, Retama sphaerocarpa L., Phillyrea angustifolia L. and Quercus ilex canopies and in open interspace. Unburned soils located underneath P. angustifolia and Q. ilex canopies were significantly more fertile than in open interspaces. The microsite effect on soil fertility was fire frequency dependent. As fire frequency increased, the plant canopy microsite effect decreased for soil organic matter (SOM), cation exchange capacity (CEC), total C, P, Ca, K and Mg, labile phosphate, arylsulfatase and acid phosphatase activities. Total N, ammonium, nitrate and β-glucosidase activity decreased with increasing fire frequency, but their spatial variability was maintained along all fire frequency scenarios. Fire frequency decreased foliar N concentration but increased P concentration in some species, leading to a decrease in their NP ratio. Our findings suggest that soil fertility heterogeneity will be reduced with increasing fire frequency. This could compromise the recovery of soil and ecosystem functioning.The effects of volcanic disturbance on aquatic communities and their recovery are poorly studied. To fill this gap, we explored the effects on fish communities in rivers in Argentina of the 2008 eruption of Chaitén Volcano in southern Chile (42.8° lat. S). The eruption produced volcanic plumes of ash that persisted in the atmosphere for several months. Borne on westerly winds, deposits of tephra crossed the Andes Mountains, reaching the Atlantic coast (Argentina). We compared the pre- and post-eruption abundances of a native catfish Hatcheria macraei, and two introduced trout from rivers covered by the volcanic plumes (Argentina) using Before-After-Control-Impact analysis to explore fish recovery. Total suspended solids from volcanic ashfall, macroinvertebrate abundance and richness, and species ecological attributes influenced the spatial arrangement of fish in rivers. Twenty-one months after the eruption, Rainbow Trout, Oncorhynchus mykiss, had not returned to pre-eruption abundances in the sampled rivers, lity.Xylella fastidiosa is one of the most destructive plant pathogenic bacteria worldwide, affecting more than 500 plant species. In Apulia region (southeastern Italy), X. fastidiosa subsp. pauca (Xfp) is responsible for a severe disease, the olive quick decline syndrome (OQDS), spreading epidemically and with dramatic impact on the agriculture, the landscape, the tourism, and the cultural heritage of this region. https://www.selleckchem.com/products/c-178.html An early detection of the infected plants would hinder the rapid spread of the disease. The main objective of this paper was to define a geostatistical approach of data fusion, which combines remote (radiometric), and proximal (geophysical) sensor data and visual inspections with plant diagnostic tests, to provide probabilistic maps of Xfp infection risk. The study site was an olive grove located at Oria (province of Brindisi, Italy), where at the time of monitoring (September 2017) only few plants showed initial symptoms of the disease. The measurements included 1) acquisitions of reflected electromagnetic radiation with UAV (Unmanned Aerial Vehicle) equipped with a multi-spectral camera; 2) geophysical surveys on the trunks of 49 plants with Ground Penetrating Radar (GPR); 3) disease severity rating, by visual inspection of the proportion of canopy with symptoms; 4) qPCR (real time-quantitative Polymerase Chain Reaction) data from tests on 61 plants. The data were submitted to a set of processing techniques to define a "data fusion" procedure, based on non-parametric multivariate geostatistics. The approach allowed marking those areas where the risk of infection was higher, and identifying the possible infection entry routes into the field. The probability map of infection risk could be used as an effective tool for a preventive action and for a better organization of the monitoring plans.Soluble and total extractable concentrations used for predicting contaminants' environmental fate may lead to uncertainties due to the lack of understanding of soil-contaminants interactions. The present study focuses on the influence of a controlled electric field on the distribution of polycyclic aromatic hydrocarbons in soil samples evaluated through a speciation scheme. Soil samples were spiked with 25,000 mg (hexadecane, phenanthrene, and pyrene 10011 w/w) per kg of soil, and speciation of hydrocarbons was determined by employing a novel Sequential Solvent Extraction procedure, resulting in five fractions soluble, pseudosoluble, desorbable, extractable, and sequestered. The distribution of hydrocarbons was then changed through the application of an electric field (72 h, 0.708 mA cm-2, 2.95 ± 0.13 V cm-1), which modified the interactions in the soil-water interface. The electrochemical treatment significantly increased the pyrene soluble, desorbable and sequestered fractions by 340, 1.3 and 19-fold (p less then 0.05); the hexadecane soluble fraction increased in 6-fold (p less then 0.05) and the phenanthrene desorbable fraction increased in 1.3-fold (p less then 0.05). The use of the speciation scheme proposed in this study provides a wider view of hydrocarbons distribution in soils, rather than using water-soluble or total extractable concentrations. Finally, this speciation scheme is proposed as a tool to evaluate the environmental fate of organic contaminants in soils.The formation and fallout of oil-related marine snow have been associated with interactions between dispersed oil and small marine particles, like phytoplankton and mineral particles. In these studies, the influences of phytoplankton species, mineral particle concentration, and oil concentration on the aggregation of oil in seawater (SW) were investigated. The experiments were performed in a low-turbidity carousel incubation system, using natural SW at 13 °C. Aggregation was measured by silhouette camera analyses, and oil compound group distribution and depletion by gas chromatography (GC-FID or GC-MS). Aggregates with median sizes larger than 500 μm in diameter were measured in the presence of dispersed oil and the phytoplankton species Thalassiosira rotula, Phaeocystis globosa, Skeletonema pseudocostatum, but not with the microalgae Micromonas pusilla. When mineral particles (diatomaceous earth) were incubated at different concentrations (5-30 mg/L) with dispersed oil and S. pseudocostatum, the largest aggregates were measured at the lower mineral particle concentration (5 mg/L).
