Unveiling the Microbial World by Understanding the Diversity and Impact of Microorganisms
Microorganisms can be found everywhere, in the coldest or hottest environment, in the air, in water, in soil, in plants, and even inside the human body. These organisms have a big contribution to the ecosystem and to the biosphere as a whole. They have existed even before the earth was habitable for...
Microorganisms have different growth requirements. When culturing them in the laboratory, it is essential to take note of these conditions because these are needed to be replicated in order to successfully grow microbial cultures in the laboratory. As mentioned in the previous article, one of the conditions needed to be taken into consideration is the oxygen requirement of the...
In modern drug discovery and development, the research and development (R&D) process is a difficult task. The biological system is complex, which is one of the purposes. The human genome contains 20,000–25,000 genes. Traditional test methods for measuring gene expression levels or identifying gene isoforms, such as polymerase chain reaction (PCR), are expensive and...
When a ribosome is affixed to the polypeptide (protein) it is making, the ribosome-nascent chain complex (RNC) is the compilation of molecules that make up the ribosome. One of several methods can be used to halt the synthesis of the nascent polypeptide. RNCs are made and purified in labs to study the dynamics, biochemistry, folding, and interactions that the ribosome and proteins...
Analysis of variance (ANOVA) is a powerful statistical method that allows researchers to compare the means of two or more groups and determine if they are significantly different. It is a popular tool for testing hypotheses in a variety of fields in biology, including ecology, genetics, physiology, and biochemistry. In this article, we will explore how to choose the right type of ANOVA for...
What is sequencing depth and coverage?
Sequencing Depth
Sequencing depth is the ratio of the total number of bases (bp) obtained by sequencing to the genome size, which is one of the indicators to evaluate the sequencing volume. There is a positive correlation between sequencing depth and genome coverage, and the error rate or false positive results from sequencing decreases as the...
Submission of sequence data to NCBI archives
Next-generation sequencing, PacBio SMRT sequencing, and Nanopore sequencing, can generate numerous sequence data in a single run. Raw reads or assembled sequence need to be submitted to public sequence repository (DDBJ/ENA/GenBank - INSDC), which is required by the overwhelming majority of journals as accession numbers of theses...
Viruses
Viruses, these minuscule and seemingly simple structures, are garnering increasing attention from scientists worldwide. With their unique characteristics and profound impact on living organisms, viruses are a source of fascination and complexity that extend far beyond their diminutive size.
They are composed of a nucleic acid core, either DNA or RNA, encased within a protein coat....
What is the microbiome?
The microbiome refers to a collection of microorganisms possessing distinct physicochemical characteristics, occupying well-defined and reasonable habitats. This term encompasses not only the microorganisms themselves but also their active zones, which establish specific ecological niches. This dynamic and interactive microecosystem undergoes changes...
Microorganisms are ubiquitous in all kinds of environments on earth – they live in soil, water, inside or on the surface of animals and plants, and can even survive in extreme conditions. Microbiomics is the systematic study of the cohort of microorganisms in a given environment. With the advent of various sequencing technologies, researchers can finally get the whole picture of...
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