OBJECTIVE The rise of carbapenem resistance among Acinetobacter baumannii represents a challenge for the therapeutic management of infections. The present study aimed to investigate the sequence types and carbapenem resistance in A. baumannii strains collected from various clinical specimens from the patients admitted to tertiary care hospitals in Pakistan. METHODS A total of 156 A. baumannii clinical strains were analyzed for antimicrobial susceptibility, followed by genetic screening for the carbapenem-resistant determinants. All the A. baumannii strains were typed using multilocus sequence typing by the Pasteur scheme. RESULTS One thirty-nine of the 156 isolates (89.1%) were carbapenem-resistant and out of these 136 carried the blaOXA-23-like genes. Interestingly the sequence type (ST) 589 was the most common sequence type that was classified as clonal complex 1 (CC1). The ST2 was the second most common sequence type that corresponds to the clonal complex 2/92 (Pasteur scheme/oxford scheme), however, it was distributed in all the hospitals. CONCLUSION The diverse clones of carbapenem-resistant A. baumannii including the already reported STs as well as new STs carrying OXA-23 are mainly distributed in Pakistan. This is the first study that described the molecular epidemiology of widely disseminated A. baumannii in Pakistan. The findings will help to improve the knowledge of predominant sequence types and will be valuable for the deeper understanding of resistance mechanisms among various MLST types. The time-delay-based reservoir computing setup has seen tremendous success in both experiment and simulation. It allows for the construction of large neuromorphic computing systems with only few components. However, until now the interplay of the different timescales has not been investigated thoroughly. In this manuscript, we investigate the effects of a mismatch between the time-delay and the clock cycle for a general model. Typically, these two time scales are considered to be equal. Here we show that the case of equal or resonant time-delay and clock cycle could be actively detrimental and leads to an increase of the approximation error of the reservoir. In particular, we can show that non-resonant ratios of these time scales have maximal memory capacities. We achieve this by translating the periodically driven delay-dynamical system into an equivalent network. Networks that originate from a system with resonant delay-times and clock cycles fail to utilize all of their degrees of freedom, which causes the degradation of their performance. BACKGROUND Parechovirus-A3 (PeV-A3) and the enteroviruses (EVs) are the most common viral pathogens responsible for sepsis and meningoencephalitis in neonates and young infants; however, differences in the clinical presentations of two infections are not well described. https://www.selleckchem.com/products/pfi-2.html OBJECTIVES To describe the clinical presentations of PeV-A3- and EVs-related diseases and develop a novel scoring system to differentiate two diseases. STUDY DESIGN This prospective study used real-time PCR and genetic sequencing to evaluate viral etiologies of febrile neonates and infants less then 4 months with suspected sepsis or meningoencephalitis in Niigata area, Japan, in 2014-2016. The clinical manifestations of PeV-A3- and EVs-infected patients were compared, and a novel scoring system was developed after identifying the most distinguishable clinical findings, followed by the external cohort validation. RESULTS In 210 patients evaluated, we identified 56 PeV-A3-infected (27%) and 43 EVs-infected (20%) patients. The following clinical manifestations were significant in PeV-A3-infected patients, as compared with EVs-infected patients; a higher body temperature (38.9°C vs. 38.5°C, P  less then  .01) and heart rate (181/min vs. 168/min, P = .01), cold extremities (72% vs. 34%, P  less then  .01) and skin mottling (65% vs. 23%, P  less then  .01), lower white blood cell count (5,200/μL vs. 8,900/μL, P  less then  .01) and incidence of cerebrospinal fluid (CSF) pleocytosis (2% vs. 63%, P  less then  .01). Using some of these significant findings, the scoring system successfully distinguished the diseases (accuracy 86% and 83% for the derivative and external validation cohorts, respectively). CONCLUSIONS We found significant clinical manifestations in PeV-A3-infected patients compared to EVs-infected patients. The scoring system may be helpful to distinguish two infections, especially at onset of outbreak. The ability to repeatedly find exact the same nano region-of-interest (nROI) is essential for atomic force microscopy (AFM) studies of heterogeneous environmental samples. The large variety of methods makes it difficult to find the most suitable one for a specific research question. We thus conducted a literature research for nROI relocation methods and organized the found references in order to give an overview over relocation methods including the advantages, limitations and documented applications. This survey of nROI relocation methods and their key information facilitates the selection of appropriate methods with respect to a specific research question. Based on this survey, we developed a new AFM relocation approach urgently needed for the study of nano and micro sized particles and cells in air and aqueous environment. This approach uses commercially available TEM grids fully embedded in a semitransparent resin as a glue body on top of which particles and cells are fixed. Relocation of nROI within one grid is based on easily recognizable sample features in micro and nanometer scale. The stable sticking of the studied mineral particles and bacterial cells allows repeated measurements of the same nROI with differently functionalized tips in air as well as in water. Our simple, fast, and cost-effective method allows relocation with an accuracy of 10-40 nm and enables the implementation of AFM/ESEM correlative microscopy. This study investigated the effects of resveratrol and miR-22-3p on muscle fiber type conversion in mouse C2C12 myotubes. Here we showed that resveratrol significantly increased the protein level of slow myosin heavy chain (MyHC) and the activities of succinic dehydrogenase and malate dehydrogenase, as well as markedly decreased the protein level of fast MyHC and the activity of lactate dehydrogenase. Immunofluorescence staining showed that resveratrol remarkably upregulated the number of slow MyHC-positive myotubes and downregulated the number of fast MyHC-positive myotubes, suggesting that resveratrol promoted muscle fiber type conversion from fast-twitch to slow-twitch in C2C12 myotubes. We also showed that miR-22-3p had an opposite function on muscle fiber type conversion and resveratrol was able to repress the expression of miR-22-3p. Furthermore, AMP-activated protein kinase (AMPK) inhibitor Compound C and miR-22-3p mimics could attenuate and eliminate muscle fiber type conversion from fast-twitch to slow-twitch cause by resveratrol, respectively.

