Glutaredoxins (GRXs) are disulfide oxidoreductases that are involved in various biological processes. However, little information on the role of GRXs in the regulation of fruit ripening and the response to stress is available. In this study, we isolated 64 GRX genes from banana genome. Their encoded GRX proteins could be classified into four classes CC, CGFS, CPYC and GRL types. The distribution and synteny of these GRXs on chromosomes, the gene structures, the promoter sequences, and the possible protein subcellular localizations were characterized. Molecular interaction network analysis suggested that MaGRX might interact with glutathione reductase (GR), sulfiredoxin, peroxiredoxin (Prx), and NADPH-dependent thioredoxin reductase C (NTRC), contributing to the antioxidative defense of banana fruit. MicroRNA prediction showed that MaGRX genes might be targeted by different miRNAs. https://www.selleckchem.com/products/srt2104-gsk2245840.html Transcriptome analysis characterized the expression profiles of different MaGRX genes during banana fruit ripening, and in response to different storage stresses. The results suggested that CC-type, CPYC-type and GRL-type MaGRXs might be more active than CGFS-type MaGRXs during banana fruit ripening and the response to stress. Moreover, MaGRX6/7/9/11/17/23/28 and MaGRL3/16/19 might play important roles in regulating fruit ripening or in response to low and high temperature, or Fusarium proliferatum infection.Modified biopolymer chitosan namely 2-hydroxy-1-naphthaldehyde chitosan (CTS-Nap) has been synthesized for the removal of toxic chromium from aqueous solutions. In an attempt to enhance the adsorption capacity of toxic chromium on the prepared modified biopolymer, magnetic Fe3O4 nanoparticles have been loaded on the modified adsorbent to form the magnetite adsorbent (Fe3O4@CTS-Nap). The adsorption mechanism of both adsorbents is explored by batch experiments, FT-IR, SEM, TEM, XRD, VSM, and EDS. The optimum adsorption is achieved at pH 1.5 for CTS-Nap and 1.0 for Fe3O4@CTS-Nap. Pseudo second order illustrated the best description for the adsorption process with correlation coefficient R2 = 0.999 and the film diffusion or chemisorption is the rate-limiting step. The equilibrium data is analyzed using five isotherm models, the experimental data agreed well with the Freundlich model with a maximum adsorption capacity of 78.12 mg g-1 and 57.14 mg g-1 for CTS-Nap and Fe3O4@CTS-Nap, respectively. However, this unexpected result revealed that the presence of magnetic nanoparticles does not always enhance the adsorption process and many other factors could control the adsorption process. Generally, these outcomes revealed that the unmagnetite modified adsorbent CTS-Nap have practical greater influence on wastewater treatment management rather than the magnetic modified chitosan Fe3O4@CTS-Nap.Liver kinase B1 (LKB1) is a member of the serine/threonine kinase family, which plays an indispensable role in the organism of animals. In the current study, the chicken LKB1 protein gene was amplified by PCR based on the primers and cDNA templates. Then, the cloning vector was constructed and the target gene was cloned. After that, the target gene was inserted into the expression vector to construct the recombinant plasmid. The recombinant plasmid was transformed into BL21 (DE3) host cells and the LKB1 recombinant proteins were successfully expressed by using Isopropyl-β-D-thiogalactopyranoside (IPTG). Finally, purified LKB1 proteins were used as antigen and the rabbit-derived antiserums were collected. The antiserum titer determined by ELISA was not less than 1128000. The results of Western blot suggested that the polyclonal antibody is highly specific to chicken LKB1 protein. Immunofluorescence indicated that the LKB1 protein is mainly expressed in the cytoplasm of liver, heart and hypothalamus cells of chicken. Our study showed that the LKB1 polyclonal antibodies produced by this method are effective and can be used to further study the role of LKB1 in the pathogenesis of chicken disease.A novel antimicrobial gel composed of κ-carrageenan (KC) and a cinnamon essential oil (CEO)/hydroxypropyl-β-cyclodextrin (HPCD) composite was developed. The CEO/HPCD composite was characterized by UV-visible spectrophotometry and Fourier-transform infrared spectroscopy (FT-IR), and the changes in the principal components of CEO upon encapsulation by HPCD were analyzed by gas chromatography-mass spectrometry (GC-MS). The physicochemical properties, release kinetics and antimicrobial activity of the fabricated gels were investigated. The hardness of the KC gels increased with composite concentration in the range of 1.0-3.0% (w/v) and thereafter decreased. A similar trend was observed for the gumminess and chewiness, whereas the gel springiness remained essentially constant. The CEO/HPCD composite also enhanced the fluidity of the system, and the syneresis was positively correlated with the composite concentration. The controlled release of CEO from the gels was affected by the relative humidity (RH) and CEO content. The Ritger-Peppas model indicated that the CEO release kinetics from the gels proceeded through a combination of diffusion and framework erosion. The KC gel containing 5% CEO/HPCD composite displayed effective antimicrobial activity, prolonging the shelf life of sliced bread by at least two days. The reported gels may have potential applications as a promising material for food preservation.Sugarcane bagasse is a rich source of cellulose (32-45%), hemicellulose (20-32%) and lignin (17-32%), 1.0-9.0% ash and some extractives. Huge amount of the generation of sugarcane bagasse has been a great challenge to industries and environment at global level for many years. Though cellulosic and hemicellulosic fractions in bagasse makes it a potential raw substrate for the production of value-added products at large scale, the presence of lignin hampers its saccharification which further leads to low yields of the value-added products. Therefore, an appropriate pretreatment strategy is of utmost importance that effectively solubilizes the lignin that exposes cellulose and hemicellulose for enzymatic action. Pretreatment also reduces the biomass recalcitrance i.e., cellulose crystallinity, structural complexity of cell wall and lignification for its effective utilization in biorefinery. Sugarcane bagasse served as nutrient medium for the cultivation of diverse microorganisms for the production of industrially important metabolites including enzymes, reducing sugars, prebiotic, organic acids and biofuels.