Aptamers have been used in affinity chromatography for the purification and isolation of target molecules from complex mixtures such as cell lysates or serum. Affinity chromatography based on aptamers offers high specificity, high recovery, and low non-specific binding. Aptamers can be easily immobilized on different matrices such as agarose, magnetic beads, or silica, enabling efficient purification of the target molecule.

Aptamers have been used in protein engineering for the design and development of novel proteins with improved properties such as stability, activity, or specificity. Aptamers can be used to target specific regions of a protein and modulate its activity or binding specificity. Aptamers can also be used as molecular scaffolds for the construction of novel protein structures or as building blocks for the design of protein-based nanomaterials.

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