As a macrolide antibiotic, clarithromycin (CLA) has a high detection rate in surface water and sewage treatment plant effluents worldwide, posing a considerably high ecological risk to aquatic ecosystem. However, algal transcriptome and metabolome in response to CLA remains largely unknown. In this study, a model alga Raphidocelis subcapitata (R. subcapitata), was exposed to CLA at the concentrations of 0, 3, 10, and 15 μg L-1. Transcriptomic analysis was performed for all the treatment groups, whereas metabolomics was merely applied to 0, 3, and 10 μg L-1 groups because of the limited amount of algal biomass. After 7 d cultivation, the growth of R. subcapitata was significantly hindered at the concentrations above 10 μg L-1. A total of 115, 1833, 2911 genes were differentially expressed in 3, 10, and 15 μg L-1 groups, respectively; meanwhile, 134 and 84 differentially accumulated metabolites (DAMs) were found in the 3 and 10 μg L-1 groups. Specifically, expression levels of DEGs and DAMs related to xenobiotic metabolism, electron transport and energy synthesis were dysregulated, leading to the produced reactive oxygen species (ROS). To confront the CLA-induced injury, the biosynthesis of unsaturated fatty acids and carotenoids of R. subcapitata in 3 μg L-1 were up-regulated; although the photosynthesis was up-regulated in both 10 μg L-1 and 15 μg L-1 groups, the energy synthesis and the ability to resist ROS in these two groups were down-regulated. Overall, this study shed light on the mechanism underlying the inhibitory effects of macrolide antibiotics in algae.Mercury pollution has pervaded many artisanal gold mining communities in the world, especially in developing countries. This study aimed to evaluate the potential risk of mercury pollution in soils in Gbani, an artisanal gold mining community in Ghana. Two hundred and thirty-seven soil samples were collected from within 0-10 cm depth, from active mining sites near residences, two transects in the community, waste soil from mining processing and the surroundings of the community. The measured mean mercury soil concentrations were 71 mg Hg/kg in active mining sites, and more moderate (2.7 mg Hg/kg) along transects through the community. Enrichment Factors classified the soils of the study area as being moderately to extremely severely contaminated with mercury. The spatial distribution shows the contamination of mercury is highest at residential facilities and decreases through the community to the outskirts covered by vegetation. Hazard quotients for non-cancer effects identified air-borne exposure pathways for humans to pose the largest risk, including the inhalation of vapour. The average hazard indices recorded were 0.5 (child) and 0.1 (adult) at the grid, 2 (child) and 0.3 (adult) at the transects, 1.6 (child) and 0.2 (adult) for waste soil and 76 (child) and 10.9 (adult) at the mining sites. The inhabitants of Gbani community are therefore at risk of non-cancer effects of mercury as the hazard quotients and hazard index were above one.The mechanism by which arbuscular mycorrhizal (AM) fungi immobilize lead (Pb) within the cell wall is unclear. Therefore, the aim of this study was to investigate the mechanism by which AM fungi immobilize Pb within the cell wall by measuring the Pb content in the cell wall, the polysaccharide and the uronic acid contents of different cell wall fractions, and the activity of cell wall peroxidase. Mycorrhizal-associated Medicago truncatula had higher shoot and root biomass than nonmycorrhizal-associated M. truncatula. https://www.selleckchem.com/products/-epicatechin.html AM inoculation increased the content of Pb in the cell wall under Pb stress. The polysaccharide content in the pectin and hemicellulose fractions were increased by AM inoculation with or without Pb stress. In AM-associated roots, the cell wall peroxidase activity increased in response to Pb stress. However, Pb stress did not affect the cell wall peroxidase activity in non-AM-associated roots. Correlation analysis suggested that MtPrx05 and MtPrx10 may participate in polysaccharide cross-linking and cell wall stiffening. The Pb stress resistance mechanism of AM-associated roots may involve cell wall stiffening. Taken together, the results show that AM inoculation may improve host plant growth and increase Pb immobilization in the cell wall by increasing the polysaccharide content within pectin and hemicellulose and by inducing cell wall peroxidase activity. Both the polysaccharide composition and cell wall peroxidase have important contributions to the resistance of mycorrhizal-associated plants.Here, the bio-nanocomposite (n-HFP + n-HFS)@An was developed to simultaneously immobilize Pb, Cd and As in the severely contaminated soil. The immobilization rates of diethylenetriaminepentaacetic acid (DTPA)/decarbonate-extracted bioavailable Pb, Cd and As were 59.87%, 31.28% and 62.30%, and the immobilization rates of their water-soluble forms were 63.12%, 60.02% and 89.39%, respectively. Moreover, the ten-year acid rain simulated leaching assay showed that the maximum cumulative release contents of Pb, Cd and As in the treated soil samples were decreased by 2.94, 2.46 and 40.60 times, comparing to the un-treated ones. Additionally, the results of SBRC (Solubility Bioaccessibility Research Consortium) revealed that the bioaccessible rates of the three metals in intestinal phase were lower than in gastric phase, and both of them decreased with increasing the immobilization time. The gastric bioaccessibility of Pb, Cd and As had a higher correlation with the contents of water-soluble forms, while the intestinal bioaccessibility was more strongly positively associated with the bioavailable forms.A new spectrophotometric method for measuring permanganate index (chemical oxygen demand using potassium permanganate (KMnO4) as oxidant, CODMn) in water was established. The method was based on the rapid oxidation of N,N-diethyl-p-phenylenediamine (DPD) by residual KMnO4 in digestion solution under neutral pH condition to form the stable pink radical (DPD●+). Only 20 s were enough to form the pink DPD●+. The generated DPD●+ could be quantitatively measured by a visible spectrophotometer at 551 nm. Stoichiometric coefficient of the reaction between KMnO4 and DPD was close to 15 (15.07). There was a well linear relationship (R2 = 0.999) between the change of the absorbance of DPD●+ at 551 nm and the concentration of CODMn in the range of 0-4.46 mg L-1. Limit of detection of the DPD method was as low as 0.02 mg L-1 CODMn. The DPD method was highly accurate for measuring CODMn in standard solutions with well recovery rates of 99.17%-102.22%, and was well tolerant to the interference of coexistent Cl- and Fe3+. The DPD method was successfully applied for measuring CODMn in real water samples, including surface water, underground water and drinking water.