Escherichia coli strain K-12 MG1655 has been proposed as an appropriate host strain for industrial production. However, the direct application of this strain suffers from the transformation inefficiency and plasmid instability. Herein, we conducted genetic modifications at a serial of loci of MG1655 genome, generating a robust and universal host strain JW128 with higher transformation efficiency and plasmid stability that can be used to efficiently produce desired chemicals after introducing the corresponding synthetic pathways. Using JW128 as the host, the titer of isobutanol reached 5.76 g/L in shake-flask fermentation, and the titer of lycopene reached 1.91 g/L in test-tube fermentation, 40-fold and 5-fold higher than that of original MG1655, respectively. These results demonstrated JW128 is a promising chassis for high-level production of value-added chemicals.[This corrects the article DOI 10.1016/j.synbio.2019.05.001.][This corrects the article DOI 10.1016/j.synbio.2020.05.002.][This corrects the article DOI 10.1016/j.synbio.2019.06.002.][This corrects the article DOI 10.1016/j.synbio.2019.08.003.][This corrects the article DOI 10.1016/j.synbio.2020.06.007.][This corrects the article DOI 10.1016/j.synbio.2019.10.002.][This corrects the article DOI 10.1016/j.synbio.2020.01.002.][This corrects the article DOI 10.1016/j.synbio.2020.08.002.][This corrects the article DOI 10.1016/j.synbio.2019.07.001.][This corrects the article DOI 10.1016/j.synbio.2019.08.001.][This corrects the article DOI 10.1016/j.synbio.2020.04.001.].[This corrects the article DOI 10.1016/j.synbio.2018.02.001.][This corrects the article DOI 10.1016/j.synbio.2020.05.001.][This corrects the article DOI 10.1016/j.synbio.2020.02.001.][This corrects the article DOI 10.1016/j.synbio.2020.05.005.][This corrects the article DOI 10.1016/j.synbio.2018.02.005.][This corrects the article DOI 10.1016/j.synbio.2020.05.006.][This corrects the article DOI 10.1016/j.synbio.2019.02.001.][This corrects the article DOI 10.1016/j.synbio.2018.02.002.][This corrects the article DOI 10.1016/j.synbio.2018.03.002.][This corrects the article DOI 10.1016/j.synbio.2018.10.007.][This corrects the article DOI 10.1016/j.synbio.2019.10.003.][This corrects the article DOI 10.1016/j.synbio.2020.06.002.][This corrects the article DOI 10.1016/j.synbio.2019.05.002.][This corrects the article DOI 10.1016/j.synbio.2020.01.005.][This corrects the article DOI 10.1016/j.synbio.2018.09.003.].[This corrects the article DOI 10.1016/j.synbio.2018.10.005.][This corrects the article DOI 10.1016/j.synbio.2018.11.002.][This corrects the article DOI 10.1016/j.synbio.2018.09.004.][This corrects the article DOI 10.1016/j.synbio.2017.12.004.][This corrects the article DOI 10.1016/j.synbio.2018.08.001.][This corrects the article DOI 10.1016/j.synbio.2018.09.001.][This corrects the article DOI 10.1016/j.synbio.2018.10.012.][This corrects the article DOI 10.1016/j.synbio.2018.11.001.][This corrects the article DOI 10.1016/j.synbio.2018.12.001.][This corrects the article DOI 10.1016/j.synbio.2019.02.002.][This corrects the article DOI 10.1016/j.synbio.2019.02.003.][This corrects the article DOI 10.1016/j.synbio.2019.01.002.][This corrects the article DOI 10.1016/j.synbio.2018.09.002.][This corrects the article DOI 10.1016/j.synbio.2018.10.006.][This corrects the article DOI 10.1016/j.synbio.2018.11.003.].Despite the infection risk associated with the consumption of contaminated food, techniques for recovering and detecting Cryptosporidium oocysts from fruit and vegetables are generally inadequate due to the variable recovery efficiencies and high reagent costs, such as those presented by ISO 187442016 "Microbiology of the food chain -Detection and enumeration of Cryptosporidium and Giardia in fresh leafy green vegetables and berry fruits". Although an improved method for recovering these parasites from Iceberg lettuce, which reported increased recovery efficiency as well as lower costs, has been published, it appears to have limitations for the recovery of Cryptosporidium from saponin-rich leaves such as spinach (Spinacia oleraceae), which have previously been implicated in Cryptosporidium parvum outbreaks. In https://www.selleckchem.com/products/PHA-739358(Danusertib).html , we refined the method to improve its use with these more challenging samples. #link# The use of alkaline elution buffer (1 M glycine) of different pH values was evaluated for their effectiveness in removing C. parvum from spinach leaves. The refinement of Utaaker's method showed, from spinach leaves inoculated with 100 oocysts, an increased oocyst recovery rate with an overall mean recovery rate of 33.79% ± 2.82%. The emergence of parasitic foodborne illnesses and outbreaks associated with the consumption of fresh produce demonstrates the need for the development of an optimal recovery process for parasites from suspected foods. Results showed that refinement of existing protocols could improve the retrieval of Cryptosporidium oocysts from these more challenging leafy greens.Babesia sp. are intracellular parasitic organisms that affects mainly the red blood cells of most mammals, causing the disease known as babesiosis, and transmitted by ticks. Babesisosis is potentially fatal and a major disease of dogs in Nigeria. Therefore, active and routine surveillance is recommended. In this study, the infection was investigated among apparently healthy domestic dogs in six Area Councils of the Federal Capital Territory (FCT), Abuja, Nigeria with the aim of determining the prevalence of the infection and the associated risk factors. Blood samples were collected from dogs (n = 480) at randomly selected households, from September 2015 to August 2016. Data regarding sampling location, sex, age, breed, use, presence or absence of ticks were recorded. Blood smears were prepared, stained with Geimsa stain, and examined under light microscope for Babesia sp. The results showed an overall prevalence of 10.8% Babesia canis infection. The prevalence among dogs examined in the six Area Councils were babesiosis in the FCT was significantly dependent on age, use of dogs, tick infestation, and season. Therefore, priorities should be given to these factors while instituting control measures against the infection.