Two meat-type broiler strains, strain A and strain B, were reared in floor pens (25 birds/pen; 45 pens/strain) for pectoralis (P) major collagen and mixed muscle protein turnover (PT) study from 0-56 D using primary breeder nutrition and husbandry guidelines. Forty broilers (n = 10/strain for collagen PT; n = 10/strain for mixed muscle PT) were selected at each sampling age at day 21, 28, 35, 42, and 56 and infused with 1-13C proline (Pro) and 15N-phenylalanine (Phe) which are used as amino acid tracers for collagen and mixed muscle PT measurements, respectively. Muscle and plasma samples were collected, and enrichments of 1-13C Pro and 15N-Phe were determined using mass spectrometry. Fractional synthesis rate (FSR) and fractional degradation rate (FDR) were measured for collagen and mixed muscle using precursor-product principle. At day 42, after separating the sampled broilers as myopathy (woody breast [WB] score > 1) and nonmyopathy (WB = 0), plasma metabolites were screened for differential 3-methyhistidih connective tissue, mainly collagen, was an evident pathophysiological phenomenon occurring in myopathy-affected broilers.Caged layer osteoporosis (CLO) is a common bone metabolism diseases and poses a great threat to the production of laying hens. So far, there is no effective nutrition intervention to prevent CLO. The objective of this study was to evaluate the effects of dietary total flavonoids from Rhizoma Drynariae (TFRD), a Chinese herbal, on bone health, egg quality, and serum antioxidant capacity of caged laying hens. A total of two hundred sixteen, 54-wk-old Lohmann Pink-shell laying hens at were allocated to 3 groups with 6 replicates of 12 hens per replicate. The control group was fed a basal diet (BD) and 2 treatment groups additionally supplied with 0.5 or 2.0 g/kg TFRD, respectively. https://www.selleckchem.com/products/Sodium-butyrate.html Results showed that supplying 2.0 g/kg TFRD enhanced the activities of serum total antioxidant capacity (P less then 0.01) and glutathione peroxidase (P less then 0.05) and had higher femur and tibia bone mineral density (both P less then 0.05) compared with the control group. Dietary 2.0 g/kg TFRD also reduced the activities of serum alkaline phosphatase (P less then 0.01), tartrate resistant acid phosphatase (P less then 0.01), and the contents of osteocalcin (P less then 0.01). Furthermore, tibia histomorphology observation showed that the microstructure of bone tissue was improved after TFRD treatment. Egg quality was not affected by TFRD while the egg weight significantly increased (P less then 0.01). These findings suggested that TFRD has beneficial effects on bone health in older caged laying hens.Two experiments were conducted to examine the effects of conditioning temperature (CT) and the interactive influence of feed acidification (FA) and CT on the performance, coefficient of apparent ileal digestibility (CAID) of nitrogen (N), starch, fat calcium (Ca) and phosphorus (P), and AME in broilers. In both experiments, each treatment was randomly allocated to 6 cages (8 birds per cage) and fed from 1 to 21 D posthatch. In experiment 1, the effect of CT was evaluated using a wheat-based diet at 3 CT unconditioned, conditioned at 60°C or 90°C. All the diets by-passed the pellet press and collected in mash form. Birds fed the diet conditioned at 90°C consumed more (P less then 0.05) feed and tended (P = 0.087) to have higher feed per gain (FG) than those fed the unconditioned diet but similar to those fed the diet conditioned at 60°C. A tendency was noted for CT to affect the CAID of N (P = 0.071) and starch (P = 0.093), with reduced digestibility values in the diet conditioned at 90°C. Conditioning at 90°C resulted in lower (P less then 0.05) AME. In experiment 2, three inclusions of an acidifier (0.0, 7.0, and 10 g/kg) and 2 CT of 60°C and 90°C were evaluated in a 3 × 2 factorial arrangement of treatments using pelleted diets. Neither the main effects nor the interaction between acidifier addition and CT was significant for weight gain, feed intake, and FG. The FA increased (P less then 0.05) the CAID of N, fat, and P at both inclusion levels and of starch at 10 g/kg. Conditioning at 90°C reduced (P less then 0.05) the CAID of starch, fat, and Ca, regardless of FA level. Overall, the present data showed that the application of high CT for broiler feed manufacture can impair nutrient utilization and, consequently the feed efficiency in broilers. Feed acidification imparts some benefits to nutrient digestibility in broilers fed pelleted wheat-based diets.This study evaluated the effects of total sulfur amino acid (TSAA) levels on the performance and intestinal health of broilers challenged with Eimeria spp. A total of 432 one-day-old off-sex Cobb 500 male chicks were randomly assigned to a 3 × 2 factorial arrangement (6 replicates/12 birds), with diets and Eimeria challenge as the main factors. The diets were as follows 70% (no methionine [Met] supplementation), 85, and 100% TSAA, supplemented with L-Met. At day 14, the challenged birds (n = 216) were orally gavaged with a pool of Eimeria acervulina, Eimeria maxima, and Eimeria tenella sporulated oocysts, and the unchallenged birds (n = 216) received water. At 6 and 12 D post inoculation (dpi), performance and intestinal health were evaluated. The challenge, regardless of diets, significantly impaired the performance, intestinal villi height, villus-to-crypt ratio, and ileal digestibility of dry matter, energy, and crude protein (CP) and modulated the tight junction protein (TJP) expression throughout the expand intestinal health in birds challenged or not challenged with Eimeria spp.Lignocellulose is a constituent of plant cell walls and might be used as a fiber source in poultry nutrition. The current study investigated the impact of increasing dietary levels of lignocellulose on performance, nutrient digestibility, excreta DM, intestinal microbiota, and bacterial metabolites in slow growing broilers. At an age of 10 wk, 60 male broilers of an intercross line (New Hampshire × White Leghorn) were allocated to cages and fed isoenergetic and isonitrogenous diets containing 0.8% (LC1), 5% (LC2), or 10% (LC3) lignocellulose. After 23 D of feeding, broilers were killed and digesta samples of ileum and excreta analyzed for nutrient digestibility and DM. Cecal contents were analyzed for microbial composition and metabolites. Broiler performance was not affected by feeding dietary lignocellulose. LC3 fed broilers showed reduced ileal digestibility of protein compared to chickens fed LC1 (P = 0.003). Moreover, increasing levels of dietary lignocellulose reduced apparent digestibility of organic matter and gross energy (P less then 0.