This study entailed a review of 63 scientific articles on geospatial and spatial-statistical analysis of the geographical dimension of the 2019 coronavirus disease (COVID-19) pandemic. The diversity of themes identified in this paper can be grouped into the following categories of disease mapping spatiotemporal analysis, health and social geography, environmental variables, data mining, and web-based mapping. Understanding the spatiotemporal dynamics of COVID-19 is essential for its mitigation, as it helps to clarify the extent and impact of the pandemic and can aid decision making, planning and community action. Health geography highlights the interaction of public health officials, affected actors and first responders to improve estimations of disease propagation and likelihoods of new outbreaks. Attempts at interdisciplinary correlation examine health policy interventions for the siting of health/sanitary services and controls, mapping/tracking of human movement, formulation of appropriate scientific and political responses and projection of spatial diffusion and temporal trends. This review concludes that, to fight COVID-19, it is important to face the challenges from an interdisciplinary perspective, with proactive planning, international solidarity and a global perspective. This review provides useful information and insight that can support future bibliographic queries, and also serves as a resource for understanding the evolution of tools used in the management of this major global pandemic of the 21 Century. It is hoped that its findings will inspire new reflections on the COVID-19 pandemic by readers.Clonal integration, i.e., resource sharing within clones, enables clonal plants to maintain biomass production when ramets (asexual individuals) under stress are connected to those not under stress. Oil pollution can strongly reduce biomass production, and connected ramets within clones may experience different levels of oil pollution. Therefore, clonal integration may help plants maintain biomass production despite oil pollution. Because biomass production is often negatively correlated with greenhouse gas emissions, we hypothesized that oil pollution would increase greenhouse gas emissions and that clonal integration would reduce such an effect. We tested these hypotheses in a coastal wetland dominated by the rhizomatous grass Phragmites australis near a major site of oil production in the Yellow River Delta in China. We applied 0, 5, or 10 mm crude oil per year for two years in plots within stands of P. australis and tested effects of severing rhizomes connecting ramets inside and outside a plot (i.e. preventing clonal integration) on biomass production, soil chemistry and greenhouse gas emissions. When severed, ramets inside plots with no added oil produced about 220 g aboveground biomass m--2 over the second growing season, and plots absorbed about 500 g total CO2 equivalents m-2. Adding 10 mm oil per year reduced aboveground biomass by about 30%, and caused plots to emit about 800 g CO2 equivalents m-2. Leaving ramets connected to those outside plots eliminated the negative effects of oil pollution on biomass production, and caused plots given 10 mm oil per year to emit about 50% fewer total CO2 equivalents. We conclude that oil pollution can increase greenhouse gas emissions and clonal integration can reduce the effect of oil pollution on biomass production and greenhouse gas emissions. Our study provides the first experimental evidence that clonal integration in plants can reduce greenhouse gas emissions.Agrarian landscapes theoretically provide ecosystem services that meet the demands of a wide range of socioecological processes. Consequently, any landscape agroecology approach must tackle the dynamic interaction of land-use distribution and associated social metabolism at different spatiotemporal scales. An agroecological scarcity case study explores how driven agricultural energy flows interact with landscape complexity in arid landscapes of 46 counties in the Qazvin Province (Iran). An Energy-Landscape Integrated Analysis (ELIA) was performed to correlate the energy reinvestment (E) and energy redistribution (I) present within the social metabolism network, with landscape complexity (Le) measured in terms of spatial patterns and related ecological processes. As well, a cluster analysis was run to establish agrarian landscape typologies based on the ELIA indicators. The results of this study provide an explicit sketch of the four strategies that society in Qazvin Province has developed within the dry environments that sustain it. Our findings confirm the hypothesis that there is a positive relationship between optimizing non-dissipative internal energy loops and landscape complexity, which can explain agroecosystem sustainability. This research enables us to define spatially informed agroecological transitions from a territorially explicit socioecological perspective and will make a significant contribution to decisions on agricultural policies given different land-use strategies, especially under scenarios of ecological scarcity.Taizhou is one of the three largest electronic waste (e-waste) recycling locations in China. At present, to prevent the environmental problems stem from e-waste dismantling, the local government shut down all the industries in 2015. https://www.selleckchem.com/products/pfi-2.html However, little is known to the sustaining effects of e-waste exposure on human health after the e-waste recycling factories were closed. In this study, we collected blood samples in the residents living near e-waste dismantling factories (exposed group) and a chosen reference area (reference group) for comparison in Taizhou in December 2017.17 metals were quantitatively determined in all blood samples. Among them, the concentrations of altogether 9 metals, including chromium (Cr), arsenic (As), cobalt (Co), nickel (Ni), silver (Ag), stannum (Sn), mercury (Hg), lanthanum (La) and cerium (Ce) were significant higher in the individuals in exposed group than in reference group. Hypothalamus-pituitary-adrenal axis (HPA axis) is critical endocrine system in regulating many physical functions.

Theoretical and empirical studies were examined to define distress, describe the nature and impact of the stress response, and determine how distress interacts with GRD, obesity, and advancing age. The literature was used to create a conceptual framework and model consisting of the interactions among the variables. Conclusion The Stress and Coping Framework for Type 2 Diabetes Mellitus shows how distress can contribute to the development of T2DM by interacting directly with established risk factors and in promoting insulin resistance.Aim To describe and analyse the PUs problem based on data from hospital electronic health records. Methods A retrospective cohort descriptive study was performed based on electronic health records (medical and nursing) from adult patients (≥18 years) admitted to medical, surgical and orthopaedics wards during 2016 after implementation of National Strategy of Patient Safety 2015-2020. Ethical approval was obtained. Results A sample of 3904 patients was obtained, 66% (n = 2575) were older than 65 years, 24.6% (n = 962) at admission and 21.2% (n = 829) at discharge stay at high risk of develop PUs and 88.6% (n = 3458) has no visual skin assessment. PUs identification, categorization and localization are inconsistent between nurses and doctors. Conclusion The creation of a unified minimum dataset for PUs monitoring to standardize data on the occurrence of PUs and assessed the effectiveness of preventive strategies in patient at risk of PUs development at national level is need. Current Portuguese guideline of PUs needs a review and an innovating upgrade with zero tolerance for PUs.Aim This study examined eating attitudes, perceptions of body image and quality of life of patients before and after bariatric surgery. Methods This prospective, descriptive study was conducted with fifty patients between April 23, 2018 and November 05, 2018. The data were collected by a survey form, the Three-Factor Eating Questionnaire, the Body Image Perception Questionnaire and SF-36 Quality of Life Questionnaire. The data collection instruments were applied before the surgery and at 1, 3 and 6 months following the surgery. Results The mean age of the patients was 36.34 ± 10.89 years, 78% were females, 44% were university graduates, 80% were married, 78% had a child, 70% were employed, 80% were morbidly obese before the surgery, and 36% were morbidly obese at the first month following the surgery. It was also determined that there was no morbid obesity among the patients, and 84% of the patients were obese at the third month following the surgery. At the sixth postoperative month, 24% of the patients were found to be obese, and 8% had normal weight. It was determined that the patients' eating attitudes and behaviours changed significantly after the surgery in comparison to their pre-surgery values (p = 0.001). The body image perceptions and quality of life of the patients during the postoperative period were found to be significantly higher in comparison to their preoperative values (p = 0.001). Conclusion This study showed that multidimensional evaluation of patients and implementation of nursing interventions contributed positively to individuals undergoing bariatric surgery based on healthy lifestyle behaviours.Background Communicating pain is a difficult endeavor