Besides, the results of microbial diversity showed that Fusarium and Fusicolla of BOF was nearly five times less than that in CK and chemical fertilizer groups, where the Bacillus content reached to three times as much of the CK. Moreover, the enzymes activity and the content of beneficial microorganisms in the rhizosphere increased significantly. In this study, the bioorganic fertilizer developed by the isolated strains had significant effects on the control of strawberry Fusarium wilt disease. Our results demonstrate that BOF is a promising approach to control this disease in strawberry production. © King Abdulaziz City for Science and Technology 2020.In this work, we present the draft genome sequence of Actinocorallia populi A251T consisting of 8,253,402 bp with a G + C content of 71.5 mol%. The genome sequence includes 3 5S rRNA genes, 54 tRNA genes, 28 snRNA genes and 19 CRISPRs in 26 contigs. Using the Rapid Annotation using Subsystem Technology, a total of 7766 coding DNA sequences, which were assigned to 436 subsystems, were found in the genome. And 6743 protein-coding sequences with designated functions were assigned to 19 categories in the Cluster of Orthologous Groups database. The genome contains 20 gene clusters responsible for the synthesis of secondary metabolites, including two type I polyketide synthase (PKS) gene clusters, two type III PKS gene clusters, three nonribosomal peptide synthetase (NRPS) gene clusters and two hybrid PKS/NRPS gene clusters. These results indicate that strain A251 has the ability to produce several nonribosomal polypeptides and diverse polyketides, which was verified by liquid chromatography-mass spectrometry analysis of the extraction from fermentation broth. In addition, lactones, indole and many unknown natural products are present in the metabolites. Bioactivity testing revealed anticancer and antidiabetic activities of the crude extract towards the cancer cell line HepG2. In summary, the genomic sequences and bioactive fermentation products demonstrate the potential of strain A251 in biotechnology. © King Abdulaziz City for Science and Technology 2020.Currently, there is a need of non-computationally-intensive bioinformatics tools to cope with the increase of large datasets produced by Next Generation Sequencing technologies. We present a simple and robust bioinformatics pipeline to search for novel enzymes in metagenomic sequences. The strategy is based on pattern searching using as reference conserved motifs coded as regular expressions. As a case study, we applied this scheme to search for novel proteases S8A in a publicly available metagenome. Briefly, (1) the metagenome was assembled and translated into amino acids; (2) patterns were matched using regular expressions; (3) retrieved sequences were annotated; and (4) diversity analyses were conducted. Following this pipeline, we were able to identify nine sequences containing an S8 catalytic triad, starting from a metagenome containing 9,921,136 Illumina reads. Identity of these nine sequences was confirmed by BLASTp against databases at NCBI and MEROPS. Identities ranged from 62 to 89% to their respective nearest ortholog, which belonged to phyla Proteobacteria, Actinobacteria, Planctomycetes, Bacterioidetes, and Cyanobacteria, consistent with the most abundant phyla reported for this metagenome. All these results support the idea that they all are novel S8 sequences and strongly suggest that our methodology is robust and suitable to detect novel enzymes. © King Abdulaziz City for Science and Technology 2020.Trans people are exposed to multiple human right violations in clinical practice and research. From 1975 on, gender transition processes have been classified as a mental disorder in diagnostic classification manuals, a classification that was removed recently from ICD, International Classification of Diseases, and continues in DSM, Diagnostic and Statistical Manual of Mental Disorders. https://www.selleckchem.com/products/gossypol.html Trans people in different world regions are forced to accept psychiatric diagnoses and assessment in order to get access to trans health care, subject to reparative therapies and exposed to transphobic institutional and social discrimination and violence. In many countries, gender identity laws include medical requirements, such as psychiatric diagnosis, hormone treatment, genital surgery, or sterilization. In the scientific literature, a frequent pathologization of trans experiences can be identified, by means of pathologizing conceptualizations, terminologies, visual representations, and practices, as well as ethnocentric biaobserved in the diagnostic classifications, as well as in the health care and legal context. At the same time, trans people continue being exposed to pathologization and transphobic violence. The Human Rights in Patient Care (HRPC) framework offers a human right-based approach on health care practices. The paper aims at analyzing the shared human rights focus and potential alliances between the trans depathologization perspective and the HRPC framework. © The Author(s). 2020.Purpose The aim of the present study was to analyze the associations of youths' sedentary behavior (SB) with parents' and siblings' SB and physical activity (PA), as well as the associations of youths' coparticipation with parents, siblings, and friends in PA and SB with youths' SB. Methods The sample consisted of 1543 youths (12.02 ± 2.51 years; 788 boys) enrolled in the baseline cohort of the UP&DOWN study. SB was assessed by accelerometry and questionnaire. Participants reported the time spent by their parents and siblings watching television, playing videogames, surfing the Internet, sitting/resting, and doing PA. Further, participants reported coparticipation with parents, siblings, and friends in these activities. Linear mixed models, including school and city as random effects, were performed. Results Parents' television time was positively associated with youths' screen-based SB. Coparticipation with friends in playing videogames (in boys) and in surfing the Internet (in girls) showed a positive association with screen-based SB and a negative association with educational-based SB. Moreover, coparticipation with siblings and friends in PA was inversely associated with accelerometer-based SB in boys and girls. Conclusion Our results emphasize the important role of social modeling in the development of sedentary lifestyles in youths. Interventions aimed at reducing health risk behaviors in youths could be more effective if they are oriented from a social perspective that involves their families and networks of their closest friends. © 2019 Published by Elsevier B.V. on behalf of Shanghai University of Sport.