002) with music, as well as a decrease in the amount of pinprick pain (P  less then  0.001) and TSP (P = 0.01) with music. Interestingly, CPM was also significantly diminished (P  less then  0.001) in the music condition. No significant difference in cold pain, anxiety, or situational catastrophizing was observed with music. Higher baseline pain catastrophizing scores were associated with less music-induced pressure pain reduction. CONCLUSIONS Several measures of mechanical pain sensitivity were reduced with music. TSP, a measure of central sensitization, also decreased with music, but CPM, a measure of descending modulation of pain, was not further augmented by music. © The Author(s) 2020. Published by Oxford University Press on behalf of the American Academy of Pain Medicine. All rights reserved. For permissions, please e-mail journals.permissions@oup.com.(word count = 150). Cooperation is necessary for solving numerous social issues, including climate change, effective governance, and economic stability. Value-based decision models contend that prosocial tendencies and social context shape people's preferences for cooperative or selfish behavior. Using functional neuroimaging and computational modeling, we tested these predictions by comparing activity in brain regions previously linked to valuation and executive function during decision-making-the ventromedial prefrontal cortex (vmPFC) and dorsolateral prefrontal cortex (dlPFC), respectively. Participants played Public Goods Games with students from fictitious universities, where social norms were selfish or cooperative. Prosocial participants showed greater vmPFC activity when cooperating and dlPFC-vmPFC connectivity when acting selfishly, whereas selfish participants displayed the opposite pattern. Norm-sensitive participants showed greater dlPFC-vmPFC connectivity when defying group norms. Modeling expectations of cooperation was associated with activity near the right temporoparietal junction. Consistent with value-based models, this suggests prosocial tendencies and contextual norms flexibly determine whether people prefer cooperation or defection. © The Author(s) 2020. Published by Oxford University Press.Endo-β-N-acetylglucosaminidases are enzymes that hydrolyze the N,N'-diacetylchitobiose unit of N-glycans. Many endo-β-N-acetylglucosaminidases also exhibit transglycosylation activity, which corresponds to the reverse of the hydrolysis reaction. https://www.selleckchem.com/products/sb297006.html Because of these activities, some of these enzymes have recently been used as powerful tools for glycan remodeling of glycoproteins. Although many endo-β-N-acetylglucosaminidases have been identified and characterized to date, there are few enzymes that exhibit hydrolysis activity toward multi-branched (tetra-antennary or more) complex-type N-glycans on glycoproteins. Therefore, we searched for novel endo-β-N-acetylglucosaminidases that exhibit hydrolysis activity toward multi-branched complex-type N-glycans in this study. From database searches, we selected three candidate enzymes from Tannerella species-Endo-Tsp1006, Endo-Tsp1263, and Endo-Tsp1457-and prepared them as recombinant proteins. We analyzed the hydrolysis activity of these enzymes toward N-glycans on glycoproteins and found that Endo-Tsp1006 and Endo-Tsp1263 exhibited hydrolysis activity toward complex-type N-glycans, including multi-branched N-glycans, preferentially, whereas Endo-Tsp1457 exhibited hydrolysis activity toward high-mannose-type N-glycans exclusively. We further analyzed substrate specificities of Endo-Tsp1006 and Endo-Tsp1263 using 18 defined glycopeptides as substrates, each having a different N-glycan structure. We found that Endo-Tsp1006 preferred N-glycans with galactose or α2,6-linked sialic acid residues in their non-reducing ends as substrates, whereas Endo-Tsp1263 preferred N-glycans with N-acetylglucosamine residues in their non-reducing ends as substrates. © The Author(s) 2020. Published by Oxford University Press. All rights reserved. For permissions, please e-mail journals.permissions@oup.com.Efforts aimed at increasing the pace of evidence synthesis have been primarily focused on the use of published articles, but these are a relatively delayed, incomplete, and at times biased source of study results data. Compared to those in bibliographic databases, structured results data available in trial registries may be more timely, complete, and accessible, but these data remain underutilized. Key advantages of using structured results data include the potential to automatically monitor the accumulation of relevant evidence and use it to signal when a systematic review requires updating, as well as to prospectively assign trials to already published reviews. Shifting focus to emerging sources of structured trial data may provide the impetus to build a more proactive and efficient system of continuous evidence surveillance. © The Author(s) 2020. Published by Oxford University Press on behalf of the American Medical Informatics Association. All rights reserved. For permissions, please email journals.permissions@oup.com.OBJECTIVES To determine antibiotic susceptibility of Streptococcus pneumoniae and Haemophilus influenzae isolates from community-acquired respiratory tract infections (CA-RTIs) collected in 2015-17 from Turkey. METHODS MICs were determined by CLSI broth microdilution and susceptibility was assessed using CLSI, EUCAST (dose-specific) and pharmacokinetic/pharmacodynamic (PK/PD) breakpoints. RESULTS A total of 179 S. pneumoniae and 239 H. influenzae isolates were collected. Few (27.9%) pneumococci were penicillin susceptible by CLSI oral or EUCAST low-dose breakpoints, but by EUCAST high-dose or CLSI IV breakpoints 84.4% were susceptible. The most active antibiotics (excluding penicillin IV) by CLSI breakpoints were fluoroquinolones (98.9% of isolates susceptible), ceftriaxone (83.2%), amoxicillin (78.8%) and amoxicillin/clavulanic acid (78.8%). Pneumococcal susceptibility to amoxicillin and amoxicillin/clavulanic acid was lower using EUCAST low-dose breakpoints (49.7%), although susceptibility increased when using EUCAST high-dose (57.