due to the lack of observable pathology, the immeasurable nature of pain, and the presence of comorbid symptoms. While research has shown the value of cure-centered and care-centered communication, it is unclear how chronic pain patients would like to structure pain communication with their providers so that it produces pain disclosure and emotional support. Aim This study examines communication preferences of chronic pain patients including types of questions asked and information received to allow a holistic portrayal of the experience of living with chronic pain. Design The research used a quantitative survey that was disseminated via online chronic pain devoted support groups. Participants 192 respondents took the survey, with women respondents outnumbering men at a 41 ratio. Respondents came from 38 states and represented eight countries. Results For providers to better understand patients' chronic pain, results indicate they would like providers to inquire about how pain impacts their daily activities, relationships, work responsibilities, and goals and dreams using open-ended questions. These low-stake questions can facilitate emotional disclosure, increase feelings of support, and allow for co-morbid linkages. Conclusion These inquiries prioritize patients' own subjective knowledge, can deepen the communication exchange between provider and patient, and facilitate pain disclosure. The findings help to deliver patient-centered care, promote rapport, and foster trust between providers and their patients.Background Heart failure (HF) is associated with poor quality of life and increased morbidity and mortality. Aim This study aimed to investigate effect of application of Gordon's functional health pattern (FHP) model in nursing care of symptomatic HF patients on quality of life, morbidity and mortality in the post-discharge 30-day. Methods This is a prospective randomized controlled study conducted in a single center. Experimental group received nursing care planned in accordance with Gordon's FHP model. 60 control and 60 experimental HF patients were included in the study. In the control group nursing care was given according to the standard protocol of the hospital whereas in the experimental group nursing care was given in accordance with Gordon's FHP model. Patients in both groups were followed up after discharge at 30th day. Results Mean Minnesota Living with Heart Failure Questionnaire score improved significantly in the experimental group compared to the control group at 30th day (40.2 ± 23.5 vs 62.3 ± 22.9 respectively, p = 0.001). Seven patients (11.7%) in the experimental group and 17 patients (28.3%) in the control group were readmitted in the post discharge 30-day (p = 0.02). Kaplan-Meier survival curve analysis revealed significant difference in 30-day event free survival rates between groups (log-rank p = 0.31). https://www.selleckchem.com/products/hrs-4642.html Conclusion Application of Gordon's FHP model in the nursing care of HF patients was associated with significantly improved quality of life, and reduced hospital readmission rates at 30th day. This was the only independent predictor of 30-day event free survival.

The urgent need to address the high-cost issue of proton-exchange membrane fuel cell (PEMFC) technologies, particularly for transportation applications, drives the development of simultaneously highly active and durable platinum group metal-free (PGM-free) catalysts and electrodes. The past decade has witnessed remarkable progress in exploring PGM-free cathode catalysts for the oxygen reduction reaction (ORR) to overcome sluggish kinetics and catalyst instability in acids. Among others, scientists have identified the newly emerging atomically dispersed transition metal (M Fe, Co, or/and Mn) and nitrogen co-doped carbon (M-N-C) catalysts as the most promising alternative to PGM catalysts. Here, we provide a comprehensive review of significant breakthroughs, remaining challenges, and perspectives regarding the M-N-C catalysts in terms of catalyst activity, stability, and membrane electrode assembly (MEA) performance. A variety of novel synthetic strategies demonstrated effectiveness in improving intrinsic activg the comprehensive evaluation of M-N-C catalysts in MEAs. The most considerable challenge of current M-N-C catalysts is the unsatisfied stability and rapid performance degradation in MEAs. Therefore, we further discuss practical methods and strategies to mitigate catalyst and electrode degradation, which is fundamentally essential to make M-N-C catalysts viable in PEMFC technologies.Retinopathy of prematurity (ROP) is a blinding morbidity of preterm infants, which represents a significant clinical problem, accounting for up to 40% of all childhood blindness. ROP displays a range of severity, though even mild disease may result in life-long visual impairment. This is complicated by the fact that our current treatments have significant ocular and potentially systemic effects. https://www.selleckchem.com/products/neo2734.html Therefore, disease prevention is desperately needed to mitigate the life-long deleterious effects of ROP for preterm infants. Although ROP demonstrates a delayed onset of retinal disease following preterm birth, representing a potential window for prevention, we have been unable to sufficiently alter the natural disease course and meaningfully prevent ROP. Prevention therapeutics requires knowledge of early ROP molecular changes and risk, occurring prior to clinical retinal disease. While we still have an incomplete understanding of these disease mechanisms, emerging data integrating contributions of maternal/placental pathobiology with ROP are poised to inform novel approaches to prevention. Herein, we review the molecular basis for current prevention strategies and the clinical outcomes of these interventions. We also discuss how insights into early ROP pathophysiology may be gained by a better understanding of maternal and placental factors playing a role in preterm birth.Cranial radiotherapy has several side effects. One of the most important complications is radiation caries that endangers the treatment prognosis. In the literature, the use of crowns and bridges for irradiated patients has been suggested as a contraindication. In addition, due to the risk of osteoradionecrosis (ORN), there are doubts about tooth extraction and implant placement. Here, we present a treatment sequence and recalls for an irradiated young patient. For irradiated patients, it is recommended to replace teeth with implants when there is no possibility for supragingival prosthetic margin. Copyright© Dental Research Center, Tehran University of Medical Sciences.Objectives The dental profession has a special place of trust in the society, and dental clinicians must adhere to ethical standards in all dental procedures. Ethical conduct is one of the main expectations of individuals from this profession. The aim of this study was to design and develop dental ethical codes for national implementation in Iran. Materials and Methods This qualitative study was performed using directed content analysis method and purposive sampling. Data were collected until saturation through 15 semi-structured face-to-face individual interviews and two expert panels with academic staffs from dental faculties in Tehran, Iran. Results Data were classified into five principles and 90 codes. The principles included consideration of patients' interest as a priority, respect for human dignity and patient autonomy, confidentiality of patient information, the excellence of knowledge and skills, and building trust. Conclusion The ethical codes for Iranian dentists were drafted considering Islamic teachings and the prevailing culture. Some codes were exclusively developed for the cultural atmosphere of Iran especially on topics such as interaction with patients previously treated by other dentists. Some codes addressed the principles of consultation and continuing the therapeutic communication with such patients. Some items have not been considered in codes released by other associations, such as religious considerations in Islamic cover and alcohol consumption that were taken into consideration in this draft. These codes can serve as a guide for professional practice of dentists. It seems that these sets can help us reach the standardized code. Copyright© Dental Research Center, Tehran University of Medical Sciences.Objectives This study aimed to assess the effect of three commonly consumed beverages on surface roughness of polished and glazed zirconia-reinforced lithium silicate (ZLS) glass ceramics. Materials and Methods In this experimental study, 104 rectangular specimens were cut from Vita Suprinity blocks with 2 mm thickness. After ultrasonic cleaning and firing of the specimens, they were finished and polished in two groups. Specimens in the first group were polished using a 2-step polishing kit while the second group specimens were glazed and heated in a porcelain firing oven according to the manufacturer's instructions. Baseline surface roughness was measured using a profilometer. Specimens in each group were then randomly divided into 4 subgroups (n=13) for immersion in artificial saliva (control group), cola, orange juice and black tea. Surface roughness was measured again and data were analyzed using two-way ANOVA. Results The highest and the lowest mean Ra were found in orange juice and saliva subgroups, respectively in both glazed and polished groups.

