a safe and effective method for access closure with sheath diameters up to and including 16 french. OBJECTIVE The aim of this study is to report our mid-term results of percutaneous arteriovenous fistula (pAVF) creation using the Ellipsys device and present technical recommendations and our algorithm of pAVF maintenance. METHODS A single center comprehensive database of all consecutive pre-dialysis and end-stage renal disease patients that had a pAVF creation with the Ellipsys® device was reviewed retrospectively. Study endpoints included technical success, maturation, functional patency and required interventions. RESULTS Between May 2017 and July 2019, 234 patients (mean age 64, 148 males - 63%) had a pAVF created. Technical success was achieved in 232 individuals (99%) and average duration of the procedure was 15 minutes (7-35minutes). Average follow up was 252 days (range 83-696). One year, primary, primary assisted and secondary patency were 54%, 85% and 96%, respectively. Average pAVF flow was 923ml/min (range 425-1440). There were no significant adverse events related to the procedure. Only 3 patients (1%) required a later conversion of the pAVF anastomosis to a surgical fistula. Twenty-four (10%) patients required superficialization of deep outflow veins due to difficult cannulation. Average maturation time was 4 weeks (range 1-12 weeks). Fourteen patients (6%) had early ( less then 2 weeks after creation) cannulation of their pAVF. CONCLUSIONS The Ellipsys® pAVF device allows for the rapid and safe creation of a reliable autogenous access. Rates of technical success, patency and maturation were excellent. For patients unsuited for a distal radio-cephalic arteriovenous fistula, it should be considered the next preferred access option. INTRODUCTION Ocular radiation exposure from fluoroscopically-guided-interventions (FGIs) can cause cataracts. Standard lead eyewear may not significantly reduce eye radiation dose, as the majority of scattered radiation penetrates the operator's eye obliquely. Our aim was to evaluate the efficacy of standard leaded eyewear and a customized eyewear design in lowering eye radiation dose to vascular surgeons. METHODS The attenuating efficacy of three forms of leaded eyewear (standard eyewear, eyewear with built-in leaded side-shields, and our modified eyewear) were tested in both a simulated setting and clinical practice. The modified design consisted of safety eyewear with 0.75 mm of added lead shielding attached to the lateral and inferior borders of the eyewear frame to attenuate oblique radiation. We performed simulated experiments using an anthropomorphic head phantom (ATOM model-701CIRS, Norfolk, VA) positioned to represent a primary operator performing right femoral access. Optically stimulated, luminescear led to a 62% left ocular radiation dose reduction (p less then .0001). CONCLUSIONS Standard lead-equivalent glasses are ineffective at reducing ocular radiation dose during FGIs. Eyewear modification with lateral and inferior lead shielding molded to the operator's face significantly decreases eye radiation exposure to the eye closest to the x-ray source. BACKGROUND The optimal strategy for revascularization in chronic limb-threatening ischemia (CLTI) is not yet completely known and is still under debate. Endovascular treatment methods predominate despite limited evidence for their advantage. In this concurrent, prospective observational cohort study we investigated outcomes after open and endovascular revascularization in the femoropoliteal segment due to CLTI. METHOD Between March 2011 and January 2015, 190 patients presenting with CLTI and the principal target lesion in the superficial femoral and/or popliteal segment underwent endovascular intervention (n=117) or bypass surgery (n=73) and were followed prospectively. The choice of revascularization technique was based on international and local guidelines. All patients were followed for two years. The primary endpoint was amputation-free survival assessed with Kaplan-Meier estimates, while secondary endpoints included CLTI symptom alleviation rates and re-intervention rates. A Cox proportional hazard regregher amputation-free survival than endovascular intervention, a finding that could not only be explained by differences in case mix. More patients who had bypass surgery were free from CLTI symptoms at both one and two years after revascularization. Re-interventions to maintain patency were equally common after bypass and endovascular intervention. ETHNOPHARMACOLOGICAL RELEVANCE Pinellia pedatisecta Schott extract (PE) is generated from Pinellia pedatisecta Schott, a traditional Chinese medicinal plant. PE suppresses cervical tumor growth and exhibits effects on dendritic cells (DCs) that lead to modulation of antitumor CD4+ and CD8+ responses. AIMS To explore the underlying mechanisms by which PE modulates tumor-associated dendritic cell (TADC) activation and function. METHODS DCs and TADCs were generated from murine bone marrow and exposed to PE solutions at different doses, as well as to repeated doses separated at different time intervals. Quantitative PCR, Western blot analysis, flow cytometry, and gene silencing were used to analyze the modulatory effects of PE on the SOCS1/JAK2/STAT pathways. Furthermore, we separated human cervical tumor-infiltrated DCs (TIDCs) and conducted an ex-vivo stimulation model to observe the effect of PE. For phenotypic analysis of cultured DCs and ex vivo human specimens, we used flow cytometry to detect the molecularreatment via the blockade of SOCS1 signaling in DCs. V.ETHNOPHARMACOLOGICAL RELEVANCE Yixin Ningshen tablet is a CFDA-approved TCM formula for treating depression clinically. However, little is known about its active compounds and related potential target proteins, so far, no researches have been performed to investigate its mechanism of action for the treatment of depression. https://www.selleckchem.com/products/AZD5438.html AIM OF THE STUDY Here we develop an original bioinformatics pipeline composed of text mining tools, database querying and systems biology combinatorial analysis, which is applied to rapidly explore the mechanism of action of Yixin Ningshen tablet in treating depression. MATERIALS AND METHODS Text mining and database query were applied to identify active compounds in Yixin Ningshen tablet for the treatment of depression. Then SwissTargetPrediction was used to predict their potential target proteins. PubMed was retrieved to summarize known depression related systems biology results. Ingenuity Pathway Analysis (IPA) tools and STRING were applied to construct a compound-target protein-gene protein-differential protein-differential metabolite network with the integration of compound-target interaction and systems biology results, as well as enrich the target proteins related pathways.