Sex steroids administration in meat producing animals is forbidden within the EU to preserve consumers' safety, but continuous monitoring to identify resurgence of their misuse is needed. Among biomarkers related to sex steroids abuse in veal calves the regucalcin (RGN) mRNA perturbations in testis have been described in RNA samples. To setup novel diagnostic method, to update current tests available in National Residue Control Plans (NRCPs) and in legal dispute when illicit practices on farm animals are suspected, the reliability of RGN profiling was assessed by histological and molecular techniques. Formalin fixed paraffin embedded (FFPE) testis samples, chosen being the most effective preservation strategy adopted by histological NRCPs and allowing easier retrospective analysis if required by legal disputes, were analyzed from veal calves treated with nandrolone, 17β-estradiol and a cocktail of the two hormones. RGN levels were determined by quantitative Real Time PCR and Immunohistochemistry assays. Test performances were assessed and compared by multiple ROC curves. Both tests resulted sensitive and specific, allowing to enrich, in future field investigation, novel integrated diagnostic protocols needed to unveil sex steroid abuse. Developed RT-qPCR and IHC methods confirmed RGN as a useful and robust biomarker to detect illegal administration of sex steroid hormones in veal calves. The developed methods, successfully applied to ten years old FFPE blocks, could allow both retrospective analysis, when supplementary investigations are requested by authorities, and future implementation of current NRCPs. Developed RT-qPCR and IHC methods confirmed RGN as a useful and robust biomarker to detect illegal administration of sex steroid hormones in veal calves. The developed methods, successfully applied to ten years old FFPE blocks, could allow both retrospective analysis, when supplementary investigations are requested by authorities, and future implementation of current NRCPs.Rendzic Leptosols are intrazonal soils formed on limestone bedrock. https://www.selleckchem.com/products/BafilomycinA1.html The specialty of these soils is that parent rock material is more influential in shaping soil characteristics than zonal factors such as climate, especially during soil formation. Unlike fast evolving Podzols due to their leaching regime, Leptosols do not undergo rapid development due to the nature of the limestone. Little is known how microbiome reflects this process, so we assessed microbiome composition of Rendzic Leptosols of different ages, arising from disruption and subsequent reclamation. The mountains and foothills that cover much of the Crimean Peninsula are ideal for this type of study, as the soils were formed on limestone and have been subjected to anthropogenic impacts through much of human history. Microbiomes of four soil sites forming a chronosequence, including different soil horizons, were studied using sequencing of 16S rRNA gene libraries and quantitative PCR. Dominant phyla for all soil sites were Actinobacteria, Proteoboil, especially by weighted metrics. CCA analysis, in combination with PERMANOVA, linked differences in microbiomes to the joint change of all soil chemical parameters between soil horizons. Local factors, such as parent material and plant litter, more strongly influenced the microbiome composition in Rendzic Leptosols than soil age.To date, most data regarding the crosstalk between the Wnt signaling pathway and the YAP/TAZ transcriptional coactivators focuses on the Wnt/β-catenin branch of the pathway. In contrast, the relationship between the non-canonical Wnt pathway and YAP/TAZ remains significantly less explored. Wnt5a is usually regarded as a prototypical non-canonical Wnt ligand, and its expression has been related to cancer progression. On the other hand, YAP/TAZ transcriptional coactivators act in concert with TEAD transcription factors to control gene expression. Although one article has shown previously that WNT5A is a YAP/TEAD target gene, there is a need for further evidence supporting this regulatory relationship, because a possible YAP/Wnt5a regulatory circuit might have profound implications for cancer biology. This article analyzes publicly available ChIP-Seq, gene expression, and protein expression data to explore this relationship, and shows that WNT5A might be a YAP/TEAD target gene in several contexts. Moreover, Wnt5a and YAP expression are significantly correlated in specific cancer types, suggesting that the crosstalk between YAP/TAZ and the Wnt pathway is more intricate than previously thought.The dicynodont fossils from the Naobaogou Formation of Nei Mongol, China are abundant and diverse but poorly studied. In this article, one nearly complete skeleton and four cranial specimens from the Naobaogou Formation are referred to the dicynodontoid genus Turfanodon as a new species, T. jiufengensis. Previously, Turfanodon was known only from upper Permian sites in Xinjiang and Gansu. The new specimens are referred to Turfanodon based on the following characters snout tall with steeply sloping profile, anterior tip of the snout squared off, facial region heavily pitted, nasal bosses present as paired swellings near the posterodorsal margin of the external nares, preparietal depressed, intertemporal bar long and narrow, premaxilla contacting frontal, palatal surface of premaxilla exposed in lateral view, and anterior pterygoid keel restricted to the anterior tip of the anterior ramus of the pterygoid. Turfanodon jiufengensis is differentiated from the type species, T. bogdaensis, by a contact of the lacrimal with the septomaxilla, discrete, raised nasal bosses, the dorsal edge of the erupted portion of the canine tusk slightly posterior to the anterior orbital margin, an anterior extension of the lacrimal distinctly shorter than that of the prefrontal, and a premaxillary dorsal surface with a median ridge. The holotype skeleton of T. jiufengensis includes a complete axial column with 50 vertebrae (six cervical, 23 dorsal, six sacral, and 15 caudal). Turfanodon represents the first confirmed tetrapod genus shared by the late Permian faunas of the Junggar and Ordos basins, and appears to be the first dicynodont genus distributed across both tropical and temperate zones (based on paleoclimate reconstructions). Based on tetrapod fossil content, the Naobaogou Formation can be roughly correlated to the Daptocephalus Assemblage Zone of South Africa (255-252 Ma in age).