The "chain termination method," or Sanger sequencing, was created in 1977 by Frederick Sanger, an English biochemist, and his associates. This technique is intended to ascertain the nucleotide base sequence of a fragment of DNA, typically less than 1,000 bp in length. The "gold standard" for verifying DNA sequences, including those sequenced via next-generation sequencing (NGS), is Sanger...
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