before and after attending a breastfeeding training programme. Neonatal intensive care units need to increase their efforts to support breastfeeding. An important factor for mothers when establishing breastfeeding is support from well-trained professionals with a positive attitude to breastfeeding. The PreBAI could be a useful tool for identifying attitudes among HCPs before and after attending a breastfeeding training programme.The presented study is devoted to the investigation of the micellization-induced liquid-liquid fluctuations in sodium decanoate (NaD) aqueous solutions, based on the vibrational spectroscopic study of NaD and the determination of critical micelle concentration (CMC) of this system. At the same time, we focused on monitoring the effect of the addition of decanol to this system and changing its basic parameters from the point of view of CMC. CMC is an important parameter from a practical point of view and a characteristic feature of each micelle-forming compound. Upon analyzing the spectroscopic data we focus our attention mainly on the intensity and band position variations of both the symmetrical and antisymmetrical vibrational modes of CH2 groups situated in the high-frequency part of the spectrum. The study used normal (non-enhanced) Raman spectroscopy with excitation wavelength 785 nm, surface-enhanced Raman spectroscopy (SERS) on large-scaled gold-coated SERS-active substrates and infrared spectral measurements. The results of spectroscopic measurements were supported by tensiometry and potentiometry.Raman spectroscopy is a non-destructive technique utilizing lasers to observe scattered light in order to determine things such as vibrational modes in the molecular system. A major problem inherent to this technique is that due to their short exposure time and the low power of the excitation laser, Raman signals are very weak. They tend to be much weaker than the noise and can even be drowned out. Conventional denoising methods are currently unable to extract Raman peaks with precision so it is necessary to specifically study Raman signal extraction methods that involve a low signal-to-noise ratio (SNR). In this study, a denoising method for Raman spectra with low SNR based on feature extraction was proposed. Based on the Hilbert Vibration Decomposition (HVD) method, the Raman spectra was decomposed into two components. The peaks were located in the first component and compensated by those in the second component. Then based on the position and height of the peaks, their full widths at half maximum (FWHM) arrotene molecule, protein amide I, protein phenylalanine, nucleic acid cytosine, cellulose, DNA phosphodiester, RNA phosphodiester, D-glucose, α-D glucose, chlorophyll, lignin and cellulose were all accurate as well. The results from the simulation data and actual experiments show that a method based on feature extraction can effectively extract Raman peaks even when they are submerged in background noise. It should be noted that the practicality of this method lies in the fact that it requires few parameters and is simple to operate and implement.Fluorescence quenching of carbon dots (CDs) occurs in their aggregated state ascribed to direct π-π interactions or excessive resonance energy transfer (RET). Thus, CDs have been severely restricted for applications requiring phosphors that emit in the solid state, such as the fabrication of white light-emitting diodes (WLEDs). In this report, novel CDs with bright solid-state fluorescence (SSF) were synthesized by simple microwave-assisted synthesis method, using 1,4,7,10-tetraazacyclododecane (cyclen) and citric acid as precursors. Under 365 nm UV light, these CDs emit bright yellow SSF, indicating they successfully overcome the aggregation-induced fluorescence quenching (ACQ) effect. When the excitation wavelength (λex) is fixed at 450 nm, the emission peak of the CDs is centered at 546 nm with the Commission Internationale de l'Eclairage chromaticity (CIE) coordinates of (0.43, 0.55), which means that they can be combined with a blue-emitting chip in order to fabricate WLEDs. More importantly, the absolute quantum yield (QY) of these CDs powder reached 48% at λex of 450 nm, which was much higher than many previously reported SSF-emitting CDs and indicating their high light conversion ability in solid-state. Thanks to the excellent optical property of these CDs powder, they were successfully used in the preparation of high-performance WLEDs. This study not only enriches SSF-emitting CD-based nanomaterials with good prospects for application, but also provides valuable reference for subsequent research on the synthesis of solid-state fluorescent CDs.Fluorescent brighteners, illegally used to whitening wheat flour, are detrimental to people health. The aim was to establish a rapid and direct method to identify and quantify fluorescent whitening agent OB-1 (FWA OB-1) in wheat flour by using multi-molecular infrared (MM-IR) spectroscopy combined with stereomicroscopy. https://www.selleckchem.com/products/dansylcadaverine-monodansyl-cadaverine.html Characteristic peak profile of FWA OB-1 used as a judgment basis was spatially revealed by stereomicroscopy with group-peak matching of MM-IR at 1614 cm-1, 1501 cm-1 and 893 cm-1 and were further unveiled by the second derivative infrared spectroscopy (SD-IR) and its two-dimensional correlation infrared (SD-2DCOS IR) spectroscopy for higher resolution, and were validated by high-performance liquid chromatography (HPLC). Moreover, a quantitative prediction model based on IR spectra was established by partial least squares 1 (PLS1) (R2, 98.361; SEE, 5.032; SEP, 5.581). The developed method was applicable for rapid and direct analysis of FWA OB-1 (low to 10 ppm) in flour with relative standard deviation (RSD) of 5%. The capabilities of MM-IR with spectral qualitative and quantitative analysis would be applicable to direct identification and quantitation of fluorescent whitening agents or other IR-active compounds in powder objects.In this paper, an ultrasensitive and rapid "turn-on" fluorescence sensor, integrating flow-injection (FI) with nitrogen-doped carbon dots/gold nanoparticles (N-CDs/AuNPs) double-probe is established for the determination of metformin hydrochloride (MET) in biological fluids. The sensing strategy involves the weak inner filter effect between AuNPs and N-CDs due to aggregation products of MET with AuNPs. Unfortunately, the degree of AuNPs aggregation is difficult to control through manual assays, resulting in intolerable measurement error that limits further applications. However, the proposed method overcomes the above problem, and significantly lowers the consumption of expensive reagents (AuNPs about 60 μL per test). Under optimal conditions, the fluorescence intensity at 400 nm excitation and 505 nm emission wavelengths display a linear correlation with MET concentration (5-100 μg L-1) and the limit of detection is 2.32 μg L-1 (3.3 S/k). The advantages of the presented method include high sensitivity, rapid speed (60 sample h-1), good accuracy and precision (RSD ≤ 2.