The highest potency against BIR3 was observed among peptides containing C-terminal phosphonic phenylalanine analogs, which displayed nanomolar Ki values. Their antiproliferative potential as well as their proapoptotic action, manifested by an increase in caspase-3 activity, was examined using various cell lines.Background Non-prescription dispensing of antibiotics is common in Nigeria and this could contribute to the emergence of microbial resistance. Objectives To evaluate knowledge, perception and practices of community pharmacists towards dispensing antibiotics without prescription. Setting Community pharmacies in two cities in Northern Nigeria. Methods A prospective cross-sectional study was conducted among community pharmacists in two cities in Northern Nigeria, using a validated and pilot-tested questionnaire. The questionnaire was self-administered and data was collected between 06th April and 31st May 2019. The data was analyzed using descriptive and inferential analyses. Main outcome measure Knowledge, perception and practices towards dispensing antibiotics without prescription. Results A total of 98 out of 130 community pharmacists completed and returned the questionnaire (response rate 75.3%). About two-third (64.3%) of the community pharmacists were aware that dispensing antibiotics without prescription e main reason community pharmacists dispensed antibiotics without prescription. Continuous pharmacy education and training on handling of antibiotics may help to reduce inappropriate practices among community pharmacists.Background Signal generation through data mining algorithms is an innovative and emerging field in pharmacovigilance. Early detection of safety signals is important for public health safety. However, the possibility of generating pseudo signals should not be overlooked. Objective Our study aimed to identify potential signals of aromatase inhibitors associated Osteonecrosis of Jaw and assess the possibilities of the safety signal to be a pseudo signal/false positive in FDA Adverse Event Reporting System (FAERS). Setting Spontaneously reported data in FAERS database. Methods Data for this study were obtained from the public release of data in FAERS. OpenVigil, a pharmacovigilance analytical tool was used to access FAERS data. Reporting Odds Ratio (ROR) was used to assess the relation between the drug and adverse event. A value of ROR-1.96SE  > 1, (SE-standard error) was considered positive. Main outcome measure Signal strength. Results FAERS database had a total of 15,178 reports for Osteonecrosis of Jaw. Amongst which 617 reports were associated with aromatase inhibitors. Signal strength ROR (lower bound of the 95% CI) for letrozole, anastrozole and exemestane associated Osteonecrosis of Jaw without any background correction was 8.34, 6.64 and 15.14 respectively. Upon removing the reports of concomitantly administered drugs (bisphosphonates and denosumab), signal strength drastically decreased to 0.03, 0.36 and 0.47 for letrozole, anastrozole and exemestane respectively. The signal strength of bisphosphonates and denosumab associated Osteonecrosis of Jaw was not changed significantly upon removal of aromatase inhibitors. Conclusion Our study concluded that the signal generated for aromatase inhibitors associated Osteonecrosis of Jaw in FAERS database can be false positive. Careful background corrections with identification of those risk factors are imperative to exclude false positive results.A recent manuscript (Ferguson et al. in Impact of non-pharmaceutical interventions (NPIs) to reduce COVID-19 mortality and healthcare demand, Imperial College COVID-19 Response Team, London, 2020. https//www.imperial.ac.uk/media/imperial-college/medicine/sph/ide/gida-fellowships/Imperial-College-COVID19-NPI-modelling-16-03-2020.pdf) from Imperial College modelers examining ways to mitigate and control the spread of COVID-19 has attracted much attention. In this paper, we will discuss a coarse taxonomy of models and explore the context and significance of the Imperial College and other models in contributing to the analysis of COVID-19.A simple and efficient method for the synthesis of dihydro-8H-acenaphtho[1',2'4,5]pyrrolo[1,2-a]imidazole-diol derivatives via one-pot, four-component reaction of 1,1-bis(methylthio)-2-nitroethene, various amines, and acenaphthoquinone was developed. https://www.selleckchem.com/products/dn02.html All the reactions were carried out in ethanol at reflux without any catalyst. The main advantages of this method are good to high yields, experimental simplicity, mild reaction conditions, simple workup, and easy purification.INTRODUCTION Multiple studies have demonstrated that improved extent of resection is associated with longer overall survival for patients with both high and low grade glioma. Awake craniotomy was developed as a technique for maximizing resection whilst preserving neurological function. METHODS We performed a comprehensive review of the literature describing the history, indications, techniques and outcomes of awake craniotomy for patients with glioma. RESULTS The technique of awake craniotomy evolved to become an essential tool for resection of glioma. Many perceived contraindications can now be managed. We describe in detail our preferred technique, the testing paradigms utilized, and critically review the literature regarding functional and oncological outcome. CONCLUSIONS Awake craniotomy with mapping has become the gold standard for safely maximizing extent of resection for tumor in or near eloquent brain. Cortical and subcortical mapping methods have been refined and the technique is associated with an extremely low rate of complications.This is a personal narrative of my experience as a young man diagnosed with colorectal cancer. It is an exploration of the tension between multiple identities, including a clinical nurse specialist, academic and oncology patient. Young patients with colorectal cancer face particular challenges. They are often diagnosed with more advanced and poorly differentiated cancers. Research around young onset colorectal cancer is controversial, with some studies suggesting poorer survival. Although there is research in this area, there are few qualitative accounts that describe raw, honest and diverse narratives of colorectal cancer experiences, especially in young people. Writing personal narratives is a powerful experience, it has allowed me to tell my story to the world and given me time to reflect and make sense of my new reality. Although this journey has forced me to let go of my old identity, and embrace a new and challenging existence, the truth is that being diagnosed with cancer stinks. Writing this narrative is an uncomfortable process, punctuated with truths that are difficult to speak.

