carotene. Both, plasma membrane integrity and mitochondrial membrane potential measurements under acute 18-h assays showed that purified BR isomers were non-toxic to cultured human THP-1 cells. Copyright © 2020 Flores, Hoyos, Venegas, Galetovic´, Zúñiga, Fábrega, Paredes, Salazar-Ardiles, Vilo, Ascaso, Wierzchos, Souza-Egipsy, Araya, Batista-García and Gómez-Silva.Because of the remarkable efficacy in treating mycobacterial infections, rifamycin and its derivatives are still first-line antimycobacterial drugs. It has been intensely studied to increase rifamycin yield from Amycolatopsis mediterranei, and nitrate is found to provide a stable and remarkable stimulating effect on the rifamycin production, a phenomenon known as "nitrate-stimulating effect (NSE)". Although the NSE has been widely used for the industrial production of rifamycin, its detailed molecular mechanism remains ill-defined. https://www.selleckchem.com/products/rp-102124.html And our previous study has established that the global nitrogen regulator GlnR may participate in the NSE, but the underlying mechanism is still enigmatic. Here, we demonstrate that GlnR directly controls rifamycin biosynthesis in A. mediterranei and thus plays an essential role in the NSE. Firstly, GlnR specifically binds to the upstream region of rifZ, which leads us to uncover that rifZ has its own promoter. As RifZ is a pathway-specific activator for the whole rif cluster, GlnR Copyright © 2020 Liu, Liu, Lei, Zhao and Wang.The gut microbiota (GM) displays a profound ability to adapt to extrinsic factors, such as gastrointestinal pathogens and/or dietary alterations. Parasitic worms (helminths) and host-associated GM share a long co-evolutionary relationship, exerting mutually modulatory effects which may impact the health of the host. Moreover, dietary components such as prebiotic fibers (e.g. inulin) are capable of modulating microbiota toward a composition often associated with a healthier gut function. The effect of helminth infection on the host microbiota is still equivocal, and it is also unclear how parasites and prebiotic dietary components interact to influence the microbiota and host health status. Some helminths, such as Trichuris suis (porcine whipworm), also exhibit strong immunomodulatory and anti-inflammatory effects. We therefore explored the effects of T. suis, alone and in interaction with inulin, both in fecal microbiota during the infection period and luminal microbiota across four intestinal segments at the end of a 4-week infection period. We observed that T. suis generally had minimal, but mainly positive, effects on the microbiota. T. suis increased the relative abundance of bacterial genera putatively associated with gut health such as Prevotella, and decreased bacteria such as Proteobacteria that have been associated with dysbiosis. Interestingly, dietary inulin interacted with T. suis to enhance these effects, thereby modulating the microbiota toward a composition associated with reduced inflammation. Our results show that administration of T. suis together with the consumption of prebiotic inulin may have the potential to positively affect gut health. Copyright © 2020 Stolzenbach, Myhill, Andersen, Krych, Mejer, Williams, Nejsum, Stensvold, Nielsen and Thamsborg.The bioremediation of persistent organohalide molecules under anoxic conditions mostly relies on the bacterial process called organohalide respiration (OHR). Organohalide-respiring bacteria (OHRB) are phylogenetically diverse anaerobic bacteria that share the capacity to use organohalides as terminal electron acceptors in an energy-conserving process. The reductive dehalogenase (rdh) gene clusters encode for proteins specialized in the respiration of one or a limited number of organohalides. One particular OHRB may harbor up to several dozens of rdh gene clusters suggesting a wide potential for bioremediation. To avoid wasting energy in producing unnecessary proteins, rdh gene clusters often include a transcriptional regulator. In organohalide-respiring Firmicutes, RdhK is a dedicated transcriptional regulator of OHR and represents a subfamily of proteins among the CRP/FNR superfamily of regulators. RdhK proteins are composed of an effector-binding domain (EBD) which recognizes a given organohalide and subseqle Hybrid A was less specific for the DNA motif, Hybrid B successfully mimicked the binding behavior of the parental proteins and thus represents a promising template for the design of new RdhK hybrids to screen yet uncharacterized RdhK proteins and also possibly other members of the CRP/FNR superfamily. Copyright © 2020 Willemin, Vingerhoets, Holliger and Maillard.A systematic comparison study was carried out to investigate the effect of Flavobacterium sp. on AISI 1045 steel corrosion by weight loss, fluorescence microscopy (FM), surface analysis, cell count, pH measure, electrochemical impedance spectroscopy (EIS), and polarization curves. The impedances were considerably increased by Flavobacterium sp. between 1 and 7 day exposure and after 30 day exposure but considerably decreased by Flavobacterium sp. after 15 and 21 day exposure, which were supported by the Icorr results and the weight loss data. Furthermore, the biofilm was formed on the coupons. The pH values were considerably decreased by Flavobacterium sp. after 15 and 21 day exposure. The results proved that Flavobacterium sp. decreased the corrosion rates between 1 and 7 day exposure and after 30 day exposure and increased the corrosion rates between 15 and 21 day exposure, which could be ascribed to the protective biofilm and the secreted corrosive acid, respectively. In addition, Flavobacterium sp. considerably increased the pit numbers, the maximum pit depths, and the corresponding widths and considerably decreased the Epit values. Importantly, the coverage and the heterogeneity of the biofilm were positively correlated with the increases in the maximum pit depths and the corresponding widths and the decreases in the Epit values by Flavobacterium sp. The results demonstrated that Flavobacterium sp. increased the pitting corrosion, which could involve the heterogeneous biofilm cover. Copyright © 2020 Wu, Zhang, Chai and Yu.