BACKGROUND Although low-intensity pulsed ultrasound has been reported to be potential cartilage regeneration, there still unresolved treatment due to cartilage fibrosis and degeneration by a lack of rapid and high-efficiency treatment. The purpose of this study was to investigate the effect of a combination therapy of focused acoustic force and stem cells at site for fast and efficient healing on cartilage regeneration. METHODS Using a rat articular cartilage defects model, one million adipose tissue-derived stem cells (ASCs) were injected into the defect site, and low-intensity focused ultrasound (LOFUS) in the range of 100-600 mV was used for 20 min/day for 2 weeks. All experimental groups were sacrificed after 4 weeks in total. The gross appearance score and hematoxylin and eosin (H&E), Alcian blue, and Safranin O staining were used for measuring the chondrogenic potential. The cartilage characteristics were observed, and type II collagen, Sox 9, aggrecan, and type X collagen were stained with immunofluorescence. The results of the comprehensive analysis were calculated using the Mankin scoring method. RESULTS The gross appearance scores of regenerated cartilage and chondrocyte-like cells in H&E images were higher in LOFUS-treated groups compared to those in negative control or ASC-treated groups. Safranin O and Alcian blue staining demonstrated that the 100 and 300 mV LOFUS groups showed greater synthesis of glycosaminoglycan and proteoglycan. The ASC + LOFUS 300 mV group showed positive regulation of type II collagen, Sox 9 and aggrecan and negative regulation of type X collagen, which indicated the occurrence of cartilage regeneration based on the Mankin score result. CONCLUSION The combination therapy, which involved treatment with ASC and 300 mV LOFUS, quickly and effectively reduced articular cartilage defects.Salinity has a significant impact on the sewage treatment efficiency of constructed wetlands (CWs), as well as affecting the greenhouse gas emissions of CWs. A lab-scale CW simulation system was constructed to observe the treatment efficiency and greenhouse gas flux occurring in CWs at different influent salinities (0%, 0.5%, 1.0%, 1.5%, and 2.0%). The results show that (1) the removal rates of COD, TN, NH4+-N, NO3--N, and TP reach the highest at salinity of 0 or 0.5%. And the lowest removal rates are all at a salinity of 2.0%. (2) The emission flux of CO2, CH4, and N2O in CWs varies with an increase in salinity. The trends of CO2 and CH4 emission flux were consistent with those of COD reduction rate. However, it was opposite for N2O flux to that of TN, NH4+-N, and NO3--N removal rate. Affected by salinity, the greenhouse gas emission flux in this study is generally lower than what was reported in literature. (3) Correlation analysis showed that CO2 and CH4 emission fluxes were positively correlated with the COD reduction rate. https://www.selleckchem.com/products/envonalkib.html N2O emission flux was negatively correlated with the removal rates of TN, NH4+-N, and NO3--N. The results suggest that different pollutants are inhibited by salinity to different degrees. COD is more affected by salinity than nitrogen and phosphorus, while nitrogen is more easily inhibited by salinity than phosphorus. CWs can have a high removal rate of pollutants in treating low-salinity wastewater. Although increased salinity reduces treatment efficiency of wastewater to some extent, it also inhibits the emission of CO2 and CH4.A simple approach was developed for the rapid and accurate estimation of 5-day biochemical oxygen demand (BOD5) in food processing wastewater. Immobilization of the natural microbial consortium that was collected from an aerobic compartment of a food processing wastewater treatment plant was simply performed by adhesion using a low-cost porous carrier. Pseudomonas aeruginosa, Bacillus cereus, and Streptomyces, whose salt-tolerance and ability to break down organic compounds have been widely reported, were found to be predominant. These microorganisms may cause an enhancement of the bioreactor response in the presence of sodium chloride. Consequently, a modified glucose-glutamic acid (GGA) calibration standard was proposed in which an appropriate amount of NaCl was added; this solution was found to be more effective in terms of accuracy and practicality than both conventional GGA and the synthetic wastewater recipe from the Organisation for Economic Cooperation and Development (OECD). The calibrated self-built packed-bed bioreactor exhibited good precision of 3% or less in predicting BOD5 in influent, which is similar to the performance of the most common commercial biochemical oxygen demand (BOD) bioreactors. There was a statistical agreement between the results obtained from this rapid BOD biosensor and the conventional methods, even when testing treated wastewater samples.The distribution character of 41 antibiotics belonging to 6 groups, sulfonamides (SAs), quinolones (QUs), tetracyclines (TCs), macrolides (MLs), penicillins (PLs), and chloramphenicol (CHL), was investigated in drinking water sources of Nanjing during 2017-2019. MLs (42.98%) were the most abundant category, followed by SAs (25.94%) and QUs (22.52%). The dominant antibiotic was ofloxacin (OFX) in Dec. 2017 (average concentration, 3.14 ng/L; range, ND-35.20 ng/L) and Nov. 2018 (2.16 ng/L, ND-12.26 ng/L), and sulfadiazine (SDZ) in Mar. 2019 (16.37 ng/L, ND-25.90 ng/L). For Dec. 2017, the total concentrations in Zhongshan Waterworks (S15) and Jinniushan Reservoir (S16) were significantly higher than the other sampling sites, which may be attributed to point source pollution. The ecological and human risk of the main antibiotics was assessed by risk quotients (RQs) and target hazard quotient (THQ), respectively. Most of the RQ values were below 0.1, except enrofloxacin (ERX, 0.11) and enoxacin (ENX, 0.62) in Dec. 2017, lomefloxacin (LOM, 0.14) in Nov. 2018, and LOM (0.28) and ERX (0.10) in Mar. 2019. This indicated that the risk of the target antibiotics to aquatic organisms in the 3 years was moderate or low level. Meanwhile, results of the THQ values showed that antibiotic exposure caused no risk to human health. This research provides scientific information for antibiotic pollution control and enriches environmental monitoring data in the drinking water sources.