in challenging cases with tumor cells.
Genomic engineering in cell lines is a versatile tool for studying gene function, designing diseases models, biopharmaceutical research, drug discovery and many other applications. CRISPR (Clustered Regulatory Interspaced Short Palindromic Repeats)/Cas9 systems is a newly developed yet the most popular method for genome editing. It has been widely used in current biology, functional genome screening, cell-based human hereditary disease modeling, epigenomic studies and visualization of cellular processes.
CRISPR/Cas9 system consists of a “guide” RNA (gRNA) and a bacterial CRISPR-associated endonuclease (Cas9). The gRNA is a short synthetic RNA composed of a Cas9-binding “scaffold” sequence and ∼20 nucleotide “targeting” sequence that defines the target genomic site to be modified. Cas9 contains two nuclease domains to induce site-specific DNA cleavage. It’s a scalable genome-wide editing technology for its ease of generating gRNAs. The simplicity and high-efficiency of CRISPR/Cas9 system make it a preferable genomic knockout method to the traditional ZFN and TALEN system. Our scientists are experts at performing gene knockout with CRISPR/Cas9, from designing gRNA constructs to transfection and single clone generation of a wide range of cells, including difficult-to-transfect and tumor cell lines.
stable cell line generation
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