Our study uncovered a promoting role of exogenous CCL2 on ovarian cancer cell proliferation through the MAPK/ERK signaling pathway, which may facilitate the discovery of more potential roles of CCL2 in ovarian cancer. The online version contains supplementary material available at 10.1007/s13205-020-02571-0. The online version contains supplementary material available at 10.1007/s13205-020-02571-0.Lentinus squarrosulus (Hed Khon Khao) is a source of bioactive polysaccharides. Three L. squarrosulus crude polysaccharides (LSPs) were subjected to cold water (LSP-CP), hot water (LSP-HP), and aqueous alkaline (LSP-AP) extractions, and their functional compositions and radical scavenging activities were compared. https://www.selleckchem.com/products/am-095.html Synchrotron radiation FTIR (SR-FTIR) spectra and PCA plot analysis in the bio-regions (4000-400 cm-1) revealed that functional composition LSPs differ significantly (P  less then  0.05). All LSPs had lipids, protein, and polysaccharides such as β-glucan. The major monosaccharides in LSP-CP and LSP-HP are d-galactose, d-glucose, and d-mannose at different proportions, while LSP-AP contained mainly d-glucose. Also, fucose and xylose were present in all the LSPs. LSP-CP, LSP-HP and LSP-AP induced maximum 1,1-diphenyl-2- picrylhydrazyl (DPPH) radical scavenging activity of 78.93 ± 0.42% at 3 mg/mL, 79.16 ± 1.43% at 3 mg/mL and 65.26 ± 1.74% at 5 mg/mL, whiles on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), their maximum activities were 98.94 ± 0.16% at 3 mg/mL, 97.42 ± 0.76% at 3 mg/mL and 47.24 ± 0.045% at 5 mg/mL, respectively. The results showed that LSP-CP and LSP-HP are good ABTS scavengers, whiles LSP-AP is poor ABTS scavenger. In overall, LSPs consist of essential functional compositions and could be used as natural antioxidants. This exploitation of fungal fruiting body extracts increased the potential use of L. squarrosulus in food and medicinal industries.The bacterial cellulose production (BCP) process, using cellulose microfibrils (CM) of Acetobacter xylinum enmeshed on luffa sponge matrices (LSM) as LSM-CM starter, has been successfully developed where the LSM-CM production process can be recycled through consecutive cycles in limited dissolved oxygen (DO) under continuous aeration. In this study, incremental aeration rates impacted the consecutive cycles. Gluconic acid production, during the process, resulting in the reduction of BCP, was increasingly generated at high aeration from 0.28 to 0.34% at 3 L/min to 0.83-0.97% and 1.52-1.99% at 6 and 9 L/min after 7 d culture at 30 ± 2 °C. To compensate for the negative impact of aeration, 0.10 and 0.15% (w/v) carboxymethyl cellulose (CMC) was found to create a microenvironment for recycled LSM-CM at both high aeration (6 and 9 L/min, respectively). Under nine consecutive BCP cycles, acceptable BC yields (from 5.54 ± 0.5 to 5.89 ± 0.5 g/L) were associated with high biomass at 6 L/min aeration. These results confirm that LSM-CM, combined with CMC called as LSM-CM-CMC, created microenvironments low in DO under high aeration rates and that the recycled LSM-CM-CMC with aeration is an alternative, sustainable, economic process that could be applied for mass BCP.The biochemical properties and anti-tumorigenic activity of Cerrena unicolor (CU) and Pycnoporus sanguineus (PS) towards colon cancer cells and the effect of supplementation of the fungal culture medium with cow milk on these activities were examined. CU1-II and PS4-II exhibited anticancer properties through various mechanisms. The extracts at the 200 µg/mL concentration significantly decreased the viability of HT-29 and SW948 cells. They also exhibited pro-apoptotic properties towards the cancer cell lines (HT-29, LS 180, and SW948). Furthermore, culturing the studied fungi on milk-supplemented media may improve the pro-health properties of both milk and mushrooms. The extracts had a higher concentration of proteins, lower levels of free amino acids, and higher content of phenolic compounds than milk. They also exerted a free radical scavenging effect, which may be connected with the high activity of catalase and superoxide dismutase. The tested extracts exhibited anticancer activity C. unicolor grown on the medium without milk and P. sanguineus grown on the medium with milk. The CU1-II and PS4-II extracts exhibited the strongest anticancer properties; however, PS4-II exerted a milder effect on normal CCD 841 CoTr cells than CU1-II. CU3-II exerted the mildest effect among all extracts on both normal and cancer cells.One of the most prevalent causes of bridge failure around the world is "scour"-the gradual erosion of soil around a bridge foundation due to fast-flowing water. A reliable technique for monitoring scour would help bridge engineers take timely countermeasures to safeguard against failure. Although vibration-based techniques for monitoring structural damage have had limited success, primarily due to insufficient sensitivity, these have tended to focus on the detection of local damage. High natural frequency sensitivity has recently been reported for scour damage. Previous experiments to investigate this have been limited as a result of the cost of full-scale testing and the fact that scaled-down soil-structure models tested outside a centrifuge do not adequately simulate full-scale behaviour. This paper describes the development of what is believed to be the first-ever centrifuge-testing programme to establish the sensitivity of bridge natural frequency to scour. A 1/60 scale model of a two-span integral bridge with 15 m spans was tested at varying levels of scour. For the fundamental mode of vibration, these tests found up to a 40% variation in natural frequency for 30% loss of embedment. Models of three other types of foundation, which represent a shallow pad foundation, a deep pile bent and a deep monopile, were also tested in the centrifuge at different scour levels. The shallow foundation model showed lower frequency sensitivity to scour than the deep foundation models. Another important finding is that the frequency sensitivity to "global scour" is slightly higher than the sensitivity to "local scour", for all foundation types. The level of frequency sensitivity (3.1-44% per scour depth equivalent to 30% of embedment of scour) detected in this experiment demonstrates the potential for using natural frequency as an indicator of both local and global scour of bridges, particularly those with deep foundations.