Nowadays, three dimensional (3D) cell cultures are widely used in the biological laboratories and several optical clearing approaches have been proposed to visualize individual cells in the deepest layers of cancer multicellular spheroids. However, defining the most appropriate clearing approach for the different cell lines is an open issue due to the lack of a gold standard quantitative metric. https://www.selleckchem.com/products/thiostrepton.html In this article, we describe and share a single-cell resolution 3D image dataset of human carcinoma spheroids imaged using a light-sheet fluorescence microscope. The dataset contains 90 multicellular cancer spheroids derived from 3 cell lines (i.e. T-47D, 5-8F, and Huh-7D12) and cleared with 5 different protocols, precisely ClearT, ClearT2, CUBIC, ScaleA2, and Sucrose. To evaluate image quality and light penetration depth of the cleared 3D samples, all the spheroids have been imaged under the same experimental conditions, labelling the nuclei with the DRAQ5 stain and using a Leica SP8 Digital LightSheet microscope. The clearing quality of this dataset was annotated by 10 independent experts and thus allows microscopy users to qualitatively compare the effects of different optical clearing protocols on different cell lines. It is also an optimal testbed to quantitatively assess different computational metrics evaluating the image quality in the deepest layers of the spheroids.A set of synthetic data, of antibacterial evaluation against gram-positive bacteria, as well as, the interaction of bacterial with lipid-core nanocapsules containing fusidic acid is presented here. In this data set, the analytical data are detailed; serial microdilution; nanoparticle tracking analysis; transmission electron microscopy; minimum inhibitory concentration; diameter size and zeta potential, and infra-red of the formulations before and after contact with bacteria.The ability of bivalve mollusks to accumulate chemical elements from the environment makes them an important object for environmental monitoring and assessments of anthropogenic impact on marine ecosystems. The paper presents the data on the content of 65 chemical elements in soft tissues and shells of five species of bivalve mollusks (Astarte crenata, Ciliatocardium ciliatum, Portlandia arctica, Chlamys islandica, Hiatella arctica), as well as in the upper layer (0-5 cm) of bottom sediments of the Kara Sea, the Arctic Ocean. The content of major-, trace and rare-earth elements was determined by atomic emission and inductively coupled plasma mass spectrometry (ICP-AES; ICP-MS). The age, size, and weight of mollusks were measured. There were differences in the content of chemical elements accumulated in soft tissues and shells of mollusks, as well as the significant interspecific difference in the chemical composition of the studied mollusks. The soft tissues of mollusks were accumulated with toxic metals (Hg, Ag, and Cd) in comparison with the content of these elements in bottom sediments. In Chlamys islandica and Hiatella arctica, the shell was accumulated in Cd in contrast to other species. The data obtained may be used for a comprehensive assessment of the state of ecosystems in the seas of the Siberian sector of the Arctic Ocean under changing environment and for studying the fundamental base of the accumulation of chemical elements by marine organisms.Oxygen and hydrogen isotope data of meteoric water samples are compiled from several States across Mexico. This dataset includes 287 oxygen and hydrogen (and deuterium excess) isotope data corresponding to meteoric water collected from the surface, groundwater wells, irrigation and observation wells, and water supply boreholes. These data facilitate the development of maps to determine the spatial distribution of water stable isotopes, also known as "isoscapes", of the Mexican territory. As such, this dataset (and the isoscapes built from it) is useful in geographic mobility studies that aim to evaluate geographic origins and residency of particular human and/or non-human individuals in antiquity and in contemporary times. Further discussion about the data and an example of an isoscape of Mexico using the meteoric water oxygen isotope data are provided in "Residential Patterns of Mexica Human Sacrifices at Mexico-Tenochtitlan and Mexico-Tlatelolco Evidence from Phosphate Oxygen Isotopes" (Moreiras Reynaga et al., 2021). Overall, the dataset is useful in developing interpolated maps of water stable isotopes for relevant archeological, bioarchaeological, forensic, hydrogeological, and ecological research.Soft denture lining (SDL) are acrylic or silicone based materials that can be cured with heat or auto polymerization process, which is commonly used in removable prosthodontics to reline the intaglio (interior) surface of the denture. Loss of softness due to aging process is caused by loss of plasticizer and other soluble component. However, water sorption causes changes in structure and increased materials hardness, thus need frequent replacement of the materials. To extend the durability of the SDL, sealer coating (SC) was used. In this data, sealant coating acts as mechanical barrier to prevent water sorption and solubility of the chemical component to preserved material hardness. This article provides the data of sealant coating effect on hardness and water sorption of acrylic-based and silicone-based SDL materials was arranged in 4 test group pristine and coated acrylic SDL and pristine silicone SDL and coated silicone SDL. Shore Hardness test was carried out using a Shore A Durometer and water sorption data were presented. Accordingly, the data was statistically analyzed for comparison using independent T-test for shore A hardness and water sorption.This data article presents a rich original experimental video sources and wide collections of laboratory data on water levels, sediment depths and wave front celerity values arose from different multiphase dam-break scenarios. The required data of dam-break shock waves in highly silted-up reservoirs with various initial up- and down-stream hydraulic conditions is obtained directly from high-quality videos. The multi-layer shock waves were recorded by three professional cameras mounted along the laboratory channel. The extracted video images were rigorously scrutinized, and the datasets were obtained through the images via image processing method. Different sediment depths in the upstream reservoir and dry- or wet-bed downstream conditions were considered as initial conditions, compromising a total of 32 different scenarios. A total of 198 original experimental videos are made available online in the public repository "Mendeley Data" in 8 groups based on 8 different initial upstream sediment depths [1], [2], [3], [4], [5], [6], [7], [8].
