The aim of this research was to design an electrochemical immunosensor for determination of tumour necrosis factor receptor-associated protein-1(TRAP1) antigen, a heat shock protein linked to tumour necrosis factor. The indium-tin oxide covered polyethylene terephthalate (ITO-PET) electrode surface was cleaned and was prepared for the introduction of hydroxyl groups on its surface by using NH4 OH/H2 O2 /H2 O. As a silanization agent for covalent attachment of anti-TRAP1 on the surface of the ITO working electrode, 3-glycidoxypropyltrimethoxysilane (3-GOPS) was used. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to characterize the immobilization steps. A variety of parameters, 3-GOPS and anti-TRAP1 concentrations, and anti-TRAP1 and TRAP1 incubation durations were optimized. After determining the optimum conditions, characterization studies such as repeatability, reproducibility, regeneration, square wave voltammetry, and single frequency impedance were performed. The electrochemical immunosensor has presented an extremely wide determination range for TRAP1 from 0.1 pg/mL to 100 pg/mL.A set of catalysts having gold nanoparticles deposited on γ -Al2O3 ( Au/ γ -Al2O3) with lowest effective amount of gold content were prepared by successive impregnation and hydrogen reduction method. The structural features of prepared catalysts were analysed by X-ray diffraction (XRD), N2 physisorption, scanning electron microscopy (SEM), and Fourier transform infrared (FTIR). The catalytic activity was evaluated for the reduction of an organic pollutant 4-nitrophenol (4NP) to 4-aminophenol (4AP) by spectrophotometric analysis. Supported catalyst presented excellent catalytic ability to convert 4NP to 4AP in the presence of sodium borohydride (SBH) due to synergistic effect of Au NPs and mesoporous γ -Al2O3 support. The reduction reaction was also performed at a range of temperatures to calculate kinetic parameters. The development of highly stable Au/γ -Al2O3 catalysts with lowest noble metal content and recyclability made the process cost effective and may promote their applications in various fields including removal of organic pollutants in industrial waste water and high-temperature gas-phase reactions.In this paper, a fast, cheap, simple, sensitive and selective spectrophotometric method based on high spin peroxo-Fe(III)-EDTA complex in the alkaline medium was developed for the determination of hydrogen peroxide (H2O2) in real water samples. The purple-coloured complex with a maximum absorbance at a wavelength of 525 nm was formed. Various parameters such as type of stabilizer reagent and its concentration, reaction time, Fe(III), EDTA and NH3 concentration were optimized. The method was confirmed with the Beer's law with a molar absorption coefficient of 267.36 L mol-1 cm-1 in the 8.3 ×10-6 -4.08 ×10-3 mol/L concentration range. Sandell's sensitivity of the proposed method was also calculated as 0.188 μg/cm2 . LOD and LOQ were determined as 2.5 ×10-6 and 8.3 ×10-6 mol/L, respectively. Intraday and interday relative standard deviation of the proposed method for 2.0 ×10-4 mol / L of H2O2 were found as 1.5% and 6.1%, respectively. The developed method is suitable for fast monitoring of H2O2 in different types of aqueous water samples without any sample preparation steps and acceptable recovery values between 90% and 118% were obtained. In the sample analysis, H2O2 removed solutions from the real water samples were used for blank correction in their analysis and this process provides more reliable and accurate results in real sample analysis.The management of end-of-life tires (ELT) waste gains importance in aspect of possible environmental and economic issues so the waste recycling becomes unavoidable. This study describes the fabrication and characterization of a new phase changing material (PCM)/ELT microcomposites that could be used in thermal energy storage. Paraffin together with the 4 fatty acids and ELT rubber powder are used as PCMs and as the supporting material, respectively. Paraffin/ELT composites are fabricated, as well, by the vacuum impregnation method in order to investigate the effect of the preparation method. The thermal, morphological, and chemical properties of the prepared PCM/ELT rubber microcomposites are determined with differential scanning calorimetry (DSC), scanning electron microscopy (SEM), and FTIR, respectively. Additionally, the effects of the PCM amount on the composite materials are investigated. As a result of DSC results, the melting temperature and latent heat of the paraffin/ELT rubber microcomposites are determined as 37.2 °C and 80.79 J/g for direct impregnation method and 36.8 °C and 80.69 J/g for vacuum impregnation method, respectively. Based on the findings of this study, it can be claimed that PCM/ELT rubber microcomposites can be used as energy-saving materials in thermal energy storage applications.β -arrestins are responsible for termination of G protein-coupled receptor (GPCR)-mediated signaling. Association of single nucleotide variants with onset of crucial diseases has made this protein family hot targets in the field of GPCR-mediated pharmacology. However, impact of these mutations on function of these variants has remained elusive. In this study, structural and dynamical properties of one of β -arrestin2 (arrestin 3) variants, A248T, which has been identified in some cancer tissue samples, were investigated via molecular dynamics simulations. The results showed that the variant underwent structural rearrangements which are seen in crystal structures of active arrestin. Specifically, the "short helix" unravels and the "gate loop" swings forward as seen in crystal structures of receptor-bound and GPCR phosphopeptide-bound arrestin. Moreover, the "finger loop" samples upward position in the variant. Importantly, these regions harbor crucial residues that are involved in receptor binding interfaces. Cumulatively, these local structural rearrangements help the variant adopt active-like domain angle without perturbing the "polar core". https://www.selleckchem.com/products/n6f11.html Considering that phosphorylation of the receptor is required for activation of arrestin, A248T might serve as a model system to understand phosphorylation-independent activation mechanism, thus enabling modulation of function of arrestin variants which are activated independent of receptor phosphorylation as seen in cancer.