The current pandemic has rocked the lives of human beings every-where in ways never imagined, forcing us to question where our civilization is headed. In this article, we explore and discuss scien-tifi c evidence that helps explain recent events in the context of the COVID-19 pandemic.COVID-19 is caused by infection with a zoonotic-origin novel virus, SARS-CoV-2, that is genetically close to two coronavirus types iso-lated in bats. The transmission dynamics to humans from the original and intermediary hosts remain poorly understood, but it is highly likely that the SARS-CoV-2 virus infected humans after undergoing an inter-species transfer from bats to an intermediate species, and from there to human beings. Crossing the species barrier is largely fostered by industrial-scale agricultural practices that simplify original ecosystem connections by reducing biodiversity, facilitating the emergence of new infectious diseases. The scientifi c community has played an exemplary role in responding to this global emergency, working to fi nd timely, relevant solutions for governments and society as a whole. We need to take this opportunity to promote a global and open science that delves into the interrelation-ships of the biological, environmental, social and economic dimen-sions of this and other diseases while questioning current modes of production and their impact on the environment, and thus on human health worldwide. Keywords Coronavirus infections; communicable diseases; zoonoses; ecosystems; technology, industry, and agriculture; pandemics; global health; Mexico.Dr Barry is incoming Board Chair of the Consortium of Universities for Global Health (CUGH) and past President of the American Society of Tropical Medicine and Hygiene. She is a member of the National Academies of Sciences, Engi-neering, and Medicine, and serves on the National Academy of Medicine's Board of Global Health. She has received the Elizabeth Blackwell Medal for outstand-ing contributions to women in the fi eld of medicine, and is founder of WomenLift Health, a global initiative to transform global health outcomes by unleashing and elevating talented mid-career women to become global health leaders.Serving in her present role since the economic crisis of 2008, Alicia Bárcena is no newcomer to regional and global emergencies, economic or otherwise. She also has extensive experience in the UN system, including as chief of staff to the UN Secretary-General and later, during Ban Ki-moon's tenure in that position, as Under-Secretary General for Management. From 2016 to 2017, she co-chaired the International Resource Panel at the UN Environment Program. A biologist trained in her home country of Mexico, she later received a master's degree in public administra-tion from Harvard University, USA. For decades, Ms Bárcena has devoted her professional career to issues of sustainable development, fi nancing of public policies, and the environment. She has received Doctor Honoris Causa degrees from the University of Oslo, Norway (2014); the University of Havana, Cuba (2016); and the Universidad Autónoma de México (2019). Today, she is a board member of the Global Partnership for Sus-tainable Development Data and a member of the University of Oslo/The Lancet Independent Panel on Global Governance for Health.COVID-19 has upended the world's healthcare infrastructures and its economies, casting a glaring light on the failings and flaws already in place, all suffered unequally. This has forced leaders and the public at large to face the stark contrast between human society as it exists and the society that is possible, with both the world's majority and the planet leaping to the top of a new agenda...an agenda as urgently needed as a new vaccine.The appropriate timing of intervention and follow-up in asymptomatic patients with aortic stenosis remains controversial. https://www.selleckchem.com/products/baxdrostat.html Risk stratification is a key, especially with the use of a multimodality imaging approach, including exercise stress echocardiography. This review focuses on the use of exercise echocardiography in asymptomatic patients with moderate and severe aortic stenosis with preserved left ventricular ejection fraction. It describes the exercise echocardiography protocol, parameters to be evaluated, and its role in guiding the timing of intervention and follow-up in these patients.Objective Obstructive sleep apnea (OSA) is associated with elevated blood pressure (BP) and increases the risk of developing cardiovascular diseases. This study aimed to determine the clinical and polysomnographic features of OSA that are significantly associated with hypertension (HT). Methods This is a prospective study that enrolled patients diagnosed with OSA in Ankara University Faculty of Medicine from January 2015 to June 2016. The patients were categorized into normotensives (n=125) and hypertensives (n=141). BP was taken at the evening before and the morning after polysomnography (PSG). The polysomnographic findings of normotensive and hypertensive patients were compared, and independent risk factors that are associated with HT were analyzed. Results Hypertensive patients exhibited older age and higher Epworth sleepiness scale (ESS), apnea-hypopnea index (AHI), mean apnea duration, arousal index, and oxygen desaturation index (ODI) than normotensive patients. Nocturnal oxygen desaturation (NOD) was more frequent and the percentage of the duration of NOD to total sleep time (TST) was higher in hypertensive than normotensive patients. Multivariate analyses revealed that age (OR 1.095, 95% CI 1.053 to 1.139, p less then 0.001), ESS (OR 1.186, 95% CI 1.071 to 1.313, p=0.001), mean apnea duration (OR 1.072, 95% CI 1.032 to 1.113, p=0.002), ODI (OR 1.062, 95% CI 1.025 to 1.101, p=0.001), and NOD (OR 2.439, 95% CI 1.170 to 5.086, p=0.017) were independent risk factors for HT in OSA. Conclusion This study suggests that age, ESS, parameters of oxygenation, and apnea duration were associated with HT in patients with OSA. Hence, patients with OSA with these findings should be evaluated for HT. (Anatol J Cardiol 2020; 23 334-41).Objective We aimed to find crucial microRNAs (miRNAs) associated with the development of atrial fibrillation (AF), and then try to elucidate the possible molecular mechanisms of miRNAs in AF. Methods The miRNA microarray, GSE68475, which included 10 right atrial appendage samples from patients with persistent AF and 11 samples from patients with normal sinus rhythm, was used for the analysis. After data preprocessing, differentially expressed miRNAs were screened using limma. Target genes of miRNAs were predicted using miRWalk2.0. We then conducted functional enrichment analyses for miRNA and target genes. Protein-protein interaction (PPI) network and module analyses for target genes were performed. Finally, transcription factors (TFs)-target genes regulatory network was predicted and constructed. Results Seven genes, including CAMK2D, IGF2R, PPP2R2A, PAX6, POU3F2, YWHAE, and AP2A2, were targeted by TFs. Among these seven genes, CAMK2D (targeted by miR-31-5p), IGF2R (targeted by miR-204-5p), PAX6 (targeted by miR-223-3p), POU3F2 (targeted by miR-204-5p), YWHAE (targeted by miR-31-5p), and AP2A2 (targeted by miR-204-5p) belonged to the top 10 degree genes in the PPI network.

