Kiko Garcia

Kiko Garcia added blog Health
2024-12-09 08:04:56 -

Understanding Operational Taxonomic Units (OTUs) in Microbial Ecology
Operational Taxonomic Units (OTUs) serve as fundamental units in the realm of numerical taxonomy, particularly in the study of microbial ecology. These units can represent various biological classifications, including individuals, species, genera, or even higher taxonomic levels. The term "operational taxonomic unit" was introduced to address the discrepancies between traditional taxonomic...

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Kiko Garcia added blog Health
2024-12-09 08:00:26 -

Overcoming Data Quality Challenges in Next-Generation Sequencing for Metagenomics
Next-generation sequencing (NGS) has revolutionized the field of genomics by enabling researchers to generate vast amounts of sequence data in a single experiment. This technology is particularly valuable in applications such as metagenomics, where it allows for the analysis of complex microbial communities, such as those found in the human gut. However, despite its advantages, NGS is...

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Kiko Garcia added blog Health
2024-12-09 07:59:32 -

Mapping the Genome and Powering Biological Research
The advent of next-generation sequencing (NGS) technologies and advanced sequencers has led to an unprecedented surge in biological data generated by the scientific community. This explosion of information presents both remarkable opportunities and significant challenges for researchers. In this era of big data, bioinformatics has emerged as a crucial field, making substantial...

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Kiko Garcia added blog Health
2024-12-09 07:58:31 -

A Comprehensive Comparison of Illumina and Nanopore Sequencing Technologies
In the rapidly evolving field of genomics, sequencing technologies play a crucial role in advancing our understanding of genetic material. Among the most prominent methods are Illumina sequencing and nanopore sequencing, each offering unique advantages and capabilities that cater to different research needs. What is Illumina Sequencing? Illumina sequencing, a hallmark of...

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Kiko Garcia added blog Health
2024-12-09 07:58:29 -

A Comprehensive Comparison of Illumina and Nanopore Sequencing Technologies
In the rapidly evolving field of genomics, sequencing technologies play a crucial role in advancing our understanding of genetic material. Among the most prominent methods are Illumina sequencing and nanopore sequencing, each offering unique advantages and capabilities that cater to different research needs. What is Illumina Sequencing? Illumina sequencing, a hallmark of...

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Kiko Garcia added blog Health
2024-12-09 07:57:38 -

Exploring the Landscape of MicroRNA Targeting through Degradome Sequencing
Degradome sequencing, also known as parallel analysis of RNA ends (PARE) sequencing, is a cutting-edge technique that leverages high-throughput sequencing and bioinformatics to investigate the degradation products of mRNA. This method is particularly valuable for identifying the target genes regulated by microRNAs (miRNAs), which are small, endogenous non-coding RNAs approximately 22...

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Kiko Garcia added blog Health
2024-12-09 07:56:30 -

The Impact and Innovation of Single-Cell RNA Sequencing
Introduction to Single-Cell RNA Sequencing Single-cell RNA sequencing (scRNA-seq) represents a groundbreaking advancement in genomic techniques, allowing researchers to detect and quantify messenger RNA (mRNA) molecules at the single-cell level. This capability is crucial for understanding cellular responses and the intricate dynamics of biological systems. Traditional RNA sequencing methods...

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Kiko Garcia added blog Other
2024-10-22 01:34:31 -

Unveiling the Microbial World by Understanding the Diversity and Impact of Microorganisms
Unveiling the Microbial World by Understanding the Diversity and Impact of Microorganisms Microorganisms can be found everywhere, in the coldest or hottest environment, in the air, in water, in soil, in plants, and even inside the human body. These organisms have a big contribution to the ecosystem and to the biosphere as a whole. They have existed even before the earth was habitable for...

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Kiko Garcia added blog Health
2024-10-22 01:31:00 -

Mastering Microbial Cultivation by Navigating Aerobic and Anaerobic Growth Challenges
Microorganisms have different growth requirements. When culturing them in the laboratory, it is essential to take note of these conditions because these are needed to be replicated in order to successfully grow microbial cultures in the laboratory. As mentioned in the previous article, one of the conditions needed to be taken into consideration is the oxygen requirement of the...

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Kiko Garcia added blog Health
2024-10-22 01:29:04 -

How RNA Sequencing is Revolutionizing Drug Discovery and Development
In modern drug discovery and development, the research and development (R&D) process is a difficult task. The biological system is complex, which is one of the purposes. The human genome contains 20,000–25,000 genes. Traditional test methods for measuring gene expression levels or identifying gene isoforms, such as polymerase chain reaction (PCR), are expensive and...

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Kiko Garcia added blog Health
2024-10-22 01:23:25 -

Exploring How RNC-RNA Sequencing Illuminates Translational Dynamics and Molecular Mechanisms
When a ribosome is affixed to the polypeptide (protein) it is making, the ribosome-nascent chain complex (RNC) is the compilation of molecules that make up the ribosome. One of several methods can be used to halt the synthesis of the nascent polypeptide. RNCs are made and purified in labs to study the dynamics, biochemistry, folding, and interactions that the ribosome and proteins...

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Kiko Garcia added blog Health
2024-10-22 01:22:43 -

How to choose ANOVA?
Analysis of variance (ANOVA) is a powerful statistical method that allows researchers to compare the means of two or more groups and determine if they are significantly different. It is a popular tool for testing hypotheses in a variety of fields in biology, including ecology, genetics, physiology, and biochemistry. In this article, we will explore how to choose the right type of ANOVA for...

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Kiko Garcia added blog Health
2024-10-22 01:21:26 -

Sequencing Depth, Coverage and Read Types for NGS
What is sequencing depth and coverage? Sequencing Depth Sequencing depth is the ratio of the total number of bases (bp) obtained by sequencing to the genome size, which is one of the indicators to evaluate the sequencing volume. There is a positive correlation between sequencing depth and genome coverage, and the error rate or false positive results from sequencing decreases as the...

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Kiko Garcia added blog Health
2024-10-22 01:20:38 -

How to Submit Sequence Data to GenBank
Submission of sequence data to NCBI archives Next-generation sequencing, PacBio SMRT sequencing, and Nanopore sequencing, can generate numerous sequence data in a single run. Raw reads or assembled sequence need to be submitted to public sequence repository (DDBJ/ENA/GenBank - INSDC), which is required by the overwhelming majority of journals as accession numbers of theses...

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Kiko Garcia added blog Health
2024-10-21 04:01:59 -

Exploring the Hidden World of Viruses: Advances in Virome Research and Sequencing
Viruses Viruses, these minuscule and seemingly simple structures, are garnering increasing attention from scientists worldwide. With their unique characteristics and profound impact on living organisms, viruses are a source of fascination and complexity that extend far beyond their diminutive size. They are composed of a nucleic acid core, either DNA or RNA, encased within a protein coat....

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Kiko Garcia added blog Other
2024-10-21 04:01:07 -

Sequencing Technologies Unveiling Plant-Microorganism Interactions
What is the microbiome? The microbiome refers to a collection of microorganisms possessing distinct physicochemical characteristics, occupying well-defined and reasonable habitats. This term encompasses not only the microorganisms themselves but also their active zones, which establish specific ecological niches. This dynamic and interactive microecosystem undergoes changes...

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