Many remote montane ecosystems are experiencing biogeochemical changes driven by warming climate and atmospheric pollution. Compared with circumpolar and temperate lakes, the responses of subtropical montane lakes to these external stressors have been less investigated. Here we present sedimentary multi-proxies records (i.e. chironomids, elements and stable isotope of carbon and nitrogen) in 210Pb-dated cores from two montane ponds (central China). Before the 1900s, low biomass and the dominance of opportunistic species (e.g. Chironomus anthracinus-type) in both ponds might be in response to cold and harsh condition. Thereafter, chironomid communities in both ponds experienced pronounced shifts. Nutrient-tolerant/warm-adapted species (e.g. Chironomus sp., Polypedilum nubeculosum-type and Endochironomus impar-type) proliferated and biomass increased synchronously after the 1900s, suggestive of favorable condition for chironomid growth. Redundancy analyses revealed that changes in chironomid communities in both ponds were significantly correlated with rising temperature and δ15N depletion. Prolonged growing season and nitrogen subsidy would increase primary productivity, and hence enhancing food availability for chironomids. Catchment-mediated indirect effects of warming and nitrogen deposition, such as hydrological changes and terrestrial organic matter inputs, would impose further influences on chironomid communities. Taken together, the combined effects of climate warming and nitrogen deposition have caused significant shifts in primary consumers of these montane ponds, and imposed cascading effects on structure and function of subtropical montane aquatic ecosystems. Given the toxicity of metals, including aluminum (Al), and the effects of water temperature on ectotherms, we investigated the individual or association effect of these variables (Al + acidic pH + temperature changes) on sperm quality of Astyanax altiparanae. Mature males were divided into nine experimental groups based on the combination of each of three water temperatures (20, 25, and 30 °C) with neutral and acidic pH values (7.0 and 5.5, respectively) with or without 0.5 mg L-1 Al. The fish were subjected to subacute, semi-static exposure and at 24 and 96 h were evaluated for seminal parameters (1) pH; (2) osmolality; (3) sperm concentration; (4) sperm morphology; (5) sperm kinetics; and (6) sperm ultrastructure. At 30 °C, Al caused a reduction in osmolality (24 and 96 h) and sperm concentration (24 h). When analysing sperm kinetics (30 s post-activation), Al caused a reduction in total motility at all temperatures (24 h), and when this exposure time was longer (96 h), both acidic pH and Al addition to the water caused sperm motility reduction. By analysing curvilinear velocity (VCL) 30 s after sperm activation (24 and 96 h), the acidic pH caused a reduction in sperm movement at 20 and 30 °C, but at 25 °C Al triggered this reduction. Finally, Al in the water caused ultrastructural changes in the sperm head, midpiece, and flagella regardless of water temperature. Also, it was found that the combination of Al at 30 °C caused a reduction in sperm head area while at 20 °C, Al triggered a reduction in the midpiece area. Therefore, acidity influenced some A. altiparanae sperm parameters but Al in the water accentuated these effects on seminal quality, especially seminal osmolality and sperm concentration, kinetics, and ultrastructure. This toxicity was also influenced by changes in water temperature. The industrial and agricultural activities based on phosphorous can increase the F content in the surrounding area, causing a widespread adverse effect on the organisms. However, the current information on the superposed health risk posed by the multi-exposure to the F contamination in an area jointly affected by agricultural and industrial activities (DA) is limited. Herein, the F distribution in multi-environmental media and the exposure risk to humans by ingestion, inhalation, and dermal contact pathways are studied in an DA. The content of soil water-soluble fluorine (WF) was higher in the DA than in the area individually affected by agricultural activities (SA). This indicated a superposed contribution of the industrial and agricultural activities to increase the F toxicity in the soil. The correlation of the soil pH and the organic matter content with the soil WF concentration in DA suggested an inter-relationship between the soil physicochemical properties and the toxicity of F in the soil by industrial and agricultural activities. Irrigation water was not a major anthropogenic source of the cropland soil F. The large variation in F concentration in the crops (101.8-195.6%) might have originated from the discrepancies in the soil F content and air F concentration. The air F pollution (0.6-1.6 μg dm-2 d-1) in the area particularly influenced by intensive industrial activities should be important. The exposure of residents to F was mainly from the ingestion of F-enriched crops. The higher exposure of adults to F than that of children could be attributed to more industrial and agricultural outdoor activities, larger exposure area of the skin, and more daily ingestion of F-enriched food by adults. https://www.selleckchem.com/products/o6-benzylguanine.html Overall, present insights into the distribution of and the multi-exposure to F may be beneficial for decreasing the adverse F effects on the residents in DAs worldwide. The widespread use of rare earth elements (REEs) in numerous sectors have resulted in their release into the environment. Existing knowledge about the effects of REEs were acquired mainly based on toxicity tests with aquatic organisms and a fixed exposure time, Here, the dynamic accumulation and toxicity of REEs (La, Ce, and Gd) in soil organism Enchytraeus crypticus were determined and modeled by a first-order one-compartment model and a time-toxicity logistic model, respectively. Generally, the accumulation and toxicity of REEs were both exposure level- and time-dependent. The overall uptake rate constants were 2.97, 2.48, and 2.38 L kg-1d-1 for La, Ce, and Gd, respectively. The corresponding elimination rate constants were 0.99, 0.78, and 0.56 d-1, respectively. The worms exhibited faster uptake and elimination ability for light REEs (La and Ce) than for heavy REEs (Gd). For all three REEs, the LC50 values based on exposure concentrations decreased with time and reached ultimate values after approximately 10 d exposure.