Nowadays, three dimensional (3D) cell cultures are widely used in the biological laboratories and several optical clearing approaches have been proposed to visualize individual cells in the deepest layers of cancer multicellular spheroids. However, defining the most appropriate clearing approach for the different cell lines is an open issue due to the lack of a gold standard quantitative metric. https://www.selleckchem.com/products/thiostrepton.html In this article, we describe and share a single-cell resolution 3D image dataset of human carcinoma spheroids imaged using a light-sheet fluorescence microscope. The dataset contains 90 multicellular cancer spheroids derived from 3 cell lines (i.e. T-47D, 5-8F, and Huh-7D12) and cleared with 5 different protocols, precisely ClearT, ClearT2, CUBIC, ScaleA2, and Sucrose. To evaluate image quality and light penetration depth of the cleared 3D samples, all the spheroids have been imaged under the same experimental conditions, labelling the nuclei with the DRAQ5 stain and using a Leica SP8 Digital LightSheet microscope. The clearing quality of this dataset was annotated by 10 independent experts and thus allows microscopy users to qualitatively compare the effects of different optical clearing protocols on different cell lines. It is also an optimal testbed to quantitatively assess different computational metrics evaluating the image quality in the deepest layers of the spheroids.A set of synthetic data, of antibacterial evaluation against gram-positive bacteria, as well as, the interaction of bacterial with lipid-core nanocapsules containing fusidic acid is presented here. In this data set, the analytical data are detailed; serial microdilution; nanoparticle tracking analysis; transmission electron microscopy; minimum inhibitory concentration; diameter size and zeta potential, and infra-red of the formulations before and after contact with bacteria.The ability of bivalve mollusks to accumulate chemical elements from the environment makes them an important object for environmental monitoring and assessments of anthropogenic impact on marine ecosystems. The paper presents the data on the content of 65 chemical elements in soft tissues and shells of five species of bivalve mollusks (Astarte crenata, Ciliatocardium ciliatum, Portlandia arctica, Chlamys islandica, Hiatella arctica), as well as in the upper layer (0-5 cm) of bottom sediments of the Kara Sea, the Arctic Ocean. The content of major-, trace and rare-earth elements was determined by atomic emission and inductively coupled plasma mass spectrometry (ICP-AES; ICP-MS). The age, size, and weight of mollusks were measured. There were differences in the content of chemical elements accumulated in soft tissues and shells of mollusks, as well as the significant interspecific difference in the chemical composition of the studied mollusks. The soft tissues of mollusks were accumulated with toxic metals (Hg, Ag, and Cd) in comparison with the content of these elements in bottom sediments. In Chlamys islandica and Hiatella arctica, the shell was accumulated in Cd in contrast to other species. The data obtained may be used for a comprehensive assessment of the state of ecosystems in the seas of the Siberian sector of the Arctic Ocean under changing environment and for studying the fundamental base of the accumulation of chemical elements by marine organisms.Oxygen and hydrogen isotope data of meteoric water samples are compiled from several States across Mexico. This dataset includes 287 oxygen and hydrogen (and deuterium excess) isotope data corresponding to meteoric water collected from the surface, groundwater wells, irrigation and observation wells, and water supply boreholes. These data facilitate the development of maps to determine the spatial distribution of water stable isotopes, also known as "isoscapes", of the Mexican territory. As such, this dataset (and the isoscapes built from it) is useful in geographic mobility studies that aim to evaluate geographic origins and residency of particular human and/or non-human individuals in antiquity and in contemporary times. Further discussion about the data and an example of an isoscape of Mexico using the meteoric water oxygen isotope data are provided in "Residential Patterns of Mexica Human Sacrifices at Mexico-Tenochtitlan and Mexico-Tlatelolco Evidence from Phosphate Oxygen Isotopes" (Moreiras Reynaga et al., 2021). Overall, the dataset is useful in developing interpolated maps of water stable isotopes for relevant archeological, bioarchaeological, forensic, hydrogeological, and ecological research.Soft denture lining (SDL) are acrylic or silicone based materials that can be cured with heat or auto polymerization process, which is commonly used in removable prosthodontics to reline the intaglio (interior) surface of the denture. Loss of softness due to aging process is caused by loss of plasticizer and other soluble component. However, water sorption causes changes in structure and increased materials hardness, thus need frequent replacement of the materials. To extend the durability of the SDL, sealer coating (SC) was used. In this data, sealant coating acts as mechanical barrier to prevent water sorption and solubility of the chemical component to preserved material hardness. This article provides the data of sealant coating effect on hardness and water sorption of acrylic-based and silicone-based SDL materials was arranged in 4 test group pristine and coated acrylic SDL and pristine silicone SDL and coated silicone SDL. Shore Hardness test was carried out using a Shore A Durometer and water sorption data were presented. Accordingly, the data was statistically analyzed for comparison using independent T-test for shore A hardness and water sorption.This data article presents a rich original experimental video sources and wide collections of laboratory data on water levels, sediment depths and wave front celerity values arose from different multiphase dam-break scenarios. The required data of dam-break shock waves in highly silted-up reservoirs with various initial up- and down-stream hydraulic conditions is obtained directly from high-quality videos. The multi-layer shock waves were recorded by three professional cameras mounted along the laboratory channel. The extracted video images were rigorously scrutinized, and the datasets were obtained through the images via image processing method. Different sediment depths in the upstream reservoir and dry- or wet-bed downstream conditions were considered as initial conditions, compromising a total of 32 different scenarios. A total of 198 original experimental videos are made available online in the public repository "Mendeley Data" in 8 groups based on 8 different initial upstream sediment depths [1], [2], [3], [4], [5], [6], [7], [8].
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