Deregulated genes were associated with cancer pathways and specifically extracellular matrix composition. Multiple collagen genes were more highly expressed in the BPA-exposed fibroblasts resulting in increased collagen deposition in the adult mammary gland. This transcriptional reprogramming of BPA-exposed fibroblasts generates a less permeable extracellular matrix and a stiffer mammary gland. These phenotypes were only observed in adult 12-week-old, but not 4-week-old, mice. Additionally, diethylstilbestrol, known to increase breast cancer risk in humans, also increases gland stiffness similar to BPA, while bisphenol S does not. CONCLUSIONS As breast stiffness, extracellular matrix density, and collagen deposition have been directly linked to breast cancer risk, these data mechanistically connect EDC exposures to molecular alterations associated with increased disease susceptibility. These alterations develop over time and thus contribute to cancer risk in adulthood.BACKGROUND Calcaneal Sanders type III or higher fractures traditionally have been treated with open reduction and internal fixation (ORIF); however, ORIF has associated complications. We investigated a combination of minimally invasive dual incision and internal fixation using mini plates for treating Sanders type III calcaneal fractures. METHODS Twenty patients with Sanders type III intra-articular calcaneal fractures with a posterior subtalar articular displacement > 2 mm were included. Surgical outcomes were assessed by visual analogue scale (VAS) pain score, American Orthopaedic Foot and Ankle Society (AOFAS) hindfoot score, and calcaneal geometry, including Böhler and Gissane angles. RESULTS The Böhler angle, Gissane angle, and height and length of the calcaneus were increased following treatment. Based on the AOFAS score, 80% of cases had excellent or good outcomes. The mean postoperative VAS pain score was 1.6. https://www.selleckchem.com/products/icarm1.html Complications such as malunion or a screw positioning deviation occurred in 6 patients, and one patient experienced delayed wound healing. There were no wound infections. CONCLUSIONS These results indicate that minimally invasive dual incision with mini plate internal fixation may be an effective alternative to ORIF for treating Sanders type III calcaneal fractures. Advantages include improvement of calcaneal geometry and a lower rate of wound infections.BACKGROUND Consumer mobility is an important aspect of a health insurance system based on managed competition. Both the general population and insured with a chronic illness should enjoy an equal opportunity to switch their insurer every year. We studied possible differences in the rates of switching between these two groups in the Netherlands. METHODS A structured questionnaire was sent to 1500 members of Nivel's Dutch Health Care Consumer Panel (response rate 47%) and to 1911 chronically ill members of the National Panel of the Chronically ill and Disabled (response rate 84%) in February 2016. Associations between switching and background characteristics were estimated using logistic regression analyses with interaction effects. RESULTS In general, we did not find significant differences in switching rates between the general population and chronically ill population. However, a combination of the population and background characteristics demonstrated that young insured with a chronic illness switched significantly less often than young insured from the general population (1% versus 17%). CONCLUSIONS Our results demonstrated that the group of young people with a chronic illness is less inclined to switch insurer. This observation suggests that this group might either face difficulties or barriers which prevents them from switching, or that they experience a high level of satisfaction with their current insurer. Further research should therefore focus on unravelling the mechanisms which explain the differences in switching rates.INTRODUCTION HIV-positive women who are still in the reproductive years need adequate sexual and reproductive health information to make informed reproductive health choices. However, many HIV-positive women who interface with the health system continue to miss out on this information. We sought to a) determine the proportion of HIV-positive women enrolled in HIV care who missed family planning (FP) counselling; and b) assess if any association existed between receipt of FP counselling and current use of modern contraception to inform programming. METHODS Data were drawn from a quantitative national cross-sectional survey of 5198 HIV-positive women receiving HIV care at 245 HIV clinics in Uganda; conducted between August and November 2016. Family planning counselling was defined as provision of FP information (i.e. available FP methods and choices) to an HIV-positive woman by a health provider during ANC, at the time of delivery or at the PNC visit. Analyses on receipt of FP counselling were done on 2760 HIV-m of FP counselling when they interfaced with the healthcare system. This presents a missed opportunity for prevention of unintended pregnancies, and suggests a need for the integration of FP counselling into HIV care at all critical time-points.BACKGROUND Endometriosis have a negative influence on women's sexual life. The aim of the current study was to test a conceptual model considering the interrelated role of anxiety, depression, sleep quality, physical activity, BMI, stage of endometriosis, the intensity of dyspareunia and pelvic pain on sexual function (SF) in infertile women with endometriosis. Also test the mediating role of sleep quality, anxiety, and depression. METHOD In the present cross-sectional study, 220 infertile women with a laparoscopically confirmed endometriosis were recruited. Data were collected using a socio-demographic checklist, Female Sexual Function Index (FSFI), Pittsburgh Sleep Quality Index (PSQI), Hospital Anxiety and Depression Scale (HADS), Visual Analog Scale (VAS). RESULTS We found that anxiety, depression, sleep quality, BMI, level of education, stage of endometriosis, and dyspareunia have a direct effect on women's SF. In our study, sleep quality, anxiety, pelvic pain, and depression were the four major mediators that the higher scores lead to a decrease in the SF of endometriosis patients.

Aims We aimed to investigate the functional alterations, diagnostic utilization, and prognostic implication of carotid arterial deformations in subjects with cardiovascular risk factors and heart failure (HF) with preserved ejection fraction (HFpEF). Methods and results Among 251 prospectively participants (mean age 66.0 ± 9.8 years, 65.7% female) in a single centre between December 2011 and September 2014, carotid artery deformations including circumferential strain (CCS)/strain rate and radial strain were analysed by two-dimensional speckle tracking. We further related these carotid artery deformation indices to HF biomarkers and cardiac structure and function by echocardiography and explored their prognostic values. Significant reductions of CCS, circumferential strain rate, and circumferential radial strain were observed across control (n = 52), high risk (n = 147), and HFpEF (n = 52) (trend P ≤ 0.001). Aging, hypertension, HFpEF, and higher pulse rate showed independent associations with reduced CCS by sovel mechanistic insights on functional arterial alterations reflecting coupled arterial-ventricular pathophysiology. Utilization of such measure may further provide additive prognostic value to advanced myocardial functional assessment.Background Male infertility represents a complex clinical condition requiring an accurate multilevel assessment, in which machine learning technology, combining large data series in non-linear and highly interactive ways, could be innovatively applied. Methods A longitudinal, observational, retrospective, big data study was carried out, applying for the first time the ML in the context of male infertility. A large database including all semen samples collected between 2010 and 2016 was generated, together with blood biochemical examinations, environmental temperature and air pollutants exposure. First, the database was analysed with principal component analysis and multivariable linear regression analyses. Second, classification analyses were performed, in which patients were a priori classified according to semen parameters. Third, machine learning algorithms were applied in a training phase (80% of the entire database) and in a tuning phase (20% of the data set). Finally, conventional statistical analyses were applied considering semen parameters and those other variables extracted during machine learning. Results The final database included 4239 patients, aggregating semen analyses, blood and environmental parameters. Classification analyses were able to recognize oligozoospermic, teratozoospermic, asthenozoospermic and patients with altered semen parameters (0.58 accuracy, 0.58 sensitivity and 0.57 specificity). Machine learning algorithms detected three haematological variables, that is lymphocytes number, erythrocyte distribution and mean globular volume, significantly related to semen parameters (0.69 accuracy, 0.78 sensitivity and 0.41 specificity). Conclusion This is the first machine learning application to male fertility, detecting potential mathematical algorithms able to describe patients' semen characteristics changes. In this setting, a possible hidden link between testicular and haematopoietic tissues was suggested, according to their similar proliferative properties.Biomedical magnesium alloy stents have become a hot bed of research focus in interventional therapy for nonvascular diseases. In this study, a numerical model for a balloon-expandable bile duct stent