f-MPC emulsions revealed higher viscosities compared to c-MPC emulsions, providing them improved stability. Viscosities of f-MPC emulsions were not significantly affected by heating at 90 °C for 30 min, while other emulsions exhibited a substantial increase in their viscosities due to protein denaturation and aggregation. Thus, f-MPC emulsions can be utilized in the development of protein-enriched functional foods (e.g., spreads) that are stable against high heat treatments.Herein, a novel electrochemical biosensor was constructed for the highly efficient detection of silver ions. A porous platform constructed with functionalized gold nanoparticles (AuPP) was electropolymerized on the gold electrode surface. The obtained polymer, analogous to a metal-organic framework, was used as the sensing platform together with cytosine-Ag+-cytosine interaction for dual signal amplification. The scanning electron microscopy (SEM) image of the AuPP platform exhibited a porous structure and considerable binding sites for C-riched single stranded DNA, leading to predictable silver ion preconcentration. Based on this strategy, the biosensor showed that the peak current in differential pulse voltammetry rose linearly as the concentration of silver ion increased from 0.005 to 3 μM with a detection limit of 1.3 nM. In addition, in the presence of other metal ions, such as Pb2+, Mn2+, Ni2+, Co2+, Cu2+, Zn2+, Na+, Ca2+, and Cd2+, at the same concentration, the current signal remained almost unchanged, manifesting high selectivity for Ag+. This proposed sensor might exhibit a novel fabrication method for metal ion detection with the aid of multiple AuPP materials by designing ligands with different functional groups.Quercetin has been proved to have high medicinal value, and as a type of flavonol, has been found in many plants. A simple, fast and effective detection of quercetin was developed according to the fluorescence enhancement by modified carbon dots (PBA-CDs). PBA-CDs were prepared by connecting phenylboronic acids (PBA) on the surface of carbon dots rich in amino groups. Due to the selective interactions of PBA with the cis-diol groups of quercetin and the additional hydrogen bonds, the intermolecular rotation of quercetin was inhibited, and then the fluorescence significantly increased. The selectivity of this method was proved and the linear ranges were estimated to be 0-28.5 μM with a limit of detection at 28.8 nM. Using this method, the quercetin content in the Ginkgo biloba leaf was measured as 42.28 mg·g-1. In addition, the PBA-CDs hold excellent biocompatibility and have demonstrated their ability to detect quercetin in living cells.Vibrational spectroscopy is considered a workhorse in heterogeneous catalysis. This group of techniques has allowed an understanding of the structure of the solid catalysts, not only with ex situ methodologies but also with in situ and operando studies. In this perspective, an appraisal of diffuse reflectance infrared Fourier transform spectroscopy (DRIFT) is provided, in particular regarding the heat transfer limitations in the cell, and solutions, which can affect the measurement, with consequences in kinetic studies.As newly emerging proton-conducting materials, metal-organic frameworks (MOFs) have been attracting wide attention in the field of proton exchange membrane fuel cells. However, for most of the MOF materials, long-term stability is a huge obstacle for practical applications. So, the structural stability of MOFs is the critical prerequisite for the design and development of modified materials with excellent proton conductivity. In this review, stable UiO-66 derivatives were chosen as the research object, and modification methods including post-synthesis modification and hybridization were mainly summarized. Based on the reported typical functionalization strategies, we found that the modified UiO-66 derivatives and their composite membranes demonstrate ultra-high proton conductivity similar to that of commercial Nafion, indicating their great application potential in fuel cells. This Frontier article focuses on the recent development in the modification of UiO-66 type frameworks and their composite membranes and the tuning of proton conductivity with structural factors.Antibiotics are the most commonly used clinical drugs for anti-infection, but they can also destroy normal microorganisms and cause intestinal barrier dysfunction. To elucidate the effects and mechanism of a water-soluble polysaccharide from Fagopyrum esculentum Moench bee pollen (WFPP) on intestinal barrier integrity in antibiotic-treated mice, BALB/c mice were exposed to a broad-spectrum antibiotic (ceftriaxone) or not (control), and were administered low-, medium- and high-dose WFFP (100 mg kg-1, 200 mg kg-1 and 400 mg kg-1, respectively) daily by oral gavage for 3 weeks. https://www.selleckchem.com/products/cyclo-rgdyk.html Mice treated with ceftriaxone displayed symptoms of growth retardation, atrophy of immune organs including thymus and spleen, increased gut permeability, and intestinal barrier damage, which were restored after intervention with WFFP at different doses. Moreover, the beneficial effects of WFFP were closely associated with enhanced intestinal sIgA secretion and reduced inflammatory response. Furthermore 16S rDNA gene sequencing revealed that WFPP elevated microbial diversity and richness and changed the community structure, therefore, alleviating microbiota dysbiosis caused by ceftriaxone. Interestingly, WFPP could modulate the abundance of sIgA secretion-related bacteria (e.g. Proteobacteria) and inflammation-related bacteria (e.g. Enterococcus). Therefore, WFPP can relieve antibiotic-induced microbiota dysbiosis to improve intestinal barrier integrity by increasing intestinal sIgA secretion and inhibiting inflammation.Four new water soluble Co(ii), Cu(ii) and Zn(ii) ionic metal complexes (1-4) [Cu(diimine)(H2O)2(glycinate)]+[glycinate]-, [Co(diimine)(H2O)4]+[glycinate]- and [Zn(diimine) (H2O)4]+[glycinate]-, where diimine = 2,2'-bipyridine (1-3) and 1,10-phenanthroline (4) were synthesized and thoroughly characterized by spectroscopic and single X-ray crystallographic studies. Complex 1 possesses a triclinic crystal system with a penta-coordinated geometry whereas complexes 2-4 crystallized in an isostructural monoclinic system having distorted octahedral geometry. Density functional theory (DFT) studies for complexes 1-4 were performed to correlate their geometrical parameters and to calculate the energy of frontier molecular orbitals. The corroborative results of spectroscopic and voltammetric studies with ct-DNA and tRNA revealed that the complexes bind noncovalently via an electrostatic mode of binding with specificity for tRNA as compared to ct-DNA. Gel electrophoresis experiments revealed that all the complexes unwind the plasmid pBR322 DNA at low micromolar concentrations (2-9 μM) following an oxidative mechanism for Cu(ii) and Co(ii) complexes (1, 2 and 4) whereas the Zn(ii) complex (3) mediates DNA cleavage by the hydrolytic pathway.