In addition, the prepared antibodies not only detected intact sericin molecules, but also detected degraded sericin that was dissolved in five different solvents. In summary, this work prepared effective sericin antibodies for silk protein synthesis and secretion research and provides a possible molecular detection method for biological products containing silkworm sericin.A novel pH-sensitive semi-interpenetrating polymer network (semi-IPN) hydrogel was prepared by using konjac glucomannan (KGM) and poly (γ-glutamic acid) (γ-PGA) with sodium trimetaphosphate (STMP) as the crosslinking agent. The structure of the semi-IPN hydrogels was characterized by FTIR spectra, thermogravimetric analysis (TGA), X-ray diffraction (XRD), rheological measurements, and scanning electron microscopy (SEM). The pH-sensitive effects were investigated by calculating the equilibrium swelling ratio (ESR) in buffer solutions (pH 2, 4, 6, and 8, respectively) at 37 °C. These results showed that the content of cross-linker and γ-PGA has a significant influence on the hydrogels' structure and swelling behavior. In vitro drug release behavior of semi-IPN hydrogels was investigated under simulated gastric and intestinal fluids using model drug Nicotinamide (NTM), and various models were applied to describe the drug release behaviors. The obtained results indicated that our synthesized semi-IPN hydrogel had the potential to be used as a suitable biomaterial carrier for functional components or drug delivery in the intestine.Corneal injury due to ocular trauma or infection is one of the most challenging vision impairing pathologies. The aim of the work was to study the effect of biodegradable silk fibroin-based scaffolds containing GDNF on the corneal regeneration process. During cultivate the highest keratocytes proliferative activity was registered with scaffolds containing 250 ng/ml and 500 ng/ml GDNF. In mice with an experimental model of epithelial-stromal damage to the cornea, silk fibroin-based scaffolds containing GDNF in various concentrations were used (in groups 1, 2 and 3 silk fibroin-based scaffolds containing GDNF in a concentration of 50 ng/ml, 250 ng/ml and 500 ng/ml, respectively; in group 4 - silk fibroin-based scaffolds without GDNF; in group 5 - a solution of GDNF with concentration of 500 ng/ml; group 6- control). The area of the corneal epithelial defect in groups 2, 3, and 5 was less than in the other groups. The most pronounced positive immunohistochemical reaction with antibodies to Bcl2, Bax, phosphoERK1/2 and phospho-JNK1/2, Ki67, Gap43 was observed in groups 2 and 3. Thus, silk fibroin-based scaffolds with GDNF stimulate the epithelialization process, proliferative activity of epithelial cells and keratocytes, accelerate the formation of the stromal nerve plexus and exhibit anti-apoptotic activity.With increasing interest in aging and skin care, the use of fillers to increase the volume of soft tissue volume is increasing globally. However, the side effects caused by the residual chemical crosslinking agents present in these fillers limit the effective application of commercialized filler products. Therefore, the development of a novel crosslinking system with a non-toxic chemical crosslinking agent is required to overcome the limitations of commercial hyaluronate (HA)-based fillers. In this paper, a new injectable hydrogel with enhanced mechanical properties, tissue adhesion, injectability, and biocompatibility is reported. The HA derivatives modified with catechol groups (HA-DA) were crosslinked by self-oxidation under in vivo physiological conditions (pH 7.4) without chemical crosslinkers to form hydrogels, which can be further accelerated by the dissolved oxygen in the body. The fabricated HA-DA filler showed excellent mechanical properties and could be easily injected with a low injection force. Further, the HA-DA filler stably attached to the injection site due to the tissue adhesion properties of the catechol groups, thus leading to an improved displacement stability. In addition, the HA-DA filler showed excellent cell viability, cell proliferation, and biocompatibility. Therefore, the HA-DA hydrogel is a novel soft tissue filler with great potential to overcome the limitations of commercial soft tissue fillers.Novel polyethyleneimine functionalized cellulose nanofiber magnetic composites (PEI-CNFs@Fe3O4) were prepared using banana peels as the raw materials for the sorption of Ni(II), Cu(II) and Cd(II) in single-component and multi-component systems. The batch experiments, spectral analyses and model fittings were used to reveal the sorption properties. The sorption of Ni(II), Cu(II) and Cd(II) on PEI-CNFs@Fe3O4 all conformed to the Langmuir isotherm and pseudo-second-order kinetic models. And the maximum sorption capacities of PEI-CNFs@Fe3O4 towards Ni(II), Cu(II) and Cd(II) were 134.38, 93.71 and 173.56 mg g-1, respectively. https://www.selleckchem.com/products/daratumumab.html The main sorption mechanism of Ni(II), Cu(II) and Cd(II) on PEI-CNFs@Fe3O4 is the strong surface complexation of the amino, carboxyl and hydroxyl groups with Ni(II), Cu(II) and Cd(II) ions. Especially, the introduction of PEI contributed to the improvement in the sorption capacities of PEI-CNFs@Fe3O4 towards the heavy metals. Besides, the size of the ionic radius and the strength of the surface complexing ability with PEI-CNFs@Fe3O4 are the reasons for the difference in the sorption capacities of Ni(II), Cu(II) and Cd(II) (Cd(II) > Ni(II) > Cu(II)). In conclusion, PEI-CNFs@Fe3O4 has shown the advantages of low cost, simple preparation, easy magnetic separation, environmental friendliness and high sorption capacity, thus having a broad application prospect in the treatment of multi-heavy metals polluted water.Chemically, chitosan is a linear polysaccharide constituted of arbitrarily distributed D-glucosamine and N-acetyl-D-glucosamine constituents combined together via β-1,4-glycosidic linkage. Because of increasing ecological awareness and strict environmental regulations, species of natural and biological origin such as chitosan can be identified as ideal environmental sustainable alternative to replace traditional heterocyclic (toxic) corrosion inhibitors. Although, chitosan contains numerous electron rich sites however chitosan itself is not highly effective aqueous phase corrosion inhibitors. Aqueous phase application of chitosan is limited because of its limited solubility. However, chemically modified chitosan derivatives, such as chitosan based Schiff bases (CSBs) exhibit remarkable solubility in such electrolytes. Therefore, recently various reports dealing with the anticorrosion potential of CSBs have been reported. Present review article describes the collections on CSBs as aqueous phase corrosion inhibitors.