A warming climate throughout the 21st century makes ambient high temperature exposure a major threat to population health worldwide. Mitigating the health impact of high temperature requires a timely, comprehensive and reliable assessment of disease burden globally, regionally and temporally. Based on Global Burden of Disease (GBD) Study 2019, this study aimed to evaluate the disease burden attributable to high temperature from various epidemiology perspectives. A three-stage analysis was undertaken to investigate the number and age-standardized rates of death and disability-adjusted life years (DALY) attributable to high temperature from GBD Study 2019. First, we reported the high temperature-related disease burden for the whole world and for different groups by gender, age, region, country and disease. Second, we examined the temporal trend of the disease burden attributable to high temperature from 1990 to 2019. https://www.selleckchem.com/products/ms4078.html Finally, we explored if and how the high temperature-related disease burden was modified provided a comprehensive understanding of the global disease burden attributable to high temperature, underscoring the policy priority to protect human health worldwide in the context of global warming with particular attention to vulnerable countries or regions as well as susceptible population and diseases. This study for the first time provided a comprehensive understanding of the global disease burden attributable to high temperature, underscoring the policy priority to protect human health worldwide in the context of global warming with particular attention to vulnerable countries or regions as well as susceptible population and diseases.The recent emergence of mobile-tigecycline resistance tet(X) genes in human and animals in China seriously threats the clinical utility of tigecycline. Here we focused on the isolation and molecular characterization of plasmid-mediated tigecycline resistance tet(X4)-positive E. coli from different sources in Pakistan using MinION and Illumina sequencing. The tet(X4) gene was detected in four E. coli isolates from poultry, chicken meat, wild bird and the slaughterhouse wastewater in Pakistan. Co-existence of colistin resistance mcr-1 gene was also detected in three isolates. The four isolates belonged to different sequence types and the tet(X4) gene was located on plasmids ranging from 12,331 bp to 113,610 bp belonging to IncFII and IncQ replicon types with two genetic contexts ISCR2-tet(X4)-abh-ISCR2-lysR-floR-virD2 and ΔISCR2-abh-tet(X4)-ISCR2-virD2-floR, respectively. In all the four E. coli strains, tet(X4) was transferable by conjugation to E. coli J53 host strain. In addition, three of four strains transferred tet(X4) to a wild-type carbapenem resistant E. coli strain. To our knowledge, this is the first report of the emergence of plasmid-mediated tet(X4) gene from Pakistan. The convergence of tigecycline and colistin resistance in South Asia is a serious threat to human health.Winter cover crop cultivation and its biomass recycling as green manure (GM) were accepted as an ideal nutrient management practice in temperate organic farming fields. Since its biomass growth was boosted with air temperature rising from late Spring to early Summer, this stage overlapped with cash crops' seeding or transplanting. Thus, organic farmers were suffering from low crop productivity, due to delayed mineralization of incorporated biomass. To accelerate the mineralization of biomass nutrients and control weeds, plastic film mulching (PM) was broadly utilized in organic farming fields of temperate-monsoon climate region. However, the effect of PM on increasing nutrient use efficiency was not properly quantified in GM amended soil. To determine the effect of PM on crop productivity and nutrient use efficiency in GM amended soils, PM and no-mulching treatments were installed under different levels of GM biomass amended soils (0, 25, 50 and 100% of harvested aboveground biomass). The biomass productivity of barley and hairy vetch mixture as cover crop and biomass nitrogen productivity were dramatically increased from the mid May to the early June. PM significantly improved soil temperature and moisture regimes during maize cropping seasons, and then increased inorganic N (NH4+ and NO3-) contents in soils. These improved soil properties under PM highly increased maize productivity and nitrogen use efficiency (NUE). Comparing with no-mulching, as GM application level was increased, the effect of PM on increasing maize productivity became greater, but its effect on increasing NUE became smaller. In conclusion, PM could be very useful tool to improve productivity and NUE of cash crop maize in organic cropping fields, in which the harvesting time of GM biomass might be sustained to increase GM biomass productivity.The contradiction between social economy and water environment has become increasingly prominent, and the analysis of urban water metabolism system (UWMS) represents a problem-solving approach from the perspective of the entire flow process. However, a comprehensive UWMS model that considers both physical and virtual water flows is currently lacking. This paper presents an innovative application of an ecological network model of the UWMS-integrated metabolic process of physical and virtual water in Xining during the 2002-2018 period. By analysing and screening the metabolic characteristics, metabolic structure and metabolic relationships, the sustainability of the UWMS is evaluated in depth, and the main causes and critical compartments of the unhealthy metabolic process are identified. The findings show that the UWMS in Xining maintains a moderate level of robustness (the average R = 0.48) with limited metabolic efficiency. Since 2012, the water management policies in Xining have been significantly strengthened, contributing to a slight increase in robustness by improving the water use efficiency and metabolic structure. The integrated metabolic process is unhealthy because the metabolic structure is not reasonable, and the systematic metabolic relationship tends to be antagonistic due to the network mutualism index dropping to 1.0 during 2016-2018. We conclude that efficient irrigation management, more infrastructure projects for the sewer network, wastewater treatment and recycling could provide effective support to enhance the coordinate development of the social economy and water environment in Xining.