Recent public policies in developing countries have emerged to address challenges of delivering water-related ecosystem services in urban areas. Some initiatives, such as the Brazilian Plan for Adaptation to Climate Change (BPACC) highlights sustainable urban drainage as a key strategy for promoting sustainable cities, including ecosystem-based adaptation (EbA) measures. Despite the importance of these national guidelines, little is known about how the recommendations are incorporated and the provision of ecosystem services are perceived in local initiatives. We aim to explore stakeholders' perception of ecosystem services in relation to public urban programs for improving the local environment through EbA measures. For this, we studied a stream revitalization project in the city of São Paulo, Brazil, as an emblematic case by integrating three public programs re-urbanization of irregular settlements, implementation of linear parks and cleansing of urban streams. Our methods involved literature review, documentary data, field surveys and semi-structured interviews with local populations, public agents, and NGOs. Despite some positive results of supplying ecosystem services, we recommend that local programs expand the scope of EbA measures based on BPACC guidelines, strengthen the specific objectives of the three individual public programs and better manage public resources, especially in the context of promoting resilient cities in developing countries. At the same time, local programs can teach lessons and show opportunities for improving national guidelines on climate change adaptation.Gestagens are active ingredients in human and veterinary drugs with progestogenic activity. Two gestagens-progesterone (P4), and the synthetic P4 analogue, melengestrol acetate (MGA)-are approved for use in beef cattle agriculture in North America. Both P4 and MGA have been measured in surface water receiving runoff from animal agricultural operations. This project aimed to assess the morphometric and molecular consequences of chronic exposures to P4, MGA, and their mixture during Western clawed frog metamorphosis. Chronic exposure (from embryo to metamorphosis) to MGA (1.7 µg/L) or P4 + MGA (0.22 µg/L P4 + 1.5 µg/L MGA) caused a considerable dysregulation of metamorphic timing, as evidenced by an inhibition of growth, narrower head, and lack of forelimb emergence in all animals. Molecular analysis revealed that chronic exposure to the mixture induced an additive upregulation of neurosteroid-related (GABAA receptor subunit α6 (gabra6) and steroid 5-alpha reductase 1 (srd5α1) gene expression in brain tissue. Chronic P4 exposure (0.26 µg/L P4) induced a significant upregulation of the expression hypothalamic-pituitary-gonadal (HPG)-related genes (ipgr, erα) in the gonadal mesonephros complex (GMC). Our data suggest that exposure to P4, MGA, and their mixture induces multiple endocrine responses and adverse effects in larval Western clawed frogs. This study helps to better our understanding of the consequences of chronic gestagen exposure and suggests that the implications and risk of high gestagen use in beef cattle feeding operations may extend to the aquatic environment.Artists, using empirical knowledge, manage to generate and play with giant soap films and bubbles. Until now, scientific studies of soap films generated at a controlled velocity and without any feeding from the top, studied films of a few square centimeters. The present work aims to present a new setup to generate and characterize giant soap films (2 m [Formula see text] 0.7 m). Our setup is enclosed in a humidity-controlled box of 2.2 m high, 1 m long, and 0.75 m large. Soap films are entrained by a fishing line withdrawn out of a bubbling solution at various velocities. We measure the maximum height of the generated soap films, as well as their lifetime, thanks to automatic detection. This is allowed by light-sensitive resistors collecting the light reflected on the soap films and ensures robust statistical measurements. In the meantime, thickness measurements are performed with a UV-VIS-spectrometer, allowing us to map the soap film's thickness over time.Biofilms are complex communities of bacteria that exhibit a variety of collective behaviors. These behaviors improve their ability to survive in many different environments. One of these collective behaviors seen in Bacillus subtilis is the ability for starving cells to stop the growth of other cells using potassium signaling and voltage changes. This signaling produces an oscillatory growth pattern so that during periods of low growth the nutrients diffuse deeper into the biofilm and reach the nutrient-starved, interior regions of the biomass. In this paper, we develop a mathematical model to describe this oscillatory behavior, and we use this model to develop a two-dimensional simulation that reproduces many of the important features seen in the experimental data. This simulation allows us to examine the spatial patterning of the oscillatory behavior to better understand the relationships between the various regions of the biofilm. Studying the spatial components of the metabolic and voltage oscillations could allow for the development of new control techniques for biofilms with complex shapes.Several rapid antigen tests (RATs) for the detection of SARS-CoV-2 were evaluated recently. However, reliable performance data for laboratory-based, high-throughput antigen tests are lacking. https://www.selleckchem.com/products/vvd-214.html Therefore and in response to a short-term shortage of PCR reagents, we evaluated DiaSorin's LIAISON SARS-CoV-2 antigen test in comparison to RT-qPCR, and concerning the application of screening non-COVID-19 patients on hospital admission. Applying the manufacturer-recommended cut-off of 200 arbitrary units (AU/mL) the specificity of the LIAISON Test was 100%, the overall analytical sensitivity 40.2%. Lowering the cut-off to 100 AU/mL increased the sensitivity to 49.7% and decreased the specificity to 98.3%. Confining the analysis to samples with an RT-qPCR result  less then  25 Ct resulted in a sensitivity of 91.2%. The quality of the LIAISON test is very similar to that of good RATs described in the literature with the advantage of high throughput and the disadvantage of relatively long analysis time. It passes the WHO quality criteria for rapid antigen tests and is characterized by particularly high specificity.