The current study aims to develop a safe and highly immunogenic COVID-19 vaccine. The novel combination of a DNA vaccine encoding the full-length Spike (S) protein of SARS-CoV-2 and a recombinant S1 protein vaccine induced high level neutralizing antibody and T cell immune responses in both small and large animal models. More significantly, the co-delivery of DNA and protein components at the same time elicited full protection against intratracheal challenge of SARS-CoV-2 viruses in immunized rhesus macaques. As both DNA and protein vaccines have been proven safe in previous human studies, and DNA vaccines are capable of eliciting germinal center B cell development, which is critical for high-affinity memory B cell responses, the DNA and protein co-delivery vaccine approach has great potential to serve as a safe and effective approach to develop COVID-19 vaccines that provide long-term protection.The roles of fear of COVID-19 and religious coping were examined in relation to depression, anxiety, and stress. Participants (N = 259; 88% female; Mage = 32.96, SD = 8.88) completed measures of fear of COVID-19, religious coping, depression, anxiety, and stress. Results showed that fear of COVID-19 was associated with depression, anxiety, and stress. Negative religious coping was associated with depression, anxiety, and stress while positive religious coping was only associated with depression and stress. Fear of COVID-19 and negative religious coping may be detrimental to mental health while positive religious coping may reduce depression and stress.In this study, Li+ biosorption profiles of Micrococcus luteus and Bacillus pumilus bacterial strains were investigated. Comparative surface characterization of the biomasses revealed that B. pumilus had a significantly greater surface negativity than the other, which had a direct positive effect on the ability to attract the Li+ ions. Biosorption experiments showed that B. pumilus cell had more efficient performance at all pH and initial Li+ concentration values in the ranges of 3.0-10.0 and 2.5-20.0 mg/L, respectively. The maximum adsorption capacities obtained at initial Li+ concentration of 20.0 mg/L and pH 9.0 were 1.160 mg Li+/g (167.1 µmol/g) and 2.280 mg Li+/g (328.5 µmol/g) for M. luteus and B. pumilus, respectively. For all the cases studied, the biosorption equilibrium was reached very quickly, suggesting that physical interaction dominated this process. Experimental data were found to be compatible with both Langmuir and Freundlich models under the studied experimental conditions. This study highlights the idea that B. pumilus bacterial strain will be a new and preferred biosorbent for Li+ ions by providing a low cost, rapid and quite efficient process. This study evaluated the efficacy of using a hybrid closed loop (HCL) system in restoring hypoglycemia awareness in individuals with impaired awareness of hypoglycemia (IAH). Participants with IAH (Gold Score ≥4) were recruited into a randomized crossover pilot study. They participated in two 8-week periods using a HCL system (Medtronic 670GTM) (intervention) and standard insulin pump therapy (control). Hyperinsulinemic hypoglycemic clamp studies were undertaken at baseline and at the end of each study period for the evaluation of the counter-regulatory hormonal and symptomatic responses to hypoglycemia. Seventeen participants (mean age (SD) 35.8y (11.2y)) were included in the study. Peak epinephrine levels (median, IQR) in response to hypoglycemia were similar post intervention and control periods; 234.7 pmol/L (109.2; 938.9) vs 188.3 pmol/L (133.7; 402.9), p=0.233. However, both peak adrenergic and neuroglycopenic symptom scores were higher after intervention; 5.0 (4.5; 9.0) vs 4.0 (4.0; 5.5), p=0.009uggest the potential benefits of a HCL system in people with IAH.Background Patients with breast cancer-related lymphedema (BCRL) have lower quality of life (QOL). However, some important predictors, such as the effect of age, lymphedema severity, depression, and anxiety, have not yet been discovered. The overall objective of this study is to explore the QOL predictors associated with BCRL in China. Methods and Results A cross-sectional design was conducted. Data were collected before treatment, including sociodemographic characteristics (height, heaviness, age, education level, work status, marital status, and economic status), clinical characteristics (surgical method, clinical cancer stage, lymphedema severity, and lymphedema duration), the hospital anxiety (HA) and depression scale, and the functional assessment of cancer therapy-breast quality of life instrument. Univariate analysis or bivariate correlation was first made to explore the correlation of QOL with sociodemographic/clinical characteristics, anxiety, and depression. The multiple linear regression model was used to identify the independent QOL predictors. Seventy-one patients with BCRL were recruited. Age, education level, work status, family income, lymphedema duration, lymphedema severity, and HA and hospital depression scale scores are significantly correlated with QOL (p  less then  0.05). https://www.selleckchem.com/products/ds-6051b.html Age, lymphedema severity, and HA accounted for 85.9% in QOL (F = 62.76, p  less then  0.001). Conclusions Age, lymphedema, and anxiety are the most important QOL predictors. Therefore, it is very important to establish a BCRL prevention system and pay attention to psychological distress in the patients with BCRL.Aim To assess the agreement between indirect and optoelectronic volumetries to diagnose lymphedema based on arm volume difference in patients with axillary lymph node dissection (ALND) for cutaneous melanoma. Methods and Results Patients were assessed by circumferential girth measurements (truncated cone formula) to determine the upper limb volumes (indirect volumetry) and by optoelectronic volumetry (Perometer®) of affected and control limbs. A diagnosis of lymphedema on each measuring method was defined as an absolute volume difference >200 mL or a relative volume >10%. Forty-six patients with ALND were included. There were no significant differences between the volume means or the mean absolute or relative differences measured by each method. Good correlation was observed between the volume of upper limbs for both the left (r = 0.998) and right (r = 0.985) arms. As for the diagnosis of lymphedema, an absolute volume difference >200 mL determined a prevalence of 28% (13/46) of lymphedema by indirect volumetry and 35% (16/46) by optoelectronics volumetry.