CRISPR-Cas9 has changed the field of genome engineering. But, its use as a gene modifying software is limited by off-target outcomes and the prospect of lasting DNA damage. To deal with these limitations, analysts are suffering from a revised version of Cas9, known as dCas9. Unlike Cas9, which can cut DNA, dCas9 is catalytically inactive and alternatively binds to certain DNA sequences. This...
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