PKU#SW8.A simple biorefinery aimed at producing both biomethane (CH4) and polyhydroxyalkanoates (PHAs), was proposed to valorize the organic fraction of municipal solid waste (OFMSW). Anaerobic digestion (AD) was tested at different organic loading rates (OLR-I-II-III) (i.e. 3, 4 and 6 g L-1 d-1, respectively), producing biomethane and volatile fatty acids (VFAs)-rich digestate, the VFAs were then used to produce PHAs. https://www.selleckchem.com/products/7acc2.html Specific biogas and CH4 production remained similar when adopting different OLRs (biogas of 522-600 NL kg-1 VS and CH4 of 64-67% v/v). VFAs concentrated with OLR increases and their patterns were modified. PHA production was in the range of 117-199 g kg-1 OFMSWTS with the lowest production being associated to different polymer composition. The net energy recovery of this simple biorefinery accounted for 64% of OFMSW energy content, and the PHAs produced represented over 30% of the total energy.In the fermentation and bioenergy industry, terrestrial biomass is usually fractionated and the collected components, such as starch, are processed separately. Such a separation has not been reported for seaweeds. In this work, the direct hydrothermal processing of the whole green seaweed Ulva sp. biomass is compared to processing of separated starch and cellulose, to find the preferable route for monosaccharide, hydrochar, and polyhydroxyalkanoates (PHA) production. Glucose was the major released monosaccharide. A significant share of the glucose yield comes from the starch fraction. The highest hydrochar yield with the lowest ash content was obtained from the separated cellulose fraction. The highest PHA yield was obtained using a whole Ulva sp. hydrolysate fermentation with Haloferaxmediterranei. Economic analysis shows the advantage of direct Ulva sp. biomass fermentation to PHA. The co-production of glucose and hydrochar does not add significant economic benefits to the process under plausible prices of the two outputs.One of the goals of metabolic engineering is to engineer strains that can optimally produce target metabolites. However, the current workflow for rational engineering of the metabolic pathway is sometimes time-consuming and labor-intensive. Here, we have established a cost-effective approach for screening for variants secreting metabolites. Different surface display systems were adopted and verified, which anchored pHluorin to the Candida glabrata cell surface to associate pyruvic acid detection with the read out of this reporter. A generalizable simulation approach based on computational fluid dynamics and regularity of generated droplet dimension was presented, which was found to be an efficient design tool to explore microfluidic characteristics or optimization. Finally, a microfluidic platform based on simulation coupled with surface display system was constructed. A mutant exhibiting a 73.6% increase in pyruvic acid production was identified. This ultrahigh-throughput screening pattern offers a practical guide for identifying microbial strains with many traits of interest.Hydrothermal pretreatment can efficiently remove the residual antibiotics in oxytetracycline fermentation residue (OFR), but its effect on antibiotic resistance genes (ARGs) during composting remains unclear. This study compared the shifts in bacterial community and evolutions in ARGs and integrons during different composting processes of OFRs with and without hydrothermal pretreatment. The results demonstrated that hydrothermal pretreatment increased the bacterial alpha diversity at the initial phase, and increased the relative abundances of Proteobacteria and Actinobacteria but decreased that of Bacteroidetes at the final phase by inactivating mycelia and removing residual oxytetracycline. Composting process inevitably elevated the abundance and relative abundance of ARGs. However, the increase in ARGs was significantly reduced by hydrothermal pretreatment, because the removal of oxytetracycline decreased their potential host bacteria and inhibited their horizontal gene transfer. The results demonstrated that hydrothermal pretreatment is an efficient strategy to reduce the enrichment of ARGs during the OFR composting.The effect of co-digestion of food waste (FW) and cow dung (CD) with different ratios, and digestate recirculation with different recirculation ratios (RR) on the substrate degradation and energy production in continuous two-stage anaerobic fermentation system was investigated. Results from experiments indicated that co-digestion and digestate recirculation could promote the hydrogen production rate (HPR) and the methane production rate (MPR). Maximum HPR and MPR of 3.3 and 3.1 L/L/d were achieved for two-stage fermentation with recirculation system at RR of 0.4. Meanwhile, both co-digestion and digestate recirculation technology could reduce the amount of alkali addition to maintain pH in the hydrogen-reactor. Compared to digestate recirculation, co-digestion of FW and CD promote much more energy production, 654.9 and 4854.8 kJ/kgVSr were obtained from the co-digestion of FW and CD with the ratio of 21 in the hydrogen reactor and the methane reactor.This study aims to develop a spore fermentation method instead of fruiting body extraction for the production of Tremella aurantialba polysaccharide (TAPS). The purified spore strain Tremella aurantialba NX-20 was isolated for TAPS fermentation. The fermented TAPS and the extracted TAPS had the same monosaccharide composition but higher molecular weight. The conditions for TAPS fermentation by NX-20 were optimized, which were 25 °C, pH 7, 10% initial inoculum and 5 days. To further reduce the processing steps and costs of the medium, tofu wastewater (TW) was used to replace defatted soybean meal (DSM). In a 7.5 L fermentation tank, 40 g/L glucose with 10-fold diluted TW was used as the medium. For TAPS fermentation, a maximum yield of 15.02 ± 0.40 g/L was achieved. In conclusion, this study provides a feasible strategy for the efficient preparation of TAPS through spore fermentation.Anaerobic fermentation of microalgae was always hindered by its rigid cell wall structure. This paper reports a novel technique, i.e., adding potassium ferrate (K2FeO4) into microalgae fermentation systems to enhance short-chain fatty acids (SCFAs) production. The results showed that the maximum SCFAs production and acetic acid proportion were 732.6 mg COD/g VS and 54.6% at a dosage of 112.8 mg Fe(VI)/g VS, which were 168% and 208% of those in the control, respectively. Mechanism studies revealed that K2FeO4 effectively destroyed surface morphology and cell structure, and thus facilitated microalgae solubilization, providing a large number of biodegradable substrates for subsequent SCFA production. Although K2FeO4 inhibited all the microbial activities relevant to hydrolysis, acidification and methanogenesis processes to some degree, its inhibition to methanogens was much severer than that to other microbes. Illumina MiSeq sequencing analyses revealed that K2FeO4 addition increased the relative abundance (from 9.