Biohydrogen production and integration possibilities are vital towards hydrogen economy and sustainability of the environment. Acidogenic fermentation is acquiring great interest and it is one of the prime pathways to produce biohydrogen and short chain carboxylic acids. In addition to hydrogen recovery, simultaneously nearly 60 percent of the organics may get converted to ethanol, 1,3propanediol and organic acids. Besides, these organics (fermentative effluents) can be used indirectly as a raw material for the generation of value- added products such as biolipid, polyhydroxyalkanoates, excess hydrogen, methane and electrical energy recovery. In this regard, this review has been assessed as a valuable biorefinery for biofuel and value- added products recovery. The biorefinery can be used to minimize entire cost of the approach by obtaining extra profits.This study was aimed at remediation ofoily waste contaminated area by utilizing a newly obtained bacterium. For experimental setup three different approachessuch as bioaugmentation, natural attenuation and abiotic factors were employed. In bioaugmented experimental set up (treatment withP. aeruginosaNCIM 5514),76.14 ± 0.85% loss in oily waste with notable hydrocarbon utilizers was noted in 56 days. From the results, it was concluded that bioaugmentation with novel P. aeruginosasp. (oily waste degrader) could remediate oily waste pollution effectively. Results of this study demonstrate applicability of P. aeruginosa NCIM 5514 for environmental sustainability.Protocatechuic acid (PCA) has been widely utilized in conventional pharmaceutical, cosmetic and functional food industries. Currently, chemical synthesis and solvent extraction are the main methods for commercial production, indicating several disadvantages. In this study, we developed a method for the biosynthesis of PCA in Pseudomonas putida KT2440 in high yield. First, we developed constitutive promoters with different expression intensities for fine-tuned gene expression. Second, we improved the biosynthesis of "natural" PCA in P. putida KT2440 via multilevel metabolic engineering strategies overexpression of rate-limiting enzymes, removal of negative regulators, attenuation of pathway competition, and enhancement of precursor supply. Finally, by further bioprocess engineering efforts, the best-producing strain reached a titer of 12.5 g/L PCA from glucose at 72 h in a shake flask and 21.7 g/L in fed-batch fermentation without antibiotic pressure. This was the highest PCA titer from glucose using metabolically engineered microbial cell factories reported to date.Lignin is the renewable and abundant source of aromatics on earth, and the depolymerization of lignin provides significant potential for producing valuable monophenols. In this work, catalytic hydrogenolysis of organosolv poplar lignin (OPL) in ethanol/isopropanol solvent over monometallic and bimetallic nonprecious catalysts was investigated. Ni/C and a series of NiCu/C catalyst with different Cu loadings were prepared and applied for depolymerization of OPL. The highest yield of phenolic monomers was 63.4 wt% achieved over the Ni10Cu5/C catalyst at 270 °C without external H2. The introduction of Cu in catalysts further promoted the hydrogen donor process of ethanol/isopropanol solvent and facilitated the cleavage of lignin linkages, resulting in the decreased molecular weight of bio-oil. The possible lignin dimer type structures, such as diphenylethane (β-1) type, phenylcoumaran (β-5) type, and pinoresinol (β-β) type structures, were proposed and identified by MALDI-TOF MS, giving a better understanding of the NiCu/C catalyzed lignin depolymerization.Syngas from biomass or steel mills can be fermented into a dilute stream of ethanol and acetic acid, which requires energy intensive distillation for product recovery. This can be circumvented by selective secondary fermentation of the syngas fermentation effluent to caproic acid as easier recoverable platform chemical with Clostridium kluyveri. https://www.selleckchem.com/products/deferoxamine-mesylate.html Here, we explore the impact of biochar and activated carbon on this process. Changes during the fermentation with biochar or activated carbon were monitored, different doses were tested and the recyclability of biochar and activated carbon was assessed. Biochar decreased the lag phase and increased the caproic acid production rate (up to 0.50 g·L-1·h-1). Upon recycling for subsequent fermentation, biochar retained this property largely. Activated carbon addition, especially at high dose, could potentially increase the conversion and selectivity towards caproic acid to 14.15 g·L-1 (control 11.01 g·L-1) and 92% (control 84%), respectively.The co-production of industrially relevant biopolymers/biomolecules from microbes is of biotechnological importance. Herein, a unique bacterium, Iodobacter sp. PCH 194 from the kettle lake at Sach Pass in western Indian Himalaya was identified. It co-produces biopolymer polyhydroxyalkanoates (PHA) and biomolecule (violacein pigment). Statistical optimization yielded dual products in the medium augmented with glucose (4.0% w/v) and tryptone (0.5% w/v) as carbon and nitrogen sources, respectively. The purified PHA was polyhydroxybutyrate (PHB), and pigment constitutes of violacein (50-60%) and deoxyviolacein (40-50%). A bench-scale bioprocess in 22.0 L fermentor with 20% dissolved O2 supply produced PHB (11.0 ± 1.0 g/L, 58% of dry cell mass) and violacein pigment (1.5 ± 0.08 g/L). PHB obtained was used for the preparation of bioplastic film. Violacein pigment experimentally validated for anticancerous and antimicrobial activities. In summary, a commercially implied bioprocess developed for the co-production of PHB and violacein pigment using the Himalayan bacterium.This two-phase, two-stage study analyzed production of biohydrogen and volatile fatty acids by acidogenic fermentation of brewery spent grains. Phase-1 served to optimize the effect of pH (4-10) on acidogenic fermentation; whereas phase-2 validated the optimized conditions by scaling up the process to 2 L, 5 L, and 10 L. Alkaline conditions (pH 9) yielded excellent cumulative H2 production (834 mL) and volatile fatty acid recovery (8936 mg/L) in phase-1. Extended fermentation time (from 5 to 10 days) upgraded the accumulated short-chain fatty acids (C2-C4) to medium-chain fatty acids (C5-C6). Enrichment for acidogens in modified mixed culture improved fatty acid production; while their consumption by methanogens in unmodified culture led to methane formation. Increased CH4 but decreased H2 content enabled biohythane generation. Scaling up confirmed the role of pH and culture type in production of renewable fuels and platform molecules from brewery spent grains.