made of magnesium alloy with laser sculpture is developed to predict the effects of the degradation of the stent on the biomechanical behavior in the stent-bile duct coupling system. Based on a continuum damage model, the degradable model of the stent is built to understand its performance in an idealized bile duct as it is subject to corrosion over time. The degradation model developed in this study addresses the uniform corrosion and pitting corrosion. By means of the secondary development function of commercial numerical software ANSYS, the finite element analysis procedures were written to control the degradation process based on the technology of element "birth and death," and it is shown how the three-dimensional model and approach give the possibility of analyzing for the degradation mechanism of a magnesium alloy stent in the bile duct or other nonvascular cavities.Zein, a natural protein from corn, has important applications in food and pharmaceutical industries due the fact that it is biodegradable and biocompatible. However, due its relatively low mechanical properties and water solubility, many inorganic compounds (e.g., bioactive glasses [BGs]) have been used in combination with zein to obtain composite materials with improved mechanical properties. Such inorganic additions provide further biological functionality to zein. In this work, fiber mats of zein with incorporation of BG and copper doped BG particles are successfully obtained by electrospinning. At first the electrospinnability of the blends is assessed, then the morphological and chemical characterization of the mats is done. Degradation study in cell culture medium (Dubelcco's modified Eagle's medium) reveals a sufficient strength of the fibers, which in turn is necessary for in vitro cellular studies. Cell culture studies using MG-63 and C2C12 cells show promising results, demonstrating increased cell proliferation and growth for fiber mats containing both types of BGs. Also, evaluation with Staphylococcus aureus and Escherichia coli bacteria confirms the antibacterial activity of the scaffolds containing copper. The presence of Cu thus imparts antibacterial properties without influencing cell behavior. The developed electrospun fibers represent a novel scaffold system for tissue engineering applications.Background The flow cytometry analysis of GPI-linked proteins on red blood cells and leukocytes is crucial for paroxysmal nocturnal hemoglobinuria (PNH) diagnostics. However, the commonly used multicolor panels cannot be implemented in low-resourced hematology laboratories. In order to develop a simple prediagnostic test for PNH screening, we analyzed the diagnostic accuracy of the two-color (FLAER/CD15) detection of GPI-deficient neutrophils. Methods We reanalyzed multicolor data set of 1594 peripheral blood samples of patients screened for PNH applying only two markers (FLAER/CD15). The quantitative positivity/negativity was reported. https://www.selleckchem.com/products/hrs-4642.html Then, these results were compared in a blinded manner with previously obtained multicolor data from the same samples. Results Among the 1594 samples included in the study, 507 samples were PNH-positive by the multicolor assay. The two-color method revealed 510 PNH-positive samples. The detailed examination of this discrepancy revealed 12 false-positives and 9 false-negatives. Therefore, FLAER/CD15 screening method displayed 98.

The tandem duplication event just occurs in four gene pairs (Fv-RALF9 and Fv-RALF10, Md-RALF7 and Md-RALF8, Pm-RALF2 and Pm-RALF8, and Pp-RALF11 and Pp-RALF14) from four Rosaceae species. Our research provides a wide overview of RALF-like genes in seven Rosaceae species involved in identification, classification, structure, expression, and evolution analysis.The molecular heterogeneity of glioblastoma has been linked to differences in survival and treatment response, while the development of personalised treatments may be a novel way of combatting this disease. https://www.selleckchem.com/products/l-arginine-l-glutamate.html Here we show for the first time that low passage number cells derived from primary tumours are greater than an 86% match genetically to the tumour tissue. We used these cells to identify eight genes that could be used for the personalisation of glioblastoma treatment and discovered a number of personalised drug combinations that were significantly more effective at killing glioblastoma cells and reducing recurrence than the individual drugs as well as the control and non-personalised combinations. This pilot study demonstrates for the first time that whole exome sequencing has the potential be used to improve the treatment of glioblastoma patients by personalising treatment. This novel approach could potentially offer a new avenue for treatment for this terrible disease.Plant cell wall (CW) is a complex and intricate structure that performs several functions throughout the plant life cycle. The CW of plants is critical to the maintenance of cells' structural integrity by resisting internal hydrostatic pressures, providing flexibility to support cell division and expansion during tissue differentiation, and acting as an environmental barrier that protects the cells in response to abiotic stress. Plant CW, comprised primarily of polysaccharides, represents the largest sink for photosynthetically fixed carbon, both in plants and in the biosphere. The CW structure is highly varied, not only between plant species but also among different organs, tissues, and cell types in the same organism. During the developmental processes, the main CW components, i.e., cellulose, pectins, hemicelluloses, and different types of CW-glycoproteins, interact constantly with each other and with the environment to maintain cell homeostasis. Differentiation processes are altered by positional effect a. This information could be used for the rational design of genetic engineering traits that aim to increase the stress tolerance in key crops. Future growing conditions expose plants to variable and extreme climate change factors, which negatively impact global agriculture, and therefore further research in this area is critical.Potentiometric sensors have a great influence on the determination of most various compounds in their matrices. Therefore, efficient and new sensors were introduced to measure sodium Deoxycholate (NaDC) as a bile acid salt. These sensors are based on NaDC imprinted polymer (MIP) as sensory element. The MIP beads were synthesized using thermal polymerization pathway, in which acrylamide (AAm), ethylene glycol dimethacrylate (EGDMA), NaDC, and benzoyl peroxide (BPO) were used as the functional monomer, cross-linker, template, and initiator, respectively. The proposed sensors were fabricated using a coated screen-printed platform and the sensing membrane was modified by single-walled carbon nanotubes (SWCNTs) as an ion-to-electron transducer. The sensors exhibited high sensitivity that reached 4.7 × 10-5 M of near-Nernestian slope (-60.1 ± 0.9 mV/decade, r2 = 0.999 (n= 5)). In addition, the sensors revealed high selectivity, long lifetime, high potential stability, and conductivity that ensure reproducible and accurate results over a long time. MIP characterization was performed using Fourier Transform-Infrared (FT-IR) and a scanning electron microscope (SEM). Regarding the interaction of NaDC with serum albumin (SA), albumin is determined in human serum samples as human serum albumin (HSA), which was collected from different volunteers of different ages and gender.The increasing concern about noise pollution has accelerated the development of acoustic absorption and damping devices. However, conventional subtractive manufacturing can only fabricate absorption devices with simple geometric shapes that are unable to achieve high absorption coefficients in wide frequency ranges. In this paper, novel multi-layer micro-perforated panels (MPPs) with tunable wideband absorption are designed and fabricated by 3D printing or additive manufacturing. Selective laser sintering (SLS), which is an advanced powder-based 3D printing technique, is newly introduced for MPP manufacturing with polyamide 12 as the feedstock. The acoustic performances of the MPPs are investigated by theoretical, numerical, and experimental methods. The results reveal that the absorption frequency bandwidths of the structures are wider than those of conventional single-layer MPPs, while the absorption coefficients remain comparable or even higher. The frequency ranges can be tuned by varying the air gap distances and the inter-layer distances. Furthermore, an optimization method is introduced for structural designs of MPPs with the most effective sound absorption performances in the target frequency ranges. This study reveals the potential of 3D printing to fabricate acoustic devices with effective tunable sound absorption behaviors and provides an optimization method for future structural design of the wideband sound absorption devices."How do proteins fold?" Researchers have been studying different aspects of this question for more than 50 years. The most conceptual aspect of the problem is how protein can find the global free energy minimum in a biologically reasonable time, without exhaustive enumeration of all possible conformations, the so-called "Levinthal's paradox." Less conceptual but still critical are aspects about factors defining folding times of particular proteins and about perspectives of machine learning for their prediction. We will discuss in this review the key ideas and discoveries leading to the current understanding of folding kinetics, including the solution of Levinthal's paradox, as well as the current state of the art in the prediction of protein folding times.

Regarding their influence in sperm physiology, we hypothesize that these metabolites may explain the specific reproductive characteristics of each ejaculate portion. Finally, the reported SP metabolites could serve as a first steppingstone in the study of quality, functionality, and fertility biomarkers.Mutations in the gene encoding emerin (EMD) cause Emery-Dreifuss muscular dystrophy (EDMD1), an inherited disorder characterized by progressive skeletal muscle wasting, irregular heart rhythms and contractures of major tendons. The skeletal muscle defects seen in EDMD are caused by failure of muscle stem cells to differentiate and regenerate the damaged muscle. However, the underlying mechanisms remain poorly understood. Most EDMD1 patients harbor nonsense mutations and have no detectable emerin protein. There are three EDMD-causing emerin mutants (S54F, Q133H, and D95-99) that localize correctly to the nuclear envelope and are expressed at wildtype levels. We hypothesized these emerin mutants would share in the disruption of key molecular pathways involved in myogenic differentiation. We generated myogenic progenitors expressing wildtype emerin and each EDMD1-causing emerin mutation (S54F, Q133H, D95-99) in an emerin-null (EMD-/y) background. S54F, Q133H, and D95-99 failed to rescue EMD-/y myogenic differentiation, while wildtype emerin efficiently rescued differentiation. RNA sequencing was done to identify pathways and networks important for emerin regulation of myogenic differentiation. This analysis significantly reduced the number of pathways implicated in EDMD1 muscle pathogenesis.The aim of this work was to study the presence of Listeria monocytogenes, as well as the potential activity of two bioprotective cultures (Lyocarni BOX-74 and Lyocarni BOX-57), versus a mix of three L. monocytogenes strains that were intentionally inoculated in cooked cubed ham, packaged in Modified Atmosphere Packaging and stored at different temperatures. The bioprotective cultures limit L. https://www.selleckchem.com/products/l-ornithine-l-aspartate.html monocytogenes growth in cubed cooked ham stored either at 4 °C for 60 days and at 4 °C for 20 days and at 8 °C for 40 days. The inhibition at 8 °C is particularly useful for industrial cooked meat products, considering there are often thermal abuse conditions (8 °C) in the supermarkets. Both the starters can eliminate L. monocytogenes risk and maintain the products safe, despite the thermal abuse conditions. In addition, both culture starters grew without producing perceptible sensory variations in the samples, as demonstrated by the panel of the untrained tasters. The bioprotective LAB produced neither off-odours and off-flavours, nor white/viscous patinas, slime, discoloration or browning. Therefore, according to the obtained data, and despite the fact that cooked cubed ham did not show pH ≤ 4.4 or aw ≤ 0.92, or pH ≤ 5.0 and aw ≤ 0.94, as cited in the EC Regulation 2073/2005. It can be scientifically stated that cubes of cooked ham with the addition of bioprotective starters cultures do not constitute a favourable substrate for L. monocytogenes growth. Consequently, these products can easily fall into category 1.3 (ready-to-eat foods that are not favourable to L. monocytogenes growth, other than those for infants and for special medical purposes), in which a maximum concentration of L. monocytogenes of 100 CFU g-1 is allowed.Coronavirus disease 2019 (COVID-19) is a contagious disease caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV2), emerging in Wuhan, China and developing into a pandemic with rapidly emerging cardiovascular manifestations [...].This research reports the processability and mechanical properties of silk resins prepared by hot-pressing followed by hot-rolling and then analyzes their thermal and structural properties. The results show that regenerated silk (RS) resins are better suited for hot-rolling than Eri and Bombyx mori silk resins (untreated silk). When hot-rolling at 160 °C with a 50% of reduction ratio, maximum bending strength and Young's modulus of RS resin reaches 192 MPa and 10.2 GPa, respectively, after pretreatment by immersion in 40 vol% ethanol, and 229 MPa and 12.5 GPa, respectively, after pretreatment by immersion in boiling water. Increased strength of the material is attributed to the increased content of aggregated strands and intramolecular linking of β sheets (attenuated total reflectance Fourier-transform infrared spectroscopy) and higher crystallinity (X-ray diffraction analysis). After hot-pressing and hot-rolling, RS resins have a stable decomposition temperature (297 °C).This study aimed to examine the association between health insurance, city of residence, and outpatient visits among older adults living alone in China. A sample of 3173 individuals was derived from "Survey on Older Adults Aged 70 and Above Living Alone in Urban China" in five different cities. Logistic regression models indicated that older adults living alone who had urban employee basic medical insurance, urban resident basic medical insurance, and public medical insurance were more likely to have outpatient visits than those without any health insurance. After controlling the number of chronic diseases, only those with public medical insurance were more likely to have outpatient visits than uninsured older adults. Additionally, older adults who resided in Shanghai and Guangzhou were more likely to have outpatient visits than those in Chengdu, whereas older adults who were in Dalian and Hohhot were less likely to have outpatient visits. To improve the equity of outpatient visits among older adults living alone in China, policy efforts should be made to reduce fragmentation of different health insurance plans, expand the health insurance coverage for older adults, provide programs that consider the needs of this special group of older adults, and reduce the inequality in health resources and health insurance policies across cities.The four serotypes of dengue virus are the most widespread causes of arboviral disease, currently placing half of the human population at risk of infection. Pre-existing immunity to one dengue virus serotype can predispose to severe disease following secondary infection with a different serotype. The phenomenon of immune enhancement has complicated vaccine development and likely explains the poor long-term safety profile of a recently licenced dengue vaccine. Therefore, alternative vaccine strategies should be considered. This review summarises studies dissecting the adaptive immune responses to dengue virus infection and (experimental) vaccination. In particular, we discuss the roles of (i) neutralising antibodies, (ii) antibodies to non-structural protein 1, and (iii) T cells in protection and pathogenesis. We also address how these findings could translate into next-generation vaccine approaches that mitigate the risk of enhanced dengue disease. Finally, we argue that the development of a safe and efficacious dengue vaccine is an attainable goal.