, Retama sphaerocarpa L., Phillyrea angustifolia L. and Quercus ilex canopies and in open interspace. Unburned soils located underneath P. angustifolia and Q. ilex canopies were significantly more fertile than in open interspaces. The microsite effect on soil fertility was fire frequency dependent. As fire frequency increased, the plant canopy microsite effect decreased for soil organic matter (SOM), cation exchange capacity (CEC), total C, P, Ca, K and Mg, labile phosphate, arylsulfatase and acid phosphatase activities. Total N, ammonium, nitrate and β-glucosidase activity decreased with increasing fire frequency, but their spatial variability was maintained along all fire frequency scenarios. Fire frequency decreased foliar N concentration but increased P concentration in some species, leading to a decrease in their NP ratio. Our findings suggest that soil fertility heterogeneity will be reduced with increasing fire frequency. This could compromise the recovery of soil and ecosystem functioning.The effects of volcanic disturbance on aquatic communities and their recovery are poorly studied. To fill this gap, we explored the effects on fish communities in rivers in Argentina of the 2008 eruption of Chaitén Volcano in southern Chile (42.8° lat. S). The eruption produced volcanic plumes of ash that persisted in the atmosphere for several months. Borne on westerly winds, deposits of tephra crossed the Andes Mountains, reaching the Atlantic coast (Argentina). We compared the pre- and post-eruption abundances of a native catfish Hatcheria macraei, and two introduced trout from rivers covered by the volcanic plumes (Argentina) using Before-After-Control-Impact analysis to explore fish recovery. Total suspended solids from volcanic ashfall, macroinvertebrate abundance and richness, and species ecological attributes influenced the spatial arrangement of fish in rivers. Twenty-one months after the eruption, Rainbow Trout, Oncorhynchus mykiss, had not returned to pre-eruption abundances in the sampled rivers, lity.Xylella fastidiosa is one of the most destructive plant pathogenic bacteria worldwide, affecting more than 500 plant species. In Apulia region (southeastern Italy), X. fastidiosa subsp. pauca (Xfp) is responsible for a severe disease, the olive quick decline syndrome (OQDS), spreading epidemically and with dramatic impact on the agriculture, the landscape, the tourism, and the cultural heritage of this region. https://www.selleckchem.com/products/c-178.html An early detection of the infected plants would hinder the rapid spread of the disease. The main objective of this paper was to define a geostatistical approach of data fusion, which combines remote (radiometric), and proximal (geophysical) sensor data and visual inspections with plant diagnostic tests, to provide probabilistic maps of Xfp infection risk. The study site was an olive grove located at Oria (province of Brindisi, Italy), where at the time of monitoring (September 2017) only few plants showed initial symptoms of the disease. The measurements included 1) acquisitions of reflected electromagnetic radiation with UAV (Unmanned Aerial Vehicle) equipped with a multi-spectral camera; 2) geophysical surveys on the trunks of 49 plants with Ground Penetrating Radar (GPR); 3) disease severity rating, by visual inspection of the proportion of canopy with symptoms; 4) qPCR (real time-quantitative Polymerase Chain Reaction) data from tests on 61 plants. The data were submitted to a set of processing techniques to define a "data fusion" procedure, based on non-parametric multivariate geostatistics. The approach allowed marking those areas where the risk of infection was higher, and identifying the possible infection entry routes into the field. The probability map of infection risk could be used as an effective tool for a preventive action and for a better organization of the monitoring plans.Soluble and total extractable concentrations used for predicting contaminants' environmental fate may lead to uncertainties due to the lack of understanding of soil-contaminants interactions. The present study focuses on the influence of a controlled electric field on the distribution of polycyclic aromatic hydrocarbons in soil samples evaluated through a speciation scheme. Soil samples were spiked with 25,000 mg (hexadecane, phenanthrene, and pyrene 10011 w/w) per kg of soil, and speciation of hydrocarbons was determined by employing a novel Sequential Solvent Extraction procedure, resulting in five fractions soluble, pseudosoluble, desorbable, extractable, and sequestered. The distribution of hydrocarbons was then changed through the application of an electric field (72 h, 0.708 mA cm-2, 2.95 ± 0.13 V cm-1), which modified the interactions in the soil-water interface. The electrochemical treatment significantly increased the pyrene soluble, desorbable and sequestered fractions by 340, 1.3 and 19-fold (p less then 0.05); the hexadecane soluble fraction increased in 6-fold (p less then 0.05) and the phenanthrene desorbable fraction increased in 1.3-fold (p less then 0.05). The use of the speciation scheme proposed in this study provides a wider view of hydrocarbons distribution in soils, rather than using water-soluble or total extractable concentrations. Finally, this speciation scheme is proposed as a tool to evaluate the environmental fate of organic contaminants in soils.The formation and fallout of oil-related marine snow have been associated with interactions between dispersed oil and small marine particles, like phytoplankton and mineral particles. In these studies, the influences of phytoplankton species, mineral particle concentration, and oil concentration on the aggregation of oil in seawater (SW) were investigated. The experiments were performed in a low-turbidity carousel incubation system, using natural SW at 13 °C. Aggregation was measured by silhouette camera analyses, and oil compound group distribution and depletion by gas chromatography (GC-FID or GC-MS). Aggregates with median sizes larger than 500 μm in diameter were measured in the presence of dispersed oil and the phytoplankton species Thalassiosira rotula, Phaeocystis globosa, Skeletonema pseudocostatum, but not with the microalgae Micromonas pusilla. When mineral particles (diatomaceous earth) were incubated at different concentrations (5-30 mg/L) with dispersed oil and S. pseudocostatum, the largest aggregates were measured at the lower mineral particle concentration (5